Identification of an exosomal long non-coding RNAs panel for predicting recurrence risk in patients with colorectal cancer

Liu, Hui; Liu, Xinfeng; Guo, Yulian; Wang, Yanlei; Dai, Yonggang; Zhuo, Jinhua; Wu, Bing; Wang, Hongchun; Zhang, Xin

doi:10.18632/aging.103006

Cited by 9 publications

(5 citation statements)

References 50 publications

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“…The medium of M2 macrophages promoted the osteogenic differentiation of stem cells compared to the control group [ 20 ], suggesting the M2 macrophge may regulate MSCs property via paracine pathway. Exosomes play a critical role in intercellular communication by transferring bioactive molecules between cells, such as lipids, proteins, and genetic information [ 28 , 29 ]. We found that the exosomes of M2 macrophages could induce osteogenic differentiation of MSCs, which is consistent with the previous study that exosomes of M2 macrophages induced MSCs to differentiate towards osteoblasts by targeting salt-inducible kinase 2 and 3 [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

M2 exosomes modified by hydrogen sulfide promoted bone regeneration by moesin mediated endocytosis

Zhou

Han

Zhu

et al. 2024

Bioactive Materials

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Section: Discussionmentioning

confidence: 99%

M2 exosomes modified by hydrogen sulfide promoted bone regeneration by moesin mediated endocytosis

Zhou

Han

Zhu

et al. 2024

Bioactive Materials

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“…Our previous study has reported that several long noncoding RNAs in exosomes of serum are associated their expression in tumor tissues (35). To better understand whether circulating exosomal Cripto-1 is represented in tissue biopsies, Cripto-1 expression was further examined in the tissues of PHCAA patients.…”

Section: Discussionmentioning

confidence: 99%

Exosomal Cripto-1 Serves as a Potential Biomarker for Perihilar Cholangiocarcinoma

Zhang

et al. 2021

Front. Oncol.

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Perihilar cholangiocarcinoma (PHCCA) has a poor prognosis, mainly due to diagnosis at an advanced stage. Cripto-1 functions as an oncogene and is highly expressed in several human cancers, however, its clinical application in PHCCA is poorly understood. Herein, we identified that Cripto-1 was released by PHCCA cells via exosomes in vitro and in vivo. Furthermore, an ELISA method was developed to detect exosomal Cripto-1 in the serum of 115 PHCCA patients, 47 cholangitis patients and 65 healthy controls, and it was found that exosomal Cripto-1 was increased in PHCCA patients and associated with metastasis. Compared with traditional serum tumor markers, CA19-9 and CEA, exosomal Cripto-1 demonstrated a larger area under ROC curve for PHCCA diagnosis. The cutoff value of exosomal Cripto-1 was 0.82, achieving a sensitivity of 79.1% and a specificity of 87.5%. As expected, exosomal Cripto-1 levels in immunohistochemically Cripto-1-high cases were significantly elevated compared to in Cripto-1-low cases. When measured 1-week postoperatively, Cripto-1 levels decreased on average from 1.25(0.96-3.26) to 0.85(0.62-1.82). Immunohistochemistry analysis showed Cripto-1 expression was negatively correlated with E-cadherin and was an independent prognostic biomarker for poor survival in PHCCA patients. In conclusion, exosomal Cripto-1 in sera can reflect its expression in the tissue of PHCAA patients and has the potential be a non-invasive biomarker for diagnosis and prognosis of PHCCA.

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“…A growing number of researches have clarified that lncRNAs involve in a variety of cellular biological functions, including chromatin imprinting, cell differentiation, tumorigenesis, and cancer cell drug resistance [ 9 , 10 ]. Importantly, lncRNAs as promising candidates for the diagnosis of CRC have been discovered [ 11 – 14 ]. Despite great efforts in understanding the occurrence and progression of CRC, the clinical trials of CRC treatments had disappointing results.…”

Section: Introductionmentioning

confidence: 99%

LINC01116 facilitates colorectal cancer cell proliferation and angiogenesis through targeting EZH2-regulated TPM1

Liang

Qiu

2021

J Transl Med

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Background Colorectal cancer (CRC) is a common malignant tumor globally. Meanwhile, LINC01116 has been proposed as risk factor for various tumors, including CRC. But the regulation of LINC01116 in CRC required more validated data. This study aimed to elucidate the potential function of LINC01116 in regulating cell proliferation and angiogenesis of CRC. Methods LINC01116 expression in 80 pairs of CRC tumor and adjacent non-tumor tissues was determined by qRT-PCR. After transfection of pcDNA3.1-LINC01116, sh-LINC01116, sh-TPM1, pcDNA3.1-EZH2 or sh-EZH2 in SW480 and HCT116 cells, the levels of LINC01116, TPM1 and EZH2 were measured by qRT-PCR or Western blot. The cell biological function of CRC cell lines was determined by CCK-8, colony formation assays, tube formation and scratch assays. RNA pull-down and RIP assays were applied to detect the binding of LINC01116 with EZH2 and H3K27me3. Binding of EZH2 to the TPM1 promoter was assessed by ChIP assay. Finally, xenograft models in nude mice were established to validate the results of in vitro experiments. Results LINC01116 was overexpressed in CRC tissues and high expression of LINC01116 was negatively correlated with postoperative survival. In vitro study showed LINC01116 expression could significantly enhance CRC progression, including increasing cell proliferation, migration and angiogenesis. Besides, investigations into the mechanism disclosed that LINC01116 could regulate EZH2 to inactivate TPM1 promoter, thus promoting CRC cell proliferation and angiogenesis. Moreover, consistent results of in vivo experiments were conformed in vitro experiments. Conclusion LINC01116 promotes the proliferation and angiogenesis of CRC cells by recruiting EZH2 to potentiate methylation in the TPM1 promoter region to inhibit the transcription of TPM1.

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Identification of an exosomal long non-coding RNAs panel for predicting recurrence risk in patients with colorectal cancer

Cited by 9 publications

References 50 publications

M2 exosomes modified by hydrogen sulfide promoted bone regeneration by moesin mediated endocytosis

M2 exosomes modified by hydrogen sulfide promoted bone regeneration by moesin mediated endocytosis

Exosomal Cripto-1 Serves as a Potential Biomarker for Perihilar Cholangiocarcinoma

LINC01116 facilitates colorectal cancer cell proliferation and angiogenesis through targeting EZH2-regulated TPM1

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