Identification of benzimidazole resistance in Cladobotryum dendroides using a PCR-based method

McKay, Gareth; Egan, Damian; Morris, Elizabeth; Brown, Averil E.

doi:10.1017/s095375629700542x

Cited by 69 publications

(51 citation statements)

References 11 publications

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“…[23] Contrary to our results, a majority of Cladobotryum isolates from the Republic of Ireland and the UK were found to be resistant to MBC fungicides after outbreaks of cobweb disease that reached epidemic proportions in 1994/5. [3,10] The Irish isolates were tolerant to benomyl and carbendazim at concentrations up to 10.00 mg L −1 . [3] At the height of cobweb epidemic in the UK in 1995, 25% of the isolates were weakly resistant to thiabendazole (EC 50 values ranging between 1.00 and 10.00 mg L −1 ) and sensitive to carbendazim (EC 50 < 1.00 mg L −1 ), while the remaining 75% were strongly resistant to thiabendazole (EC 50 > 200.00 mg L −1 ) and weakly resistant to carbendazim (EC 50 values ranged between 2.00 and 10.00 mg L −1 ).…”

Section: Antifungal Activity Of Different Fungicidesmentioning

confidence: 99%

“…[3,10] The Irish isolates were tolerant to benomyl and carbendazim at concentrations up to 10.00 mg L −1 . [3] At the height of cobweb epidemic in the UK in 1995, 25% of the isolates were weakly resistant to thiabendazole (EC 50 values ranging between 1.00 and 10.00 mg L −1 ) and sensitive to carbendazim (EC 50 < 1.00 mg L −1 ), while the remaining 75% were strongly resistant to thiabendazole (EC 50 > 200.00 mg L −1 ) and weakly resistant to carbendazim (EC 50 values ranged between 2.00 and 10.00 mg L −1 ). [10] Thiabendazole was not included in our study as it is not commonly used in Serbian mushroom farms.…”

Section: Antifungal Activity Of Different Fungicidesmentioning

confidence: 99%

“…[28] A mutation point was detected on the β-tubulin gene in C. dendroides isolates resistant to benzimidazole fungicides, causing amino acid substitution from tyrosine to cysteine. [3] Sensitivity tests of the Serbian C. dendroides isolates have shown that although they were weakly resistant to thiophanate-methyl, cross resistance was observed neither to benomyl nor carbendazim. Cross resistance to MBC fungicides had been detected in the mushroom pathogen Verticillium fungicola, which was found highly resistant to thiabendazole and benomyl.…”

Section: Antifungal Activity Of Different Fungicidesmentioning

confidence: 99%

“…[1,2] The disease symptoms are cottony fluffy white or yellowish to pink colonies on mushroom casing, rapid colonization of casing surface, covering of host basidiomata by mycelia, and their decay. [3] Cobweb disease is currently one of the most serious diseases in Serbian mushroom farms affecting product quality and yield. [4] At the beginning of usage of fungicides from the group of methyl benzimidazole carbamates (MBC) in the late 1960s, excellent control of many fungal diseases was noted.…”

Section: Introductionmentioning

confidence: 99%

“…[8] The resistance of cobweb disease agents to MBC fungicides, especially to benomyl and carbendazim, was reported after their extensive usage in the Republic of Ireland in 1992. [3,9] In 1994/95, when cobweb disease took epidemic proportions in British mushroom farms, the majority of Cladobotryum spp. isolates were strongly resistant to thiabendazole and weakly resistant to carbendazim.…”

Section: Introductionmentioning

confidence: 99%

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In vitrotoxicity of selected fungicides from the groups of benzimidazoles and demethylation inhibitors toCladobotryum dendroidesandAgaricus bisporus

Potočnik

Vukojević

Stajić

et al. 2009

Journal of Environmental Science and Health, Part B

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Twenty microfungal isolates were collected from diseased fruiting bodies of Agaricus bisporus sampled from Serbian mushroom farms during [2003][2004][2005][2006][2007]. Based on morphological characteristics and pathogenicity tests, the isolates were identified as Cladobotryum dendroides. The isolates of C. dendroides and A. bisporus F56 and U3 were tested for sensitivity to several selected fungicides in vitro. C. dendroides isolates were found to be more sensitive to prochloraz manganese and flusilazole + carbendazim than to the other fungicides tested (EC 50 values were 0.09 and 0.11 mg L −1 , respectively) and weakly resistant to thiophanate-methyl (EC 50 values ranged between 6.53 and 12.09 mg L −1 ). Selectivity indexes of the tested fungicides on both C. dendroides and A. bisporus indicated that thiophanate-methyl, cyproconazole + carbendazim and flusilazole + carbendazim had much less selective fungitoxicity than benomyl, carbendazim and prochloraz manganese.

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Section: Antifungal Activity Of Different Fungicidesmentioning

confidence: 99%

Section: Antifungal Activity Of Different Fungicidesmentioning

confidence: 99%

Section: Antifungal Activity Of Different Fungicidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vitrotoxicity of selected fungicides from the groups of benzimidazoles and demethylation inhibitors toCladobotryum dendroidesandAgaricus bisporus

Potočnik

Vukojević

Stajić

et al. 2009

Journal of Environmental Science and Health, Part B

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Fungicide control of mushroom cobweb disease caused by Cladobotryum strains with different benzimidazole resistance profiles

Grogan

2006

Pest Management Science

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The benzimidazole fungicides thiabendazole and carbendazim, and the imidazole fungicide prochloraz-Mn, were tested for their efficacy in controlling cobweb disease of mushrooms caused by two Cladobotryum isolates. Isolate 202A was benzimidazole-sensitive in vitro and cobweb growth on the casing was well controlled by both benzimidazole fungicides in cropping experiments. Carbendazim also controlled the development of spotting symptoms much more effectively than thiabendazole. A second isolate (192B1) was benzimidazole-resistant and was highly resistant to thiabendazole in vitro but it showed some sensitivity to carbendazim in vitro at moderate to high concentrations. Despite this, carbendazim did not control disease symptoms in cropping experiments, confirming that isolate 192B1 is cross-resistant to other benzimidazole fungicides. Both isolates showed some sensitivity to prochloraz-Mn in vitro. This fungicide gave between 45% and 65% control of cobweb growth on the casing caused by either 202A or 192B1 but gave no control of spotting symptoms. Reducing the fungicide application volume did not give enhanced disease control. The emergence of benzimidazole resistance reduces the value of benzimidazoles in the control of mushroom pathogens. However, the lack of effective alternatives means they continue to have utility in cases where pathogens are still sensitive but this requires regular monitoring of pathogen populations for resistance.

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Detection and molecular characterisation of Pyrenopeziza brassicae isolates resistant to methyl benzimidazole carbamates

Carter

Cools

West

et al. 2013

Pest Management Science

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Identification of benzimidazole resistance in Cladobotryum dendroides using a PCR-based method

Cited by 69 publications

References 11 publications

In vitrotoxicity of selected fungicides from the groups of benzimidazoles and demethylation inhibitors toCladobotryum dendroidesandAgaricus bisporus

In vitrotoxicity of selected fungicides from the groups of benzimidazoles and demethylation inhibitors toCladobotryum dendroidesandAgaricus bisporus

Fungicide control of mushroom cobweb disease caused by Cladobotryum strains with different benzimidazole resistance profiles

Detection and molecular characterisation of Pyrenopeziza brassicae isolates resistant to methyl benzimidazole carbamates

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