Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene ofSinorhizobium frediiUSDA191 Required formyo-Inositol Utilization

Yoshida, Ken-ichi; Kim, Won‐Seok; Kinehara, Masaki; Mukai, Rie; Ashida, Hitoshi; Ikeda, Hideki; Fujita, Yuki; Krishnan, Hari B.

doi:10.1271/bbb.60362

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…The metabolism of MI in Sinorhizobium fredii and Rhizobium leguminosarum is required for efficient nitrogen fixation and nodulation of soybeans (9,15). MI dehydrogenase genes (e.g., iolG) and inosose dehydratase genes (iolE) have been characterized in S. fredii and Sinorhizobium meliloti (10,15,36), and iolA and iolDEB genes have been studied in R. leguminosarum (9). An MI utilization gene cluster has also been described for Clostridium perfringens, which was induced by MI via the IolR regulator (16), and recently, transcriptome analysis permitted the identification of an iol cluster which allowed the rapid growth of Corynebacterium glutamicum on MI (19).…”

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confidence: 99%

Identification of a Gene Cluster EnablingLactobacillus caseiBL23 To Utilizemyo-Inositol

Yebra¹,

Zúñiga²,

Beaufils

et al. 2007

Appl Environ Microbiol

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Genome analysis of Lactobacillus casei BL23 revealed that, compared to L. casei ATCC 334, it carries a 12.8-kb DNA insertion containing genes involved in the catabolism of the cyclic polyol myo-inositol (MI). Indeed, L. casei ATCC 334 does not ferment MI, whereas strain BL23 is able to utilize this carbon source. The inserted DNA consists of an iolR gene encoding a DeoR family transcriptional repressor and a divergently transcribed iolTABCDG1G2EJK operon, encoding a complete MI catabolic pathway, in which the iolK gene probably codes for a malonate semialdehyde decarboxylase. The presence of iolK suggests that L. casei has two alternative pathways for the metabolism of malonic semialdehyde: (i) the classical MI catabolic pathway in which IolA (malonate semialdehyde dehydrogenase) catalyzes the formation of acetyl-coenzyme A from malonic semialdehyde and (ii) the conversion of malonic semialdehyde to acetaldehyde catalyzed by the product of iolK. The function of the iol genes was verified by the disruption of iolA, iolT, and iolD, which provided MI-negative strains. By contrast, the disruption of iolK resulted in a strain with no obvious defect in MI utilization. Transcriptional analyses conducted with different mutant strains showed that the iolTABCDG1G2EJK cluster is regulated by substrate-specific induction mediated by the inactivation of the transcriptional repressor IolR and by carbon catabolite repression mediated by the catabolite control protein A (CcpA). This is the first example of an operon for MI utilization in lactic acid bacteria and illustrates the versatility of carbohydrate utilization in L. casei BL23.myo-Inositol (MI) is the most abundant stereoisomer of inositol (1,2,3,4,5,6-cyclohexanehexol). It is common in soils, and it is a constituent of phytic acid (inositol hexaphosphate), which in the form of various salts (phytates) provides a major phosphate storage molecule in plant seeds. Several microorganisms, mostly inhabitants of soil, can utilize MI as a carbon source. For bacteria, the MI catabolic pathway has been elucidated for Enterobacter aerogenes (formerly Aerobacter aerogenes) (3). MI is important for the establishment of symbiosis in legume nodules. The metabolism of MI in Sinorhizobium fredii and Rhizobium leguminosarum is required for efficient nitrogen fixation and nodulation of soybeans (9, 15). MI dehydrogenase genes (e.g., iolG) and inosose dehydratase genes (iolE) have been characterized in S. fredii and Sinorhizobium meliloti (10, 15, 36), and iolA and iolDEB genes have been studied in R. leguminosarum (9). An MI utilization gene cluster has also been described for Clostridium perfringens, which was induced by MI via the IolR regulator (16), and recently, transcriptome analysis permitted the identification of an iol cluster which allowed the rapid growth of Corynebacterium glutamicum on MI (19). However, the first MI catabolic gene cluster was characterized in Bacillus subtilis, and detailed molecular data are available only for this bacterium. The B. subtilis iolRS and iolABC...

