Identification of active compounds in Ophiopogonis Radix from different geographical origins by UPLC-Q/TOF-MS combined with GC-MS approaches

Abstract

Ophiopogonis Radix, also known as Maidong in Chinese, is largely produced in the Sichuan and Zhejiang provinces: “Chuan-maidong (CMD)” and “Zhe-maidong (ZMD),” respectively. This study aimed to distinguish and evaluate the quality of CMD and ZMD. In this study, the tubers of CMD and ZMD were investigated using UPLC-Q/TOF-MS, GC-MS, and LC-MS methods, respectively. Overall, steroidal saponins, homoisoflavonoids, amino acids, and nucleosides were quickly identified. Furthermore, multivariate statistical analysis revealed that CMD and ZMD could be separated. Moreover, CMD showed higher levels of 4-aminobutanoic acid, glycine, l-proline, monoethanolamine, and serine than ZMD. Besides, the levels of chlorogenic acid, traumatic acid, cytidine, cadaverine, pyridoxine 5-phosphate, glutinone, and pelargonidin 3-O-(6-O-malonyl-β-d-glucoside) were remarkably higher in ZMD than in CMD. Furthermore, these different constituents were mainly associated with galactose metabolism; starch and sucrose metabolism; cysteine and methionine metabolism; valine, leucine, and isoleucine biosynthesis; and glycerophospholipid metabolism. In general, these results showed many differences between the bioactive chemical constituents of Ophiopogon japonicus from different production areas, where ZMD performed better in the quality assessment than CMD, and that UPLC-Q/TOF-MS, GC-MS, and LC-MS are effective methods to discriminate medicinal herbs from different production areas.

Characterization and Discrimination of Ophiopogonis Radix with Different Levels of Sulfur Fumigation Based on UPLC-QTOF-MS Combined Molecular Networking with Multivariate Statistical Analysis

“…With the aid of GNPS, a total of 28 steroidal saponins (9 disaccharide glycosides, 10 trisaccharide glycosides, 5 tetraglycoside and 4 pentaglycoside) were ambiguously or tentatively identified by comparing with the literature [ 40 , 41 , 42 ]. In addition, it is notable that there were some unidentified compounds in the BPI chromatogram and further investigation was necessary.…”

A Chinese classical prescription Maimendong decoction in treatment of pulmonary fibrosis: an overview

“…Modern pharmacological studies have discovered various beneficial effects of ophiopogonis , including anti-heart failure and anti-tumor properties, regulation of gastrointestinal smooth muscle and intestinal flora, protection of the nervous system, improvement of liver and lung injuries, anti-inflammatory and antioxidant effects, hypoglycemic and anti-aging properties, as well as immune regulation ( Shen et al, 2020 ). The main chemical components of ophiopogonis include steroidal saponins, polysaccharides, amino acids, and high isoflavone compounds ( Wang et al, 2021 ; Zha et al, 2022 ; Lei et al, 2024 ). Additionally, ophiopogonis and its extracts have demonstrated protective effects against lung injury ( Wang et al, 2020 ).…”

Xuanfudaizhe Decoction Alleviate Reflux Esophagitis through Inhibition of NLRP3/Caspase-1 Pathway

“…The active ingredients of XFDZ were detected using the ultra-performance liquid chromatography/quadrupole time-of-ight tandem mass spectrometry (UPLC/Q-TOF-MS). UPLC/Q-TOF-MS method was adapted from previously established method [15].…”