Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germ plasm by C-banding and in situ hybridization

Triebe, B.; Mukai, Yasuhiko; Dhaliwal, H. S.; Martin, Taylor; Gill, Bikram S.

doi:10.1007/bf00228680

Cited by 149 publications

(78 citation statements)

References 33 publications

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“…However, modern breeding was challenged by the narrow genetic variation [1,2], which affected further improvements in wheat yield and quality. Wild relatives of wheat possessed many desirable and valuable traits that could be used as gene resources for wheat improvement [3][4][5]. For example, Agropyron cristatum chromosome 6P with the genes controlling large numbers of florets and kernels per spike and multiple fertile tiller numbers per plant [6,7]; Dasypyrum villosum chromosome 1V including the seed storage…”

Section: Introductionmentioning

confidence: 99%

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Luan

Wang

Liu

et al. 2010

Planta

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Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable traits that can be transferred to common wheat through breeding programs. The wheat-A. cristatum disomic addition and translocation lines can be used as bridge materials to introduce alien chromosomal segments to wheat. Wheat-A. cristatum 2P disomic addition line II-9-3 was highly resistant to powdery mildew and leaf rust, which was reported in our previous study. However, some translocation lines induced from II-9-3 have not been reported. In this study, some translocation lines were induced from II-9-3 by 60 Co-γ irradiation and gametocidal chromosome 2C and then identified by cytological methods. Forty-nine wheat-A. cristatum translocation lines were obtained and various translcoation types were identified by GISH (genomic in situ hybridization), such as whole-arm, segmental and intercalary translocations. Dual-color FISH (fluorescent in situ hybridization) was applied to identify the wheat chromosomes involved in the translocations, and the results showed that A. cristatum 2P chromosome segments were translocated to the different wheat chromosomes, including 1A, 2A, 3A, 4A, 5A, 6A, 7A, 3B, 5B, 7B, 1D, 4D and 6D. Many different types of wheat-A. cristatum alien translocation lines would be valuable for not only identifying and cloning A. cristatum 2P-related genes and understanding the genetics and breeding effects of the translocation between A. cristatum chromosome 2P and wheat chromosomes, but also providing new germplasm resources for the wheat genetic improvement.

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Section: Introductionmentioning

confidence: 99%

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Luan

Wang

Liu

et al. 2010

Planta

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“…Fluorescence in situ hybridization (FISH) is well established as a highly efficient method for cytological characterization. It has been used for physical mapping of specific sequences in wheat and its relatives (Mukai et al, 1990 and1992;Leitch et al, 1991; to locate the breakpoints of chromosome translocations (Friebe et al, 1991;Mukai et al, 1993;, Molnar-Lang et al, 2000 and in karyotyping (Cabrera et al, 1995;Tsujimoto et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences

et al. 2005

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Hordeum vulgare , cultivated barley, and its wild relative, H. chilense, have several important traits that might be useful for wheat improvement. Here, in situ hybridization and barley expressed sequence tag (EST) markers were used to characterize and compare the chromosomes of H. chilense with those of H. vulgare . FISH with four repetitive DNA sequences, AG, AAG, 5S rDNA and 45S rDNA, was applied to the mitotic chromosomes of H. vulgare , H. chilense and available wheat-H. chilense addition and substitution lines. FISH with the AAG repeat differentiated the individual chromosomes of H. chilense and H. vulgare. The patterns of FISH signals in the two species differed greatly. The 45S rDNA signals were observed on two pairs of chromosomes in both species, while the 5S rDNA signals were observed on four pairs of chromosomes in H. vulgare and on one pair in H. chilense. The AG repeat showed FISH signals at the centromeric regions of all chromosomes of H. vulgare but none of the chromosomes of H. chilense . These results indicate that the chromosomes of the two species are highly differentiated. To study the homoeology between the two species, 209 EST markers of H. vulgare were allocated to individual chromosomes of H. chilense. One hundred and forty of the EST markers were allocated to respective chromosomes of H. chilense using the wheat-H. chilense addition and substitution lines. Twenty-six EST markers on average were allocated to each chromosome except to the chromosome 2H ch S, to which only 10 markers were allocated. Ninety percent of the allocated EST markers in H. chilense were placed on H. vulgare chromosomes of the same homoeologous group, indicating that the expressed sequences of the two species were highly conserved. These EST markers would be useful for detecting chromatin introgressed from these species into the wheat genome.

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“…Genetic resistance to WSMV offers the most environmentally and economically desirable strategy for disease control and has been pursued since resistance to WSMV was discovered (Friebe et al, 1991;Graybosch et al, 2009;Mutti et al, 2011). Today, there is huge interest in both using genomic prediction to access genotype × environment interaction using marker and pedigree information in breeding programmes (Crossa et al, 2014), and in enhancement of the genetic variability available in wheat through wide hybridisation (see Heslop-Harrison and Schwarzacher, 2012).…”

Section: Introductionmentioning

confidence: 99%

Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

et al. 2016

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Pyramiding of alien-derived Wheat streak mosaic virus (WSMV) resistance and resistance enhancing genes in wheat is a costeffective and environmentally safe strategy for disease control. PCR-based markers and cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin in four genetically diverse populations of wheat (Triticum aestivum) lines incorporating chromosome segments from Thinopyrum intermedium and Secale cereale (rye). Out of 20 experimental lines, 10 carried Th. intermedium chromatin as T4DL*4Ai#2S translocations, while, unexpectedly, 7 lines were positive for alien chromatin (Th. intermedium or rye) on chromosome 1B. The newly described rye 1RS chromatin, transmitted from early in the pedigree, was associated with enhanced WSMV resistance. Under field conditions, the 1RS chromatin alone showed some resistance, while together with the Th. intermedium 4Ai#2S offered superior resistance to that demonstrated by the known resistant cultivar Mace. Most alien wheat lines carry whole chromosome arms, and it is notable that these lines showed intra-arm recombination within the 1BS arm. The translocation breakpoints between 1BS and alien chromatin fell in three categories: (i) at or near to the centromere, (ii) intercalary between markers UL-Thin5 and Xgwm1130 and (iii) towards the telomere between Xgwm0911 and Xbarc194. Labelled genomic Th. intermedium DNA hybridised to the rye 1RS chromatin under high stringency conditions, indicating the presence of shared tandem repeats among the cereals. The novel small alien fragments may explain the difficulty in developing well-adapted lines carrying Wsm1 despite improved tolerance to the virus. The results will facilitate directed chromosome engineering producing agronomically desirable WSMV-resistant germplasm.

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Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germ plasm by C-banding and in situ hybridization

Cited by 149 publications

References 33 publications

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences

Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

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