Identification of a membrane glycoprotein associated with haemopoietic progenitor cells

Katz, F; Tindle, Robert W.; Sutherland, D. Robert; Greaves, Melvyn F.

doi:10.1016/0145-2126(85)90082-7

Cited by 169 publications

(72 citation statements)

References 14 publications

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“…In order to examine the expression of human CD34 in committed and mature cells we performed 2-color staining for surface markers defining mature hematopoietic cells. We found that expression of the exogenous human CD34 gene decreased with cell maturation in concordance with published studies of the expression of the endogenous murine CD34 gene during ongoing cell maturation, 1,2,8,[45][46][47] whereas double-positive populations were found when staining for Sca-1 and c-kit, markers found on cells corresponding to a more immature phenotype. 48,49 A notable exception was the coexpression with the B-cell-specific marker B220 on a subset of cells.…”

Section: Expression Of the Transgene In The Bone Marrow Decreases Witsupporting

confidence: 80%

Transgenic targeting with regulatory elements of the humanCD34 gene

Radomska

González

Okuno

et al. 2002

Blood

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Section: Expression Of the Transgene In The Bone Marrow Decreases Witsupporting

confidence: 80%

Transgenic targeting with regulatory elements of the humanCD34 gene

Radomska

González

Okuno

et al. 2002

Blood

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“…Similarly, HSCs express CD34, KDR, and Tie-2/Tek on their surface (51)(52)(53). CD34, however, is lost by HSCs as they differentiate into mature blood cells (54)(55)(56). In an early study, Rafii et al demonstrated colonization of the flow surface of the titanium housing of left ventricular assist devices with CD34 + EC-like cells 6 months after the devices were removed (57).…”

Section: Discussionmentioning

confidence: 99%

Transplanted cord blood–derived endothelial precursor cells augment postnatal neovascularization

Murohara

Ikeda²,

Duan³

et al. 2000

J. Clin. Invest.

849

654

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MethodsHuman umbilical cord blood. Human umbilical cord blood samples (50-140 mL each; n = 102) were collected in sterile blood packs (SC-200; Terumo Corp., Tokyo, Japan) containing citrate-dextrose solution as the anticoagulant. Written informed consent was obtained from all mothers before labor and delivery. Protocols for sampling human umbilical cord blood were approved by the Institutional Review Board. Endothelial precursor cells (EPCs) have been identified in adult peripheral blood. We examined whether EPCs could be isolated from umbilical cord blood, a rich source for hematopoietic progenitors, and whether in vivo transplantation of EPCs could modulate postnatal neovascularization. Numerous cell clusters, spindle-shaped and attaching (AT) cells, and cord-like structures developed from culture of cord blood mononuclear cells (MNCs). Fluorescence-trace experiments revealed that cell clusters, AT cells, and cord-like structures predominantly were derived from CD34-positive MNCs (MNC CD34+ ). AT cells and cell clusters could be generated more efficiently from cord blood MNCs than from adult peripheral blood MNCs. AT cells incorporated acetylated-LDL, released nitric oxide, and expressed KDR, VE-cadherin, CD31, and von Willebrand factor but not CD45. Locally transplanted AT cells survived and participated in capillary networks in the ischemic tissues of immunodeficient nude rats in vivo. AT cells thus had multiple endothelial phenotypes and were defined as a major population of EPCs. Furthermore, laser Doppler and immunohistochemical analyses revealed that EPC transplantation quantitatively augmented neovascularization and blood flow in the ischemic hindlimb. In conclusion, umbilical cord blood is a valuable source of EPCs, and transplantation of cord blood-derived EPCs represents a promising strategy for modulating postnatal neovascularization.

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“…Since the frequency ofvarious hematopoietic progenitor cells appears to be directly related to the degree oftheir cellular differentiation, one would hypothesize that the frequency in normal bone marrow of CFU-B1 is extremely low. To facilitate the enrichment of CFU-B 1, a number of physical and immunological characteristics ofthese cells could be utilized (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). Another means of obtaining enriched populations of CFU-BI might be to pharmacologically purge the marrow of more differentiated elements by in vitro exposure to chemotherapeutic agents (29,30).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a human hematopoietic progenitor cell capable of forming blast cell containing colonies in vitro.

et al. 1988

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A hematopoietic cell (CFU-B1) capable of producing blast cell containing colonies in vitro was detected using a semisolid culture system. The CFU-B1 has the capacity for self-renewal and commitment to a number of hematopoietic lineages. Monoclonal antibody to the human progenitor cell antigen-i (HPCA-1) and a monoclonal antibody against the major histocompatibility class II antigen (HLA-DR) were used with fluorescence activated cell sorting to phenotype the CFU-B1. The CFU-B1 was found to express MylO but not HLA-DR antigen; experiments using complement-dependent cytotoxicity to eliminate DR positive cells confirmed this finding. Pretreatment of marrow cells with two chemotherapeutic agents, 5-fluorouracil and 4-hydroperoxycyclophosphamide facilitated detection of CFU-B1 derived colonies, while diminishing or totally inhibiting colony formation by other hematopoietic progenitor cells. CFU-Bl-derived colony formation was dependent upon the addition of exogenous hematopoietic growth factors. Media conditioned either by the human bladder carcinoma cell line 5637 or lectin stimulated leukocytes, as well as recombinant granulocyte-macrophage colony stimulating factor, interleukin 3 or interleukin la promoted blast cell colony formation. By contrast, neither recombinant erythropoietin, recombinant interleukin 4, purified macrophage colony stimulating factor or recombinant granulocyte colony-stimulating factor alone promoted blast cell colony formation.

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Identification of a membrane glycoprotein associated with haemopoietic progenitor cells

Cited by 169 publications

References 14 publications

Transgenic targeting with regulatory elements of the humanCD34 gene

Transgenic targeting with regulatory elements of the humanCD34 gene

Transplanted cord blood–derived endothelial precursor cells augment postnatal neovascularization

Characterization of a human hematopoietic progenitor cell capable of forming blast cell containing colonies in vitro.

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