Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis

Sonesson, Andreas; Przybyszewska, Kornelia; Eriksson, Sigrid; Mörgelin, Matthias; Kjellström, Sven; Davies, Julia R.; Potempa, Jan; Schmidtchen, Artur

doi:10.1038/s41598-017-08046-2

Cited by 86 publications

(82 citation statements)

References 68 publications

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“…In patients with atopic dermatitis, S. aureus isolates grow as biofilms on the skin and produce proteases that degrade host AMPs, such as cathelicidin LL-37 91 . The host has evolved mechanisms to ward off invasion by S. aureus at every level of the skin and subcutaneous tissue.…”

Section: Host–pathogen Interactionsmentioning

confidence: 99%

Skin microbiota–host interactions

Chen

Fischbach

Belkaid

2018

Nature

540

396

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The skin is a complex and dynamic ecosystem that is inhabited by bacteria, archaea, fungi and viruses. These microbes—collectively referred to as the skin microbiota—are fundamental to skin physiology and immunity. Interactions between skin microbes and the host can fall anywhere along the continuum between mutualism and pathogenicity. In this Review, we highlight how host–microbe interactions depend heavily on context, including the state of immune activation, host genetic predisposition, barrier status, microbe localization, and microbe–microbe interactions. We focus on how context shapes the complex dialogue between skin microbes and the host, and the consequences of this dialogue for health and disease.

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Section: Host–pathogen Interactionsmentioning

confidence: 99%

Skin microbiota–host interactions

Chen

Fischbach

Belkaid

2018

Nature

540

396

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“…In comparison to MIC determination, these assays are often conducted using flat bottom or modified 96-well plates, called the Calgary device or high-throughput plates (the schematic diagram of both methods is presented in Figure 2) [74,75]. Both assays enable the determination of the minimum biofilm eradication concentration (MBEC) [76,77].…”

Section: Minimal Biofilm Eradication/eliminating Concentration Assaysmentioning

confidence: 99%

Methods Used for the Eradication of Staphylococcal Biofilms

et al. 2019

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Staphylococcus aureus is considered one of the leading pathogens responsible for community and healthcare-associated infections. Among them, infections caused by methicillin-resistant strains (MRSA) are connected with ineffective or prolonged treatment. The therapy of staphylococcal infections faces many difficulties, not only because of the bacteria’s resistance to antibiotics and the multiplicity of virulence factors it produces, but also due to its ability to form a biofilm. The present review focuses on several approaches used for the assessment of staphylococcal biofilm eradication. The methods described here are successfully applied in research on the prevention of biofilm-associated infections, as well as in their management. They include not only the evaluation of the antimicrobial activity of novel compounds, but also the methods for biomaterial functionalization. Moreover, the advantages and limitations of different dyes and techniques used for biofilm characterization are discussed. Therefore, this review may be helpful for those scientists who work on the development of new antistaphylococcal compounds.

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“…Thus, mutations in this gene can disrupt the structure of the skin barrier, resulting in increased permeability to allergens . Interestingly, skin barrier impairment correlates with infections from S. aureus , a main player in AD, that produces toxins such as enterotoxins which can act like superantigens . Activated MCs and eosinophils are found in AD lesions and both can be activated by S. aureus directly via TLRs or CD48 .…”

Section: Mast Cells and Eosinophils In Allergic Diseasementioning

confidence: 99%

“…226 Interestingly, skin barrier impairment correlates with infections from S. aureus, 227 a main player in AD, that produces toxins such as enterotoxins which can act like superantigens. 224,228,229 Activated…”

Section: Atopic Dermatitismentioning

confidence: 99%

Human eosinophils and mast cells: Birds of a feather flock together

Robida

Puzzovio

Pahima

et al. 2018

Immunological Reviews

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While the origin of the phrase "birds of a feather flock together" is unclear, it has been in use for centuries and is typically employed to describe the phenomenon that people with similar tastes or interests tend to seek each other out and congregate together. In this review, we have co-opted this phrase to compare innate immune cells of related origin, the eosinophil and mast cell, because they very often accumulate together in tissue sites under both homeostatic and inflammatory conditions. To highlight overlapping yet distinct features, their hematopoietic development, cell surface phenotype, mediator release profiles and roles in diseases have been compared and contrasted. What emerges is a sense that these two cell types often interact with each other and their tissue environment to provide synergistic contributions to a variety of normal and pathologic immune responses.

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Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis

Cited by 86 publications

References 68 publications

Skin microbiota–host interactions

Skin microbiota–host interactions

Methods Used for the Eradication of Staphylococcal Biofilms

Human eosinophils and mast cells: Birds of a feather flock together

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