Identification and Quantitation of Zolpidem in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS)

Abstract

Zolpidem is a short-acting nonbenzodiazepine hypnotic used for the treatment of insomnia. It works quickly and has a short half-life of 2-3 h. Significant side effects are reported including dizziness, amnesia, and even hallucinations. In this method, zolpidem is extracted from blood or other biological fluids/tissues using solid phase extraction technology for analysis. The extract is then assayed using selected ion monitoring gas chromatography/mass spectrometry for absolute confirmation of the compound. The quantitation of this drug is made from standard curves constructed using selected ion monitoring and peak-area ratios of analyte vs. internal standard of quantifying ions.

Валидация Методики Количественного Определения Золпидема В Цельной Крови Методом Газовой Хроматомасс-Спектрометрии

“…Ïîýòîìó èäåíòèôèêàöèÿ äàííîãî ïðåïàðàòà â áèî-ëîãè÷åñêîì ìàòåðèàëå ñ êîëè÷åñòâåííîé îöåíêîé ñîäåðaeàíèÿ åãî â êðîâè ÷ðåçâû÷àéíî âàaeíà äëÿ õèìèêî-òîêñèêîëîãè÷åñêîãî è ñóäåáíî-õèìè÷åñêîãî àíàëèçà. Äëÿ ýòîé öåëè ñóùåñòâóþò ìåòîäèêè ñ èñïîëüçîâàíèåì ãàçîâîé [10,11] è aeèäêîñòíîé [12,13] õðîìàòîãðàôèè ñ ïðèìåíåíèåì ðàçëè÷íûõ ìåòîäîâ äåòåêòèðîâàíèÿ, èììóíîàíàëèç [14]. Ïðèìåíåíèå òîé èëè èíîé ìåòîäèêè çàâèñèò îò òåõíè÷åñêîãî è ìàòåðèàëüíîãî îñíàùåíèÿ ëàáîðàòîðèè, ñëîaeèâøèõñÿ òðàäèöèîííûõ ïðåäïî÷òåíèé.…”