Identification of Floral Scent in Chrysanthemum Cultivars and Wild Relatives by Gas Chromatography-Mass Spectrometry

Abstract

The objective of this study was to identify the major volatile compounds and their relative concentrations in flowers of different chrysanthemum cultivars and their wild relatives. The volatile organic components of fresh flowers were analyzed using a headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry. In total, 193 volatile organic components were detected; the major scent components were monoterpenoids and oxygenated monoterpenoids, which accounted for 68.59%–99.93% of the total volatiles in all tested materials except for Chrysanthemum indicum collected from Huangshan, in which they accounted for only 37.45% of total volatiles. The major volatile compounds were camphor, α-pinene, chrysanthenone, safranal, myrcene, eucalyptol, 2,4,5,6,7,7ab-hexahydro-1H-indene, verbenone, β-phellandrene and camphene. In a hierarchical cluster analysis, 39 accessions of Chrysanthemum and its relatives formed six clusters based on their floral volatile compounds. In a principal component analysis, only spider type flowers were located closely on the score plot. The results of this study provide a basis for breeding chrysanthemum cultivars which desirable floral scents.

The Chrysanthemum nankingense Genome Provides Insights into the Evolution and Diversification of Chrysanthemum Flowers and Medicinal Traits

“…Chrysanthemums are also important as health foods and as anti-inflammatory herbs in traditional Chinese medicine. Recent pharmacological studies have focused on their various biological effects including antioxidative, cardiovascular protective, vasorelaxant, and antihuman immune deficiency virus activities (Kim and Lee, 2005;Woo et al, 2008;Wang et al, 2014;Sun et al, 2015). However, despite their economic importance and beneficial properties, chrysanthemum evolution and the underlying molecular basis of the diversity of chrysanthemums are poorly understood.…”

Geographical origin identification and quality control of Chinese chrysanthemum flower teas using gas chromatography–mass spectrometry and olfactometry and electronic nose combined with principal component analysis

“…The content of volatile components was calculated according to Sun et al . (). where C vc is the content of volatile components, C is is the content of internal standard, A vc is the peak area of volatile components, and A is is the peak area of internal standard. Additionally, the blended internal standard in this work consists of n‐heptanol and methyl undecanoate.…”

“…The RI values were calculated according to the method of Song et al (2013). The content of volatile components was calculated according to Sun et al (2015).…”

ANÁLISE DA COMPOSIÇÃO QUÍMICA E DE ACEITAÇÃO SENSORIAL DA FRAGRÂNCIA DA FLOR MASCULINA DO BURITIZEIRO (Mauritia flexuosa L.f.; Arecaceae)

“…Da Palmeira-B efetuou-se a captação dos compostos voláteis tanto de uma (01) ráquila isolada (B), como do conjunto delas (B'); da Palmeira-C foi utilizada toda a inflorescência, destacando-se que houve um intervalo de 24 horas entre a sua coleta e a efetiva captação dos voláteis, portanto o material se encontrava em estágio de degeneração. As amostras foram previamente acondicionadas em invólucro de poliacetato por um período mínimo de 20 minutos (SUN et al, 2015) e, então, submetidas à captação dos compostos voláteis pela técnica do headspace (JÜRGENS et al, 2006) Compostos voláteis foram analisados através de cromatografia gasosaespectrometria de massa (CG-EM) em espectrômetro de massa Thermo Finningan Voyager™ acoplado a um cromatógrafo Thermo Trace GC 2000™ (Thermo Fisher Scientific Inc., Rockford, USA), equipado com uma coluna CP-Wax 52CB (Varian, Inc.; 30 m x 0,25 mm d.i., 0,25 µm espessura da película). Para cada amostra, 0,6 µl de extrato foi injetado diretamente na coluna.…”