Identification of a novel bovine copiparvovirus in pooled fetal bovine serum

Baylis, Sally A.; Miskey, Csaba; Blümel, Johannes; Kaiser, Marco; Kapusinszky, Beatrix; Delwart, Eric

doi:10.1007/s11262-020-01757-1

Cited by 5 publications

(7 citation statements)

References 22 publications

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“…FBS is subjected to important lot-to-lot variability depending on diet, geographical location, season and fetal calves gestational age [31,32], leading to major challenges in data reproducibility and increased costs in order to test each new serum lot. FBS use raises safety concerns for patients in terms of the potential presence of infectious contaminants [33,34]. FBS has also been associated with clinical immunogenic implications related to the xenogeneic bovine proteins [35].…”

Section: Introductionmentioning

confidence: 99%

Engineering naturally-derived human connective tissues for clinical applications using a serum-free production system

et al. 2022

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The increasing need for tissue substitutes in reconstructive surgery spurs the development of engineering methods suited for clinical applications. Cell culture and tissue production traditionally require the use of fetal bovine serum (FBS) which is associated with various complications especially from a translational perspective. Using the self-assembly approach of tissue engineering, we hypothesized that all important parameters of tissue reconstruction can be maintained in a production system devoid of FBS from cell extraction to tissue reconstruction. We studied two commercially available serum-free medium (SFM) and xenogen-free serum-free medium (XSFM) for their impact on tissue reconstruction using human adipose-derived stem/stromal cells (ASCs) in comparison to serum-containing medium. Both media allowed higher ASC proliferation rates in primary cultures over five passages compared with 10% FBS supplemented medium while maintaining high expression of mesenchymal cell markers. For both media, we evaluated extracellular matrix production and deposition necessary to engineer manipulatable tissues using the self-assembly approach. Tissues produced in SFM exhibited a significantly increased thickness (up to 6.8-fold) compared with XSFM and FBS-containing medium. A detailed characterization of tissues produced under SFM conditions showed a substantial 50% reduction of production time without compromising key tissue features such as thickness, mechanical resistance and pro-angiogenic secretory capacities (plasminogen activator inhibitor 1, hepatocyte growth factor, vascular endothelial growth factor, angiopoietin-1) when compared to tissues produced in the control FBS-containing medium. Furthermore, we compared ASCs to the frequently used human dermal fibroblasts (DFs) in the SFM culture system. ASC-derived tissues displayed a 2.4-fold increased thickness compared to their DFs counterparts. In summary, we developed all-natural human substitutes using a production system compatible with clinical requirements. Under culture conditions devoid of bovine serum, the resulting engineered tissues displayed similar and even superior structural and functional properties over the classic FBS-containing culture conditions with a considerable 50% shortening of production time.

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Section: Introductionmentioning

confidence: 99%

Engineering naturally-derived human connective tissues for clinical applications using a serum-free production system

et al. 2022

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“…Hwang et al (2007) developed an accurate multiplex PCR assay or the rapid screening of the 19 genes that encode staphylococcal enterotoxins (SEs) (sea to see, and seg to sei), SE-like (SEl) toxins (sej to ser, and seu), and toxic shock syndrome toxin-1 (TSST-1) (tst) in pork and chicken meats. Using droplet digital PCR (ddPCR) and DNA sequencing, Baylis et al (2020) detected a previously undiscovered bovine copiparvovirus highly concentrated in pooled samples of FBS. Their analysis of FBS batches revealed a substantial load of approximately 50,000 copies per milliliter of this novel parvoviral DNA.…”

Section: Pcr Methods For Microbial Hazard Detectionmentioning

confidence: 99%

Microbiological and chemical hazards in cultured meat and methods for their detection

