Identification of a novel co-transcription of P450/1A1 with telomerase in A549

Shervington, Amal; Mohammed, Kulthum Karama; Patel, Rahima; Lea, Robert

doi:10.1016/j.gene.2006.10.010

Cited by 10 publications

(9 citation statements)

References 46 publications

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“…Previously, we have shown that CYP1A1 and hTERT were transcribed concomitantly in alveolar adenocarcinoma cell line A549 [6]. We confirmed this co-transcription by chemically induced CYP1A1, which resulted in the induction of hTERT.…”

Section: Discussionsupporting

confidence: 80%

“…The cells were transfected with the oligos individually using siPROT Amine for 24 or 48 hours. QRT-PCR results obtained from these experiments showed that siRNAs I and II delivered to the cells using siPORT Amine transfection agent were capable of completely silencing CYP1A1 transcription after 24 hrs, and siRNA III knocked down CYP1A1 transcription by 91% (p<0.001) [6]. While, siRNA I and III achieved a 100% transcriptional knockdown of CYP1A1 after 48 hrs transfection, siRNA II downregulated CYP1A1 by 97% (p<0.001).…”

Section: Resultsmentioning

confidence: 90%

“…The novel constitutive co-transcription of CYP1A1 and hTERT in the alveolar epithelial cancer cell line (A549) was previously reported from our laboratory [6]. In order to assess the role of CYP1A1 in maintaining the upregulation of hTERT, siRNA was used to downregulate CYP1A1 in A549 cells.…”

Section: Resultsmentioning

confidence: 99%

“…A549 cells (3x10 5 ) were subcultured in 4x4 well plates for 24 hrs under ordinary culture conditions before transfection. SiPORT Amine (Ambion, UK) mediated transient co-transfection of CYP1A1 siRNAs was performed in duplicate as previously described [6].…”

Section: Cell Culture and Transfectionmentioning

confidence: 99%

“…A recent publication from our laboratory has reported novel co-transcription activity between two genes namely; CYP1A1 and telomerase (hTERT) in A549 lung cancer cells [6]. CYP1A1 is an extrahepatic enzyme which catalyses the bioactivation of polyaromatic hydrocarbons (PAH) constituents abundant in tobacco smoke into mutagens and carcinogens [7,8].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Can CYP1A1 siRNA be an effective treatment for lung cancer?

Mohammed

Shervington

2008

Cellular and Molecular Biology Letters

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Previously, we identified a novel correlation between the upregulated expression of telomerase (hTERT) and cytochrome P450 1A1 (CYP1A1) in A549 human lung cancer cell line. The expression correlation was confirmed by silencing CYP1A1 expression using siRNA technology and observing a silencing of hTERT transcription. Furthermore, silencing CYP1A1 and subsequently downregulating hTERT resulted in the reduction of cancer cell viability by more than 40%, which appeared as early as 24 hours after the treatment. The concomitant downregulation of CYP1A1 and hTERT resulted in rapid cell death. This finding can be further exploited to develop new molecular targets for the treatment of lung cancer.

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Section: Discussionsupporting

confidence: 80%

Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Cell Culture and Transfectionmentioning

confidence: 99%