Identification of environmental Vibrio vulnificus isolates with a DNA probe for the cytotoxin-hemolysin gene

Abstract

We screened 44 lactose-positive Vibrio strains isolated from the marine environment for homology with a 3.2-kilobase DNA fragment encoding the Vibrio vulnificus cytotoxin-hemolysin gene. All 29 marine isolates identified as V. vulnificus on the basis of numerical taxonomy and DNA-DNA hybridization studies hybridized with the cytotoxin gene probe, as did all V. vulnificus reference strains. Homologous gene sequences were identified in no other lactose-positive marine vibrio isolates nor in 10 other Vibrio species.

Light‐scattering sensor for real‐time identification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae colonies on solid agar plate

“…Colonies of V. parahaemolyticus were confirmed by using VPM1 and VPM2 primers specific for the metalloprotease gene (Luan et al, 2007). Vibrio vulnificus was confirmed by using Cyt1 and Cyt2 primers targeting the haemolysin/cytolysin gene (Morris et al, 1987). A standard amplification condition was used consisting of initial denaturation at 94°C for 2 min, followed by 30 cycles of 94°C for 1 min, 58°C for 1 min and 72°C for 1 min, and final extension at 72°C for 5 min.…”

Updated perspectives on emerging vibrios associated with human infections

“…PCR procedures targeting the gyrB gene, encoding the B subunit of DNA gyrase essential for DNA replication, and the regulatory toxR gene, were used for the specific detection of V. parahaemolyticus (Miller et al 1987;Lin et al 1993;Reich and Schoolnik 1994;Kim et al 1999). The FDA procedure includes the use of a gene probe for the identification of V. vulnificus, targeting cytotoxinhaemolysin, and a gene probe, targeting tdh gene for detecting of V. parahaemolyticus (Morris et al 1987;Yamamoto et al 1990). A rapid, specific enumeration technique for V. vulnificus, using a nonradioactive probe, is currently being evaluated (Wright et al 1993;FDA, 1998).…”

Comparison of a commercial biochemical kit and an oligonucleotide probe for identification of environmental isolates of Vibrio vulnificus

“…which is unique to this species (Wright et al 1985 ;Morris et al 1987). This gene has been sequenced (Yamamoto et al 1990) and used as the basis of an oligonucleotide probe for the identification of V. vulnzjicus (Wright et al 1993).…”