Identification of DNA methylation and transcriptional regulatory regions in the promoter of duck retinoic acid inducible gene I (RIG-I)

Abstract

1. The aim of this study was to identify the active control area of the duck retinoic acid-inducible gene I (duRIG-I) core promoter, to predict the binding sites of transcription factors and to provide a theoretical basis for the study of duRIG-I function and mechanism of regulation. 2. The promoter region of duRIG-I was obtained from Ensembl; the CpG island in the duRIG-I promoter was predicted online; and the methylation status of the duRIG-I promoter was detected by the bisulphite sequencing PCR method. 3. There was an obvious CpG island in the duRIG-I promoter, with a total of 44 CG dinucleotides. However, the level of methylation was hypomethylation (0.2%). 4. The core transcriptional regulatory region was identified, localised between -301 and ~+14 bp, where multiple transcription factor binding sites including IRF1, RXRβ, AP-2αA, RAP1, NF-1 and SP1 motifs were identified that significantly enhanced RIG-I promoter activity.

DNA methylation and single-nucleotide polymorphisms in DDX58 are associated with hand, foot and mouth disease caused by enterovirus 71

“…The CpG context is highly methylated, while most CpG islands are not methylated, which means that CpG islands in the promoter region can be methylated, resulting in the inhibition of gene expression [ 19 – 22 ]. The gene expression of RIG-I and MDA5 is also regulated by the methylation level of CpG islands in the promoter region of the coding genes [ 23 , 24 ]. The methylation level of CpG islands can reflect the expression of RIG-I and MDA5 and indirectly reflect the antiviral effect [ 23 ].…”

“…The gene expression of RIG-I and MDA5 is also regulated by the methylation level of CpG islands in the promoter region of the coding genes [ 23 , 24 ]. The methylation level of CpG islands can reflect the expression of RIG-I and MDA5 and indirectly reflect the antiviral effect [ 23 ]. Zhang et al found that the methylation level of the chicken genome was significantly upregulated after infection with H5N1 avian influenza virus [ 25 ].…”