Identification of G proteins mediating fungal elicitor-induced dephosphorylation of host plasma membrane H<sup>+</sup>-ATPase

Xing, T.; Higgins, Verna J.; Blumwald, Eduardo

doi:10.1093/jxb/48.2.229

Cited by 34 publications

(32 citation statements)

References 43 publications

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“…The increase in the host-plasma membrane H fATPase activity, changes in the plasma membrane redox activities (Vera-Estrella et al, 1994a, 1994b, the induction of a protein kinase cascade mediating the phospholdephosphorylation of the plasma membrane H+-ATPase (Xing et al, 1996), and the activation of a stimulatory type of heterotrimeric G-proteins (Xing et al, 1997) have also been shown to be specifically induced on Cf5 tomato cells by IF,.…”

Section: Discussionmentioning

confidence: 99%

“…Previous work with tomato Cf5 cell-suspension cultures showed that treatment with IFs containing the avr5 elicitor (IF,) led to a heterotrimeric G-protein-mediated dephosphorylation of the host-plasma membrane H+-ATPase. This resulted in the stimulation of the H'-pump activity with the concomitant hyperpolarization of the electrical potential difference across the plasma membrane and the acidification of the extracellular milieu (Vera-Estrella et al, 1994a;Xing et al, 1997).…”

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confidence: 99%

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Activation of Plant Plasma Membrane Ca2+-Permeable Channels by Race-Specific Fungal Elicitors

1997

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The response of plant cells t o invading pathogens is regulated by fluctuations in cytosolic Ca2+ levels that are mediated by Ca2+-permeable channels located at the plasma membrane of the host cell. The mechanisms by which fungal elicitors can induce Ca2+ uptake by the host cell were examined by the application of conventional patch-clamp techniques. Whole-cell and single-channel experiments on tomato (Lycopersicon esculentum L.) protoplasts revealed a race-specific fungal elicitor-induced activation of a plasma membrane Caz+-permeable channel. The presence of the fungal elicitor resulted in a greater probability of channel opening. Cuanosine 5'-[P-thioldiphosphate, a CDP analog that locks heterotrimeric C-proteins into their inactivated state, abolished the channel activation induced by the fungal elicitor, whereas guanosine 5'[y-thioltriphosphate, a nonhydrolyzable CTP analog that locks heterotrimeric C-proteins into their activated state, produced an effect similar t o that observed with the fungal elicitor. Mastoparan, which stimulates CTPase activity, mimicked the effect of CTP[ylS. The addition of HA1004 (a protein kinase inhibitor) in the presence of the elicitor totally abolished channel activity, whereas okadaic acid (a protein phosphatase inhibitor) moderately enhanced channel activity, suggesting that the activation of the channel by fungal elicitors is modulated by a heterotrimeric C-protein-dependent phosphorylation of the channel protein.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Activation of Plant Plasma Membrane Ca2+-Permeable Channels by Race-Specific Fungal Elicitors

1997

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“…For example, it has been demonstrated that elicitor-induced stimulation of PLC is mediated by G-proteins (Legendre et al 1993). Other studies have reported evidence for activation of G-proteins, thus showing that elicitor treatment of a multimeric complex dissociates a 42 kDa protein that cross-reacts with Ga antibodies (Xing et al 1997).…”

Section: Introductionmentioning

confidence: 94%

Involvement of G‐proteins in chitosan‐induced Anthraquinone synthesis in Rubia tinctorum

Vasconsuelo

Picotto

Giuletti

et al. 2006

Physiologia Plantarum

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We have previously shown that chitosan stimulates anthraquinone synthesis in Rubia tinctorum L. cells through activation of the PLC/PKC, PI3K, MAPK and Ca 2þ messenger systems. In view of this evidence, we have now investigated whether guanine nucleotide-binding G-proteins are part of the signal transduction mechanism which mediates the elicitor action. The G-protein agonists mastoparan, AlF 4 -and GTPyS increased anthraquinone levels to the same extent as chitosan. No additive effects were observed when cultured R. tinctorum cells were treated with agonist and the elicitor together. In agreement with these observations, the G-protein antagonists suramin and GDPbS abolished the increase in anthraquinone synthesis induced by chitosan. Furthermore, elicitation was not affected in the presence of pertussis toxin. Consistent with this result, when cell cultures were preincubated with a monoclonal anti-Gaq/11 antibody, the chitosan-dependent increase in anthraquinone levels was fully inhibited. Moreover, the presence of an immunoreactive protein of the expected size for Gaq/11 (42 kDa) was observed in R. tinctorum microsomal membranes by Western blot analysis using the same antibody. These results indicate that chitosan stimulates anthraquinone synthesis in R. tinctorum cells through a heterotrimeric G-protein, most likely belonging to the Gaq family.

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“…Mathieu et al [46] have characterized an increase in cytoplasmic H + as a common early response of tobacco cells to elicitors and also showed that the uptake of H + by tobacco cells was mediated by protein phosphorylation. Proton-ATPases and G proteins may also be involved in the elicitor-induced import of H + [47], and the H + import observed with the 24-kDa protein is likely linked to the K + efflux (Fig. 8).…”

Section: Cell Culture Experimentsmentioning

confidence: 99%

Induction of defense responses in tobacco by the protein Nep1 from Fusarium oxysporum

et al. 2001

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Identification of G proteins mediating fungal elicitor-induced dephosphorylation of host plasma membrane H⁺-ATPase

Cited by 34 publications

References 43 publications

Activation of Plant Plasma Membrane Ca2+-Permeable Channels by Race-Specific Fungal Elicitors

Activation of Plant Plasma Membrane Ca2+-Permeable Channels by Race-Specific Fungal Elicitors

Involvement of G‐proteins in chitosan‐induced Anthraquinone synthesis in Rubia tinctorum

Induction of defense responses in tobacco by the protein Nep1 from Fusarium oxysporum

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Identification of G proteins mediating fungal elicitor-induced dephosphorylation of host plasma membrane H+-ATPase

Cited by 34 publications

References 43 publications

Activation of Plant Plasma Membrane Ca2+-Permeable Channels by Race-Specific Fungal Elicitors

Activation of Plant Plasma Membrane Ca2+-Permeable Channels by Race-Specific Fungal Elicitors

Involvement of G‐proteins in chitosan‐induced Anthraquinone synthesis in Rubia tinctorum

Induction of defense responses in tobacco by the protein Nep1 from Fusarium oxysporum

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Identification of G proteins mediating fungal elicitor-induced dephosphorylation of host plasma membrane H⁺-ATPase