Identification of genes expressed