Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis

Park, Sun Young; Kim, Sun Gun; Park, Da Jung; Kang, Jum-Soon; Lee, Sang Joon; Yoon, Sik; Kim, Young Hun; Bae, Yoe‐Sik; Choi, Yang-Ho

doi:10.1016/j.bbrc.2009.12.131

Cited by 28 publications

(20 citation statements)

References 23 publications

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“…These include cubenol, elixene, caryophyllene oxide, α-gurjenene and α-farnesene, respectively (Lee et al, 2010;Li et al, 2014;Rajeswari et al, 2011;González et al, 2014;Lavanya et al, 2014;Yang et al, 2014). Epizonarene and santolina epoxide have been reported to exhibit antidiabetic and cardiovascular disorder, respectively (Keskes et al, 2016;Rasheed et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Mystroxylon aethiopicum chloroform root bark extracts phytochemical analysis using gas chromatography mass spectrometry

Kilonzo¹,

Ndakidemi²,

Chacha³

2017

J. Pharmacognosy Phytother.

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Mystroxylon aethiopicum has been used by many ethnic groups in Africa for the management of hemorrhagic diarrhea, stomachache, respiratory tract infections, urinary tract infections coughs, hypertension and gonorrhea. This study was carried out to identify low molecular weight phytochemicals present in the root bark extract of M. aethiopicum with the aid of gas chromatographymass spectrometry (GC-MS) technique. The GC-MS analysis revealed the presence of various low molecular weight phytochemicals which belongs to four groups of secondary metabolites namely sesquiterpenes, dieterpenes, monoterpenes and fatty acids. The presence of these phytochemicals in the plant extract may be positively associated with pharmacological properties of M. aethiopicum and therefore justifying the ethnomedical usage of the plant.

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Section: Discussionmentioning

confidence: 99%

Mystroxylon aethiopicum chloroform root bark extracts phytochemical analysis using gas chromatography mass spectrometry

Kilonzo¹,

Ndakidemi²,

Chacha³

2017

J. Pharmacognosy Phytother.

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“…These features have revealed the potential of α-isocubebene as an effective novel anti-inflammatory agent for the prevention and treatment of vascular diseases. However, the principal biological function of αIC remains unclear, with the exception of the anti-inflammatory effect against bacterial infection or endotoxin recently reported by our group (7). The present study sought to clarify the function of αIC in the context of therapy for hepatocellular carcinoma.…”

Section: Introductionmentioning

confidence: 91%

The α-iso-cubebenol compound isolated from Schisandra chinensis induces p53-independent pathway-mediated apoptosis in hepatocellular carcinoma cells

Kim

Goo

et al. 2012

Oncol Rep

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Abstract. Schisandra chinensis (S. chinensis) plants areextensively used because of their anticancer, anti-inflammatory, antioxidant and antihepatic activities. However, their active compounds remain to be clearly determined. In this study, we investigated the antitumor functions of α-iso-cubebenol (αIC) isolated from S. chinensis using HepG2 hepatocellular carcinoma cells. HepG2 cells were exposed to αIC for 24 h, and apoptosis was assessed using standard viability and cell proliferation assays, flow cytometry and western blotting. HepG2 cell populations treated only with 340 µM of αIC showed markedly increased cell death, but lower concentrations induced minimal alterations of population viability and cell morphology. However, the results of flow cytometry showed that the majority of viable cells were undergoing apoptosis at all tested αIC concentrations. Western blot analysis results revealed a significant and αIC concentration-dependent reduction in the levels of the pro-caspase-3 apoptotic protein and the Bcl-2 anti-apoptotic protein. In particular, the Bax pro-apoptosis protein and p53 (which regulates Bax expression) showed different expression patterns after the application of αIC treatment to HepG2 cells. Bax expression was slightly increased in cells treated with the high concentration of αIC, while p53 expression was markedly reduced in a dose-dependent fashion, similar to that of Bcl-2. The results of this study suggest that αIC is an anticancer drug candidate by virtue of its apoptotic induction abilities in hepatocellular carcinoma cells, which occur via a p53-independent pathway.

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“…Fraction 9 (KH9, 4866 mg) was separated on a silica gel column (100 × 3.0 cm) with a step gradient (1%, 10%, and 15%) of acetone in CHCl 3 to obtain 21 fractions. Next, fraction 2 (KH9IG, 529.9 mg) was separated on a silica gel column (100 × 3.0 cm) with 5% acetone in CHCl 3 to yield α-isocubebenol (162.9 mg) as previously described by [5]. Pure α-isocubebenol was identified by high-performance liquid chromatography (HPLC) on a Luna C18 column (150 × 4.6 mm internal diameter (ID); 5 μm particle size; Phenomenex, LA, CA, USA) with a methanolacetonitrile gradient, at a flow rate of 1.0 mL/min.…”

Section: Extraction Of a New α-Iso-cubebenolmentioning

confidence: 99%

“…Schisandra chinensis and its constituent dibenzo-cycloctadilene lignans have been reported to have anti-oxidative, anti-inflammatory, antitumor, hepatoprotective, and neuroprotective effects [1][2][3][4]. Recently, by using bioassay-guided fractionation, we identified a novel α-isocubebenol from the fruits of S. chinensis; this α-iso-cubebenol markedly inhibited lipopolysaccharide (LPS)-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in mouse macrophage RAW264.7 cells [5]. However, the anti-inflammatory signaling pathways involved in the α-iso-cubebenol-induced activation of heme oxygenase-1 (HO-1) in periodontitis and chronic inflammation are not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Schisandra chinensis α-iso-cubebenol induces heme oxygenase-1 expression through PI3K/Akt and Nrf2 signaling and has anti-inflammatory activity in Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages

Park

Kim

et al. 2011

International Immunopharmacology

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Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis

Cited by 28 publications

References 23 publications

Mystroxylon aethiopicum chloroform root bark extracts phytochemical analysis using gas chromatography mass spectrometry

Mystroxylon aethiopicum chloroform root bark extracts phytochemical analysis using gas chromatography mass spectrometry

The α-iso-cubebenol compound isolated from Schisandra chinensis induces p53-independent pathway-mediated apoptosis in hepatocellular carcinoma cells

Schisandra chinensis α-iso-cubebenol induces heme oxygenase-1 expression through PI3K/Akt and Nrf2 signaling and has anti-inflammatory activity in Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages

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