Identification of a RAPD marker linked to the oat stem rust gene Pg3

Penner, G.B.; Chong, J.; Lévesque-Lemay, Madeleine; Molnar, Stephen J.; Fedak, George

doi:10.1007/bf00225008

Cited by 73 publications

(23 citation statements)

References 11 publications

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“…RAPD analysis was based on methods reported by Fennimore et al (1999), Penner et al (1993) and Williams et al (1991). Polymerase chain reaction (PCR) conditions were optimised for repeatable amplification of oat and wild red oat DNA.…”

Section: Methodsmentioning

confidence: 99%

“…DNA markers and quantitative trait loci (QTLs) associated with anther culture ability traits have been reported in wheat, barley, maize and rice (Beaumont et al 1995, Murigneux et al 1994, He et al 1998, Yamagishi et al 1998, Manninen 2000, Torp et al 2001, Kwon et al 2002. In hexaploid oats, DNA markers associated with agronomically important traits, such as disease resistance (Penner et al 1993, O'Donoughue et al 1996, Bush and Wise 1998, Jin et al 1998, quality (Holland et al 1997, Kianian et al 2000, Groh et al 2001) and dwarfness (Milach et al 1997) have already been reported. However, markers associated with anther culture traits of oats have not yet been published.…”

Section: Introductionmentioning

confidence: 99%

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Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

Kiviharju¹,

Laurila²,

Lehtonen³

2008

AFSci

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A study was carried out to improve anther culture ability of the non-responsive cultivated oat, Avena sativa L. cv. Puhti by introgressing favourable alleles from the responsive wild red oat, Avena sterilis L. acc. CAV 2648. Anther culture ability of these parental lines and F 2 progenies of their cross and two backcrosses was tested. Genotype effects were significant on all anther culture traits measured. The number of anther culture derived embryo-like structures was highest in acc. CAV 2648, and the number of green regenerants from the Puhti × CAV 2648 progeny. Anther culture response was greatly reduced in backcross progeny and was least in cv. Puhti. Random amplified polymorphic DNA (RAPD) was used to test for marker associations with oat anther culture traits in a population of 38 F 2 progenies. Two RAPD markers were putatively associated with improved production of green regenerants (one derived from acc. CAV 2648 and the other from cv. Puhti). One marker putatively associated with decreased albino plant regeneration (derived from acc. CAV 2648). These markers might be useful for selecting alleles for better anther culture ability among progeny of planned crosses. In addition, three markers, derived from acc. CAV 2648, were putatively associated with decreased anther culture response rates.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

Kiviharju¹,

Laurila²,

Lehtonen³

2008

AFSci

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“…Since the development of PCR-based RAPD markers, in which random fragments of DNA are amplified from DNA samples using short, arbitrary primers [35], several resistance genes have been tagged with RAPD markers [e.g. 24,25,31,36]. The use of near-isogenic lines (NILs) can accelerate the identification of DNA markers tightly linked to genes of interest [e.g.…”

Section: Introductionmentioning

confidence: 99%

Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers

et al. 1995

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Four genes of rice, Oryza sativa L., conditioning resistance to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae), were tagged by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. No recombinants were observed between xa-5 and RFLP marker loci RZ390, RG556 or RG207 on chromosome 5. Xa-3 and Xa-4 were linked to RFLP locus XNpbl81 at the top of chromosome 11, at distances of 2.3 cM and 1.7 cM, respectively. The nearest marker to Xa-lO, also located on chromosome 11, was the RAPD locus 0072o00 at a distance of 5.3 cM. From this study, the conventional map [19,28] and two RFLP linkage maps of chromosome 11 [14,26] were partially integrated. Using the RFLP and RAPD markers linked to the resistance genes, we selected rice lines homozygous for pairs of resistance genes, Xa-4 + xa-5 and Xa-4 + Xa-lO. Lines carrying Xa-4 + xa-5 and Xa-4 + Xa-lO were evaluated for reaction to eight strains of the bacterial blight pathogen, representing eight pathotypes and three genetic lineages. As expected, the lines carrying pairs &genes were resistant to more of the isolates than their single-gene parental lines. Lines carrying Xa-4 + xa-5 were more resistant to isolates of race 4 than were either of the parental lines ('quantitative complementation'). No such effects were seen for Xa-4 + Xa-lO. Thus, combinations of resistance genes provide broader spectra of resistance through both ordinary gene action expected and quantitative complementation.

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“…The combination of near-isogenic lines and RAPD analysis was used effectively in tomato (Martin et al 1991), lettuce (Paran et al 1991), oat (Penner et al 1993), common bean (Haley et al 1993) and wheat (Dweikat et al 1994) for the purpose of identifying DNA markers for disease and insect resistance genes. However, markerassisted selection with RAPDs is not always feasible because RAPD markers identified in one population are not always useful for another, and some markers lack reliability.…”

Section: Introductionmentioning

confidence: 99%

Development of STS markers linked to Hessian fly resistance gene H6 in wheat

2002

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Hessian fly is one of the world's most destructive insect pests of wheat Triticum aestivum L. We have used the combination of near-isogenic lines (NIL) and random amplified polymorphic DNA (RAPD) analysis to screen up to 2,000 primers to identify DNA markers that are linked to gene H6 that confers resistance to biotype B of the insect. This screen produced six primers that show polymorphic fragments associated with resistance by H6. We have screened 440 F(2) individuals from a cross of the susceptible cultivar Newton and a NIL that contains H6 to verify the linkage between these markers and the resistance gene. A high-resolution genetic map was constructed based on recombination frequency. Two of the markers were tightly linked to the gene with no recombination observed, three were within 2.0 cM, and one was 11 cM from the gene. Three of the six markers were successfully converted to sequence tagged site (STS) markers. Both RAPD and STS primers were used to screen for the presence or absence of the resistance gene in wheat varieties. The identification of markers and construction of the genetic high resolution map provide the first steps toward localization of this resistance gene.

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Identification of a RAPD marker linked to the oat stem rust gene Pg3

Cited by 73 publications

References 11 publications

Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers

Development of STS markers linked to Hessian fly resistance gene H6 in wheat

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