Identification of G protein‐coupled receptors required for vitellogenesis and egg development in an insect with panoistic ovary

Zheng, H.; Zeng, Baojuan; Shang, Tiantian; Zhou, Shutang

doi:10.1111/1744-7917.12841

Cited by 16 publications

(21 citation statements)

References 56 publications

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“…LmDDX3 knockdown led to small underdeveloped terminal oocytes (Figure 1d). Similar phenotype was also reported in other genes' knockdown lines (Guo et al, 2019; Jing et al, 2018; Luo et al, 2017; Song et al, 2013; Song et al, 2014; Song et al, 2018; Tetlak et al, 2015; Tokar et al,2014; Wang et al, 2017; Wu et al, 2016; Wu et al, 2018; Zheng et al, 2020), and all these genes or factors were indicated to be involved in vitellogenesis, a key process of oocyte development and maturation in oviparous animals including birds, fish, frog, mammalian, and insects (Polzonetti‐Magni et al, 2004; Ruan et al, 2020; Valle, 1993).…”

Section: Discussionsupporting

confidence: 81%

“…(f) Grp78‐1 expression in the fat bodies of female adults. (g) A modified model from Zheng et al (2020) for LmDDX3 function in locust ovarian development and oocyte maturation. LmDDX3 directly or indirectly promotes LmVg , at least partially via JH signaling pathway, and regulates LmVgR for oocyte maturation and ovarian growth.…”

Section: Resultsmentioning

confidence: 99%

“…Although the female gonad is formed at early stages of nymph, the oocyte maturation and oviposition occur only in adults. Many factors have been revealed to be directly or indirectly involved in the process, such as juvenile hormone (JH) and cell cycle regulators (Wu et al, 2018), zinc finger transcription factor (Song et al, 2014), Na+/K+‐ATPase (Jing et al, 2018), G protein‐coupled receptors (Zheng et al, 2020) and microRNAs (miRNAs; Song & Zhou, 2020; Song et al, 2019), etc. These factors may become putative targets for biological control of the locust, which is good for ecological environment protection.…”

Section: Introductionmentioning

confidence: 99%

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The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Wang

Deng

et al. 2021

Arch Insect Biochem Physiol

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DDX3 represents a well‐defined subfamily of DEAD‐box RNA helicase and exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility. Our previous study has shown that LmDDX3, the ortholog of DDX3 in Locusta migratoria, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown of LmDDX3 results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. In this study, we therefore characterized LmDDX3 expression in female adult locust and analyzed its function in oocyte development. LmDDX3 was expressed in all tissues examined with significant more transcripts in ovary and hindgut. In ovary, a strong expression level was detected at the day just after adult eclosion, and a dramatic reduction then occurred during the oocyte development. LmDDX3 RNAi led to a reduced vitellogenin (Vg) expression in fat body via partially at least, the JH signaling pathway, and caused an upregulation of vitellogenin receptor (VgR) in ovary, and thus blocked the ovarian development and oocyte maturation. Sequence and phylogenetic analysis indicated that LmDDX3 was closely related to termite DDX3. Taken together, these data reveal a critical role for LmDDX3 in regulating the transcription of Vg and VgR, two major factors in vitellogenesis that is a key process required for ovary development and oocyte maturation in locust, and contribute thereof a new putative target for locust biological control.

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Section: Discussionsupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Wang

Deng

et al. 2021

Arch Insect Biochem Physiol

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“…In L. migratoria , 22 GPCRs were identified in the ovarian transcriptome of vitellogenic females. RNAi screening revealed that Orphan receptor-A (OR-A1 and OR-A2), C-type muscarinic acetylcholine receptor (mAChR-C) and Cirl-like (CirlL) played key roles in ovarian Vg transportation and uptake (Zheng et al, 2020 ).…”

Section: Regulation Of Vg Uptake By Ovariesmentioning

confidence: 99%

Regulatory Mechanisms of Vitellogenesis in Insects

Yang

et al. 2021

Front. Cell Dev. Biol.

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126

109

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Vitellogenesis is pre-requisite to insect egg production and embryonic development after oviposition. During insect vitellogenesis, the yolk protein precursor vitellogenin (Vg) is mainly synthesized in the fat body, transported by the hemolymph through the intercellular spaces (known as patency) in the follicular epithelium to reach the membrane of maturing oocytes, and sequestered into the maturing oocytes via receptor-mediated endocytosis. Insect vitellogenesis is governed by two critical hormones, the sesquiterpenoid juvenile hormone (JH) and the ecdysteriod 20-hydroxyecdysone (20E). JH acts as the principal gonadotropic hormone to stimulate vitellogenesis in basal hemimetabolous and most holometabolous insects. 20E is critical for vitellogenesis in some hymenopterans, lepidopterans and dipterans. Furthermore, microRNA (miRNA) and nutritional (amino acid/Target of Rapamycin and insulin) pathways interplay with JH and 20E signaling cascades to control insect vitellogenesis. Revealing the regulatory mechanisms underlying insect vitellogenesis is critical for understanding insect reproduction and helpful for developing new strategies of insect pest control. Here, we outline the recent research progress in the molecular action of gonadotropic JH and 20E along with the role of miRNA and nutritional sensor in regulating insect vitellogenesis. We highlight the advancements in the regulatory mechanisms of insect vitellogenesis by the coordination of hormone, miRNA and nutritional signaling pathways.

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“…Gene specific primers (GSPs) were combined with GSPs containing the T7 RNA polymerase promoter sequence ( Table 1 ). As an experimental control, a dsRNA based on the Ampicillin Resistance Gene ( dsARG) from the pGEM-T Easy Vector system (Promega, WI, USA) was used throughout the study ( 38 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of Gonadulin and Insulin-Like Growth Factor From Migratory Locusts and Their Importance in Reproduction in Locusta migratoria

et al. 2021

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Many insect species have several genes coding for insulin-related peptides (IRPs), but so far only a single IRP gene has been identified in migratory locusts. Here, we report and characterize two other genes coding for peptides that are related to insulin, namely gonadulin and arthropod insulin-like growth factor (aIGF); peptides postulated to be orthologs of Drosophila melanogaster insulin-like peptides 8 and 6 respectively. In Locusta migratoria the aIGF transcript is expressed in multiple tissues as was previously reported for IRP in both L. migratoria and Schistocerca gregaria, but there are significant differences in expression patterns between the two species. The gonadulin transcript, however, seems specific to the ovary, whereas its putative receptor transcript is expressed most abundantly in the ovary, fat body and the central nervous system. Since the central nervous system-fat body-ovary axis is essential for successful reproduction, we studied the influence of gonadulin on vitellogenesis and oocyte growth. A reduction in the gonadulin transcript (via RNA interference) led to a significant reduction in vitellogenin mRNA levels in the fat body and a strong oocyte growth inhibition, thus suggesting an important role for gonadulin in reproduction in this species.

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Identification of G protein‐coupled receptors required for vitellogenesis and egg development in an insect with panoistic ovary

Cited by 16 publications

References 56 publications

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Regulatory Mechanisms of Vitellogenesis in Insects

Identification of Gonadulin and Insulin-Like Growth Factor From Migratory Locusts and Their Importance in Reproduction in Locusta migratoria

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