Identification of critical miRNAs and mRNAs associated with polycystic ovary syndrome

Abstract

Aim Polycystic ovary syndrome (PCOS) is a complicated endocrine and metabolic abnormality diseases common in women of child‐bearing age. This study aims to screen out critical miRNAs and mRNAs associated with PCOS, which may be conducive to offer novel insights and treatment for the diseases. Methods Three mRNA datasets and one miRNA dataset derived from granulosa cells of patients with PCOS and normal controls were downloaded to obtain the differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs). Then, DEmiRNA‐target DEmRNAs analysis and functional annotation of DEmiRNA‐target DEmRNAs were performed. Quantitative real time polymerase chain reaction (qRT‐PCR) validation of the expression of the selected DEmRNAs and DEmiRNAs were performed. Results A total of 1643 DEmRNAs, 88 DEmiRNAs, 2406 DEmiRNA (down)‐DEmRNA (up), and 2179 DEmiRNA (up)‐DEmRNA (down) pairs were obtained. The functional annotation of DEmiRNA‐target DEmRNAs revealed that C‐type lectin receptor signaling pathway, Steroid biosynthesis and Galactose metabolism were significantly enriched KEGG pathways. Conclusion These findings may provide make contribution to understanding PCOS pathogenesis, diagnosis, or treatment.

Treatment of D-galactose-induced rat polycystic ovarian condition using Lepidium sativum and secondary antibodies

“…The results showed that at the second and fourth weeks after induction, the calibrated concentration of undiluted samples in the D-galactose group was meaningfully higher than that of rat normal samples. According to a different bioinformatics study, PCOS has a significantly enriched D-galactose metabolism pathology [ 11 ].…”

“…They can also impede oocyte growth and decrease the bioactivity of FSH. D-galactose metabolism is a disease that is greatly enriched in PCOS, which is relevant to the relationship between D-galactose and PCOS [ 11 ]. The pathogenic mechanisms in premature ovarian failure include the generation of anti-ovarian antibodies (AOA) against ovarian antigens, infiltration of inflammatory cells, elevated levels of proinflammatory cytokines, and decreased Tregs [ 12 ].…”

Association of galactose and insulin resistance in polycystic ovary syndrome: A case-control study

“… 23 Another bioinformatics analysis has suggested that galactose metabolism is a significantly enriched pathway in PCOS. 24 To date, no study has investigated the circulating galactose levels in patients with PCOS. Our results clearly indicate higher baseline galactose levels in women with PCOS, which are correlated with PCOS itself, and independent of the metabolic status as well as other relevant covariates.…”