Sogore,

Guo,

Sun

et al. 2024

Comp Rev Food Sci Food Safe

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Cultured meat, which involves growing meat in a laboratory rather than breeding animals, offers potential benefits in terms of sustainability, health, and animal welfare compared to conventional meat production. However, the cultured meat production process involves several stages, each with potential hazards requiring careful monitoring and control. Microbial contamination risks exist in the initial cell collection from source animals and the surrounding environment. During cell proliferation, hazards may include chemical residues from media components such as antibiotics and growth factors, as well as microbial issues from improper bioreactor sterilization. In the differentiation stage where cells become muscle tissue, potential hazards include residues from scaffolding materials, microcarriers, and media components. Final maturation and harvesting stages risk environmental contamination from nonsterile conditions, equipment, or worker handling if proper aseptic conditions are not maintained. This review examines the key microbiological and chemical hazards that must be monitored and controlled during the manufacturing process for cultured meats. It describes some conventional and emerging novel techniques that could be applied for the detection of microbial and chemical hazards in cultured meat. The review also outlines the current evolving regulatory landscape around cultured meat and explains how thorough detection and characterization of microbiological and chemical hazards through advanced analytical techniques can provide crucial data to help develop robust, evidence‐based food safety regulations specifically tailored for the cultured meat industry. Implementing new digital food safety methods is recommended for further research on the sensitive and effective detection of microbiological and chemical hazards in cultured meat.

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“…Second-generation sequencing technologies, including sequencing by synthesis and pyrosequencing platforms, provide the ability to detect known and unknown viruses in pooled samples [6][7][8][9]. These technologies associated with the enrichment of viral nucleic acid potentially increase the sequencing sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…These technologies associated with the enrichment of viral nucleic acid potentially increase the sequencing sensitivity. For instance, an evaluation of the virome in FBS samples used the Klenow enzyme for DNA amplification and characterized sequences originating from eukaryotic viruses [6]. On the other hand, multiple displacement amplification (MDA) was used to identify specific viruses and evaluate the test sensitivity in biological samples [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, we characterized the viral population in non-gamma irradiated bovine serum batches from Mexico, the USA, and New Zealand. The serum batches were commercially available in the USA and were subjected to a combination of steps previously described to enrich viral nucleic acid in the samples before sequencing [6,7,[9][10][11][12]. Results demonstrated the presence of sequences related to several eukaryotic viruses from a wide range of animal species and a great diversity of phages, including members of the recently discovered group of cross-assembly phage (CrAssphage).…”

Section: Introductionmentioning

confidence: 99%

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Virome Characterization in Commercial Bovine Serum Batches—A Potentially Needed Testing Strategy for Biological Products

Paim

Maggioli

Falkenberg

et al. 2021

Viruses

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Bovine serum has been widely used as a universal supplement in culture media and other applications, including the manufacture of biological products and the production of synthetic meat. Currently, commercial bovine serum is tested for possible viral contaminants following regional guidelines. Regulatory agencies’ established tests focused on detecting selected animal origin viruses and are based on virus isolation, immunofluorescence, and hemadsorption assays. However, these tests may fail to detect new or emerging viruses in biological products. High-throughput sequencing is a powerful option since no prior knowledge of the viral targets is required. In the present study, we evaluate the virome of seven commercial batches of bovine serum from Mexico (one batch), New Zealand (two batches), and the United States (four batches) using a specific preparation and enrichment method for pooled samples and sequencing using an Illumina platform. A variety of circular replicase-encoding single-stranded (CRESS) DNA families (Genomoviridae, Circoviridae, and Smacoviridae) was identified. Additionally, CrAssphage, a recently discovered group of bacteriophage correlated with fecal contamination, was identified in 85% of the tested batches. Furthermore, sequences representing viral families with single-stranded DNA (Parvoviridae), double-stranded DNA (Polyomaviridae and Adenoviridae), single-stranded RNA (Flaviviridae, Picornaviridae, and Retroviridae), and double-stranded RNA (Reoviridae) were identified. These results support that high-throughput sequencing associated with viral enrichment is a robust tool and should be considered an additional layer of safety when testing pooled biologicals to detect viral contaminants overlooked by the current testing protocols.

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Identification of a novel bovine copiparvovirus in pooled fetal bovine serum

Cited by 5 publications

References 22 publications

Engineering naturally-derived human connective tissues for clinical applications using a serum-free production system

Engineering naturally-derived human connective tissues for clinical applications using a serum-free production system

Microbiological and chemical hazards in cultured meat and methods for their detection

Virome Characterization in Commercial Bovine Serum Batches—A Potentially Needed Testing Strategy for Biological Products

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