Identification of Cancer Stem-like CD44+ Cells in Human Nasopharyngeal Carcinoma Cell Line

Xu, Xiaogang; Huang, Qiao; Lu, Mengji; Li, Daojun; Xue, Feng; Fang, Yi; Ren, Jinghua; Wu, Yue

doi:10.1016/j.arcmed.2011.01.007

Cited by 69 publications

(72 citation statements)

References 20 publications

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“…The stem cell surface protein CD44 was characterized as a family of cell surface proteoglycans and glycoproteins involved in tumor invasion, metastasis, chemo-and radiotherapy resistance (Wu et al, 2007). Recent studies in nasopharyngeal carcinoma also showed that increased expression of CD44 is responsible for tumorigenesis and metastasis (Su et al, 2011). In line with these findings, we also observed SP cells are highly immuno positive to CD44 when compared to non-SP cells.…”

Section: Discussionsupporting

confidence: 79%

Phenotypic characterization of drug resistance and tumor initiating cancer stem cells from human bone tumor osteosarcoma cell line OS-77

Zhang

Quan²,

Cheng

et al. 2014

Bangladesh J Pharmacol

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Section: Discussionsupporting

confidence: 79%

Phenotypic characterization of drug resistance and tumor initiating cancer stem cells from human bone tumor osteosarcoma cell line OS-77

Zhang

Quan²,

Cheng

et al. 2014

Bangladesh J Pharmacol

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“…All of the staining techniques described above are also suitable for the isolation of CSCs by flow cytometry (66,142,143). Two main sorting principles exist, which are designated as electrostatic sorting (144) and mechanical sorting (145).…”

Section: Sorting Of Cscsmentioning

confidence: 99%

Flow cytometry in cancer stem cell analysis and separation

et al. 2012

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In recent years, a special type of cancer cell-the cancer stem cell (CSC)-has been identified and characterized for different tumors. CSCs may be responsible for the recurrence of a tumor following a primarily successful therapy and are thought to bear a high metastatic potential. For the development of efficient treatment strategies, the establishment of reliable methods for the identification and effective isolation of CSCs is imperative. Similar to their stem cell counterparts in bone marrow or small intestine, different cluster of differentiation surface antigens have been characterized, thus enabling researchers to identify them within the tumor bulk and to determine their degree of differentiation. In addition, functional properties characteristic of stem cells can be measured. Side population analysis is based on the stem cell-specific activity of certain ATP-binding cassette transporter proteins, which are able to transport fluorescent dyes out of the cells. Furthermore, the stem cell-specific presence of aldehyde dehydrogenase isoform 1 can be used for CSC labeling. However, the flow cytometric analysis of these CSC functional features requires specific technical adjustments. This review focuses on the principles and strategies of the flow cytometric analysis of CSCs and provides an overview of current protocols as well as technical requirements and pitfalls. A special focus is set on side population analysis and analysis of ALDH activity. Flow cytometrybased sorting principles and future flow cytometric applications for CSC analysis are also discussed. ' 2012 International Society for Advancement of Cytometry

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“…CD44 is a member of a family of cell surface proteoglycans and glycoproteins, which have roles in tumor invasion, metastasis and resistance to chemotherapy and radiotherapy (9,(21)(22)(23). A previous study that investigated nasopharyngeal carcinoma demonstrated an increased expression of CD44-positive cells that may have initiated increased rates of tumorigenesis and metastasis (11). In accordance with these findings, the present study revealed that the cancer stem-like SP cells exhibited an increased level of CD44 expression compared with the non-SP cells.…”

Section: Discussionmentioning

confidence: 99%

“…CSCs can be isolated based upon the expression of stem cell surface markers, such as cluster of differentiation (CD)44 and CD133 (11), or by the Hoechst 33342 dye exclusion technique by fluorescence-activated cell sorting (FACS) (12). With FACS, the cells that exclude Hoechst 33342 dye are named the side population (SP) cells.…”

Section: Introductionmentioning

confidence: 99%

Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development

Liu

Sun²,

Xiao

et al. 2015

Oncology Letters

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Abstract. Osteosarcoma (OS) is an aggressive primary bone cancer that usually affects children and young adolescents. Previous studies have demonstrated the implications of a small sub-population of cancer stem cells on treatment failure and tumor recurrence. The present study analyzed the characteristic features of the stem-like cells within the human OS-55 cell line. It was identified that 2.3% of the OS-55 cells were cancer stem-like side population (SP) cells. Following treatment with verapamil, the population of SP cells was reduced to 0.7%. The sphere formation assay revealed that the OS cells were able to rapidly form tumor spheres (also known as sarcospheres). Immunofluorescence analysis identified that the OS-55 cells expressed the cluster of differentiation 44, octamer-binding transcription factor-3/4A and Nanog stem cell surface markers. The results of the present study suggest that, as with other tumors, OS also contains a sub-population of cancer stem-like cells, which may have important implications in cancer diagnosis and treatment. IntroductionOsteosarcoma (OS) is the eighth most common malignancy in children and young adolescents, and accounts for 2.4% of all pediatric malignancies. Following diagnosis, the mean survival rate of patients with OS is less than five years (1,2). In total, ~20% of patients are at the metastatic lung stage at the time of diagnosis. Unsuccessful treatment strategies lead to mortality. Therefore, an understanding of the molecular mechanisms that underlie tumorigenesis is important.Previous studies concerning a number of different cancers have identified the existence of a small sub-population of cancer-initiating cells known as cancer stem cells (CSCs), which exhibit stem cell-like properties and are responsible for tumor metastasis, invasion and chemotherapy resistance (3-8).The existence of CSCs has also been demonstrated in human OS, which possesses characteristic features of stem cells and expresses the octamer-binding transcription factor (Oct)3/4A and Nanog stem cell surface markers (9,10). A noteworthy feature of these cells is their ability to efflux Hoechst 3342 dye and other chemotherapeutic drugs (10), which ultimately results in chemoresistance and tumor recurrence.CSCs can be isolated based upon the expression of stem cell surface markers, such as cluster of differentiation (CD)44 and CD133 (11), or by the Hoechst 33342 dye exclusion technique by fluorescence-activated cell sorting (FACS) (12). With FACS, the cells that exclude Hoechst 33342 dye are named the side population (SP) cells. Another technique is the sphere formation assay, in which individual cancer cells form a spherical colony in a defined serum-free medium (10). In each of these methods, the isolated cancer stem-like cells exhibit resistance to chemotherapy and apoptosis, a high level of self-renewal and a higher level of stem cell surface markers. However, the molecular and signaling pathways involved in these functions are yet to be elucidated. The present study attempted to isolate and ch...

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Identification of Cancer Stem-like CD44+ Cells in Human Nasopharyngeal Carcinoma Cell Line

Cited by 69 publications

References 20 publications

Phenotypic characterization of drug resistance and tumor initiating cancer stem cells from human bone tumor osteosarcoma cell line OS-77

Phenotypic characterization of drug resistance and tumor initiating cancer stem cells from human bone tumor osteosarcoma cell line OS-77

Flow cytometry in cancer stem cell analysis and separation

Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development

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