1993
DOI: 10.1111/j.1432-1033.1993.tb17620.x
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Identification of a distinct human high‐density lipoprotein subspecies defined by a lipoprotein‐associated protein, K‐45

Abstract: In an attempt to provide immunological tools for subfractionation of high-density lipoproteins (HDL), monoclonal antibodies were raised against HDL complexes. Two clones identified a peptide, provisionally named K-45 (PI 4.5-4.9; molecular mass 45 kDa, range 42-48 kDa), whose plasma distribution and lipoprotein association were fully characterised. Gel filtration localised the peptide to the HDL region of human plasma where it co-eluted with apolipoprotein (apo) A-I, the structural protein of HDL. Complementar… Show more

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Cited by 322 publications
(215 citation statements)
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“…The observation that PON specific activity was dependent on the extent of apoA-I lipidation suggests that the extracellular assembly of nascent HDL may represent an important first step in the discrimination that PON exhibits for specific lipoprotein subclasses. This discrimination is exemplified by the in vivo observations that PON can be found associated with subclasses of HDL composed of apoA-I without apoA-II (15).…”
Section: Discussionmentioning
confidence: 94%
“…The observation that PON specific activity was dependent on the extent of apoA-I lipidation suggests that the extracellular assembly of nascent HDL may represent an important first step in the discrimination that PON exhibits for specific lipoprotein subclasses. This discrimination is exemplified by the in vivo observations that PON can be found associated with subclasses of HDL composed of apoA-I without apoA-II (15).…”
Section: Discussionmentioning
confidence: 94%
“…Human HDL (δ 1.063-1.21 g/ml) was isolated from serum samples by density gradient ultracentrifugation [11], dialyzed against Tris buffer, and sterilely filtered for storage at 4°C. Enzyme activity assays PON1 enzyme activity was analyzed as phenylacetate hydrolysis (arylesterase activity [12]). Cell-associated activity was measured as described previously [10].…”
Section: Preparation Of Recombinant Pon1 and Hdlmentioning
confidence: 99%
“…Three maps were generated: (i) a less crowded one with 639 SCs corresponding to the identified spots in human plasma retrieved from SWISS-2-D-PAGE Map Selection [35][36][37][38][39][40] (Fig. 1); ii) a crowded one generated with 1500 identified spots collected from different reference maps reported in the SWISS- …”
Section: Computer-simulated Mapsmentioning
confidence: 99%