Identification Keratin 1 as a cDDP-resistant protein in nasopharyngeal carcinoma cell lines

Tang, Sanyuan; Huang, Weiguo; Zhong, Meizuo; Yin, Leilan; Jiang, Hao; Suping, Hou; Gan, Pingping; Yuan, Yujuan

doi:10.1016/j.jprot.2012.02.003

Cited by 27 publications

(23 citation statements)

References 28 publications

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“…understanding the molecular basis for development of multi-drug chemoresistance in DLBCL may serve as a basis for identification of novel therapeutic targets and biomarkers involved in DLBCL resistance to CHOP. Previous studies have suggested that Annexin A5, a calcium-dependent phospholipid-binding protein, while promoting the malignancy and chemoresistance in certain types of cancer, is negatively correlated with those in other cancers, including DLBCL (9)(10)(11)(12). In the present study, we demonstrated an important inhibitory role for Annexin A5 in DLBCL cell invasion and chemoresistance to CHOP.…”

Section: Discussionsupporting

confidence: 67%

“…Annexin A5 is negatively correlated with the malignancy and chemoresistance in some cancers, while it enhances those in other cancers (9)(10)(11)(12). Our study demonstrated an inhibitory effect of Annexin A5 on DLBCL chemoresistance, while previous studies have shown that Annexin A5 enhances chemoresistance in gastric cancer and nasopharyngeal carcinoma (10,11).…”

Section: Discussionsupporting

confidence: 44%

“…Nevertheless, upregulation of Annexin A5 has also been found to be negatively correlated with the malignancy of thyroid cancer (9). Previous studies suggested that Annexin A5 enhances chemoresistance in gastric cancer and nasopharyngeal carcinoma (10,11). However, a recent study showed that Annexin A5 was upregulated in CHOP-sensitive DLBCL tissues, suggesting that Annexin A5 may inhibit chemoresistance in DLBCL (12).…”

Section: Introductionmentioning

confidence: 99%

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Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling

Wang¹,

Zhang²,

Liu³

et al. 2014

Oncology Reports

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Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin's lymphoma worldwide. Although patient outcomes have significantly improved to a greater than 40% cure rate by the combinatorial cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) chemotherapy, which is widely used, resistance to the CHOP regimen continues to pose a problem in managing or curing DLBCL. While it promotes the malignancy and chemo-resistance in certain types of cancer, Annexin A5 is negatively correlated with those in other cancers, including DLBCL. In the present study, we explored the effects of Annexin A5 on DLBCL cell invasion and chemoresistance to CHOP. Stable overexpression and knockdown of Annexin A5 were performed in Toledo and Pfeiffer human DLBCL cell lines. Overexpression of Annexin A5 in both cell lines significantly decreased cell invasion, matrix metalloproteinase-9 (MMP-9) expression/activity, phosphatidylinositol 3-kinase (PI3K) activity/Akt phosphorylation, and cell survival against CHOP-induced apoptosis. On the other hand, knockdown of Annexin A5 markedly increased cell invasion, MMP-9 expression/activity, PI3K activity/Akt phosphorylation, and CHOP-induced apoptosis in the DLBCL cell lines, which was abolished by selective PI3K inhibitor BKM120. In conclusion, our study provides the first in vitro evidence that Annexin A5 inhibits DLBCL cell invasion, MMP-9 expression/activity, and chemoresistance to CHOP through a PI3K-dependent mechanism; it provides new insight not only into the biological function of Annexin A5, but also into the molecular mechanisms underlying DLBCL progression and chemoresistance.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionsupporting

confidence: 44%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling

Wang¹,

Zhang²,

Liu³

et al. 2014

Oncology Reports

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“…In addition, proteomic studies allowed the identification of several cytoskeletal proteins modulated by acute cisplatin treatment or differentially expressed in cells sensitive or resistant to Pt compounds (Castagna et al, 2004;Vasko et al, 2011). For instance, up-regulation of Keratin1 (KRT1), a protein known to induce cytoskeletal stabilization, has been described to be functionally connected with cisplatin resistance in human nasopharyngeal and ovarian carcinoma cell lines (Tang et al, 2012;Zhou et al, 2012).…”

Section: Interactions Of Platinum Compounds With the Cytoskeletonmentioning

confidence: 99%

New mechanisms for old drugs: Insights into DNA-unrelated effects of platinum compounds and drug resistance determinants

Gatti

Cassinelli

Zaffaroni

et al. 2015

Drug Resistance Updates

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“…Use of the mouse xenotransplantation model was aborted due to substantial mouse protein content, and human body fluid was limited due to its formidable heterogenicity. Thus, cell lines were considered to be more valuable in proteomic studies due to their low complexity and variability of proteins with which to explore NPC-related proteins (15). CNE-2 is a low-differentiated NPC epithelioid cell line derived from a primary tumor biopsy in China in 1980 (16).…”

Section: Analysis Of the Differential Secretome Of Nasopharyngeal Carmentioning

confidence: 99%

Analysis of the differential secretome of nasopharyngeal carcinoma cell lines CNE-2R and CNE-2

Chen

Guo

et al. 2015

Oncology Reports

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Radioresistance is the major cause of poor prognosis in nasopharyngeal carcinoma (NPC). To identify and characterize the secretome associated with NPC radioresistance, we compared the conditioned serum-free medium of radioresistant CNE-2R cells with that of the parental radiosensitive CNE-2 cells using isobaric tags for relative and absolute quantitation (iTRAQ) with liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) quantitative proteomics. Before proceeding to quantitative proteomics, we investigated the survival curves of CNE-2R and CNE-2 cells by colony formation assay, and the CNE-2R survival curves were significantly higher than those for CNE-2. In total, 3,581 proteins were identified in the quantitative proteomics experiments, and 40 proteins exhibited significant differences between the CNE-2R and CNE-2 cells. Twenty-six of the 40 proteins were secreted by classical, non-classical, or exosomal secretion pathways. To verify the reliability of iTRAQ quantitative proteomics, we applied western blotting (WB) to study the secretory protein expression of fibrillin-2, CD166, sulfhydryl oxidase 1 and cofilin-2, which are involved in cell adhesion, migration and invasion. The WB results showed that fibrillin-2 (p=0.017) and sulfhydryl oxidase 1 (p=0.000) were highly expressed in the CNE-2 cells, while CD166 (p=0.012) and cofilin-2 (p=0.003) were highly expressed in the CNE-2R cells, which was in accordance with iTRAQ quantitative proteomics. Finally, a phenotypic subset of CD166-positive NPC cells was verified by immunocytochemistry. In summary, we defined a collection of secretory proteins that may be relevant to the radioresistance in NPC cells, and we determined that CD166, which is widely used as a positive marker of cancer stem cells, is expressed in NPC cells.

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Identification Keratin 1 as a cDDP-resistant protein in nasopharyngeal carcinoma cell lines

Cited by 27 publications

References 28 publications

Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling

Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling

New mechanisms for old drugs: Insights into DNA-unrelated effects of platinum compounds and drug resistance determinants

Analysis of the differential secretome of nasopharyngeal carcinoma cell lines CNE-2R and CNE-2

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