Identification of Fasciola spp. based on ITS-2 reveals the Fasciola gigantica infection in buffaloes in Nanning city, South China

Wu, Zhengjiao; Wang, Jinhui; Meng, Zhen; Jin, Weikun; He, Kangxin; Zhang, Weiyu; Di, Wenda

doi:10.1016/j.vetpar.2021.109585

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…Fasciolosis, caused by Fasciola hepatica (in temperate zones) and Fasciola gigantica (in tropical zones), is one of the most widespread ruminant parasitic diseases and causes significant economic loss in the husbandry sector ( 1 , 2 ). The tropical liver fluke, F. gigantica, affects the vitality and reproduction of infected buffaloes; given the high infection rate in buffaloes, this poses a serious threat to buffalo farming in Africa and Asia ( 3 , 4 ).…”

Section: Introductionmentioning

confidence: 99%

Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

et al. 2023

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BackgroundAs a natural host of Fasciola gigantica, buffalo is widely infected by F. gigantica. Its impact on buffalo production has caused great losses to the husbandry sector, and repeat infection is non-negligible. In buffaloes experimentally infected with F. gigantica, primary and secondary infection have yielded the same rate of fluke recovery, indicating a high susceptibility of buffalo to F. gigantica, which contributes to the high infection rate. Determining the immunological mechanism of susceptibility will deepen the understanding of the interaction between F. gigantica and buffalo. Here, we explored the immune response of buffaloes against primary and secondary F. gigantica infection, with a focus on cytokines’ dynamics explored through serum cytokine detection.MethodsBuffaloes were assigned to three groups: group A (noninfected, n = 4), group B (primary infection, n = 3), and group C (secondary infection, n = 3). Group B was infected via oral gavage with 250 viable F. gigantica metacercariae, and group C was infected twice with 250 metacercariae at an interval of 4 weeks. The second infection of group C was performed simultaneously with that of group B. Whole blood samples were collected pre-infection (0 weeks) and at 1–6, 10, and 12 weeks after that. The serum levels of seven cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-13, TGF-β, and IL-17) were simultaneously determined using ELISA and further analyzed.ResultsIn the present study, no significant changes in Th1-type cytokines production were detected in early infection, both in primary and secondary infections, while the Th2-type response was strongly induced. A comparison of primary and secondary infection showed no significant difference in the cytokine secretion, which may indicate that the re-infection at 4 weeks after primary infection could not induce a robust adaptive immune response. The full extent of interaction between buffalo and F. gigantica in re-infection requires further study.

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Section: Introductionmentioning

confidence: 99%

Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

et al. 2023

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“…Buffalo are economically important animals in Asian and African countries, producing meat and milk but also serving as working animals in rural areas. In 2021, a survey, in which dissection was used for examining F. gigantica adult, suggested that all the flukes were F. gigantica, and no Fasciola hepatica or the intermediate form was found in Nanning, South of the China (5). Thus, F. gigantica infection in buffalo cannot be ignored.…”

Section: Introductionmentioning

confidence: 99%

Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo

Wang

He²,

Wu³

et al. 2022

Front. Vet. Sci.

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Effect of primary and secondary Fasciola gigantica infection on specific IgG responses, hepatic enzyme levels and weight gain in buffaloes

Wang

Zhendi

et al. 2022

Parasitol Res

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Identification of Fasciola spp. based on ITS-2 reveals the Fasciola gigantica infection in buffaloes in Nanning city, South China

Cited by 4 publications

References 22 publications

Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo

Effect of primary and secondary Fasciola gigantica infection on specific IgG responses, hepatic enzyme levels and weight gain in buffaloes

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