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confidence: 99%

Identification of a Gene Cluster EnablingLactobacillus caseiBL23 To Utilizemyo-Inositol

Yebra¹,

Zúñiga²,

Beaufils

et al. 2007

Appl Environ Microbiol

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“…2007; Abeysinghe 2009). As growth‐promoting endophytic bacteria, some strains of B. subtilis have a synergistic effect on Rhizobium ‐mediated nodulation and nitrogen fixation (Yoshida et al. 2006; Rinu & Pandey 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have shown that B. subtilis has a close relationship with plants, with the ability to promote plant growth Manjula & Podile 2005;Singh et al 2008;Swain & Ray 2009) and protect plants from pests (Nagó rska et al 2007;Abeysinghe 2009). As growth-promoting endophytic bacteria, some strains of B. subtilis have a synergistic effect on Rhizobiummediated nodulation and nitrogen fixation (Yoshida et al 2006;Rinu & Pandey 2009). Some are capable of fixing nitrogen by themselves (Enkh-Amgalan et al 2006;Jeong et al 2006), and some can promote plant growth or resistance to harsh conditions by secreting indole acetic acid, cytokinins or other phytohormones Estevez et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Endophytic colonisation of Bacillus subtilis in the roots of Robinia pseudoacacia L.

Huang

Zhuang

et al. 2011

Plant Biology

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The endophytic colonisation of Bacillus subtilis strain GXJM08, isolated from roots of Podocarpus imbricatus B1. Enum. P1. Jav., in roots of the leguminous plant Robinia pseudoacacia L. was investigated. Ultrastructure observations showed that B. subtilis caused morphological changes in the root hair and colonised the plant through infected root hairs. The structure of the infection thread was similar to that of rhizobia, but the structure of infected cells was different. B. subtilis is also different from rhizobia and plant pathogens in terms of the formation of a peribacteroid membrane and the mode of penetration through the host cell wall. Our results provide a basis for studying development of the mutualistic symbiotic relationship between B. subtilis and plants, and a basis for studying the mechanism of the B. subtilis-plant interaction.

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“…In other bacteria, function of particular genes such as MI dehydrogenase (iolG) genes and inosose dehydratase (iolE) genes has also been studied in detail (Fujita et al 1991;Makarova et al 2006;Yoshida et al 2004Yoshida et al , 2006a. Genes involved in MI utilization were most often found as a cluster localized in a contiguous genomic segment (Kawsar et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of iol clusters in Lactobacillus casei strains

Zhang

Sun

Yü

et al. 2010

World J Microbiol Biotechnol

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The present study was designed to expand genetic knowledge of myo-inositol (MI) metabolism in Lactobacillus casei. Twenty-four L. casei isolates of dairy origin were tested for the presence of iol cluster. PCR screening revealed eight strains encoded functions involved in MI utilization, of which one strain was able to use MI as carbon source. To gain a deeper understanding of the function of iol genes, four of the eight observed iol clusters were subjected to the full sequencing procedure. The results showed that the iol cluster was not a common feature among dairy L. casei strains. In addition, the four iol clusters were highly similar to one another in terms of sequence similarity and operon architecture. However, abundant polymorphisms that comprised a majority of synonymous mutations were detected throughout the full sequences. Three of them distributed among iolB, iolC, and iolT genes were found in linkage to MI-negative phenotype. Compared with other bacterial iol clusters, the iol cluster of L. casei showed a high similarity with that of Bacillus subtilis.

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Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene ofSinorhizobium frediiUSDA191 Required formyo-Inositol Utilization

Cited by 5 publications

References 32 publications

Identification of a Gene Cluster EnablingLactobacillus caseiBL23 To Utilizemyo-Inositol

Identification of a Gene Cluster EnablingLactobacillus caseiBL23 To Utilizemyo-Inositol

Endophytic colonisation of Bacillus subtilis in the roots of Robinia pseudoacacia L.

Comparative analysis of iol clusters in Lactobacillus casei strains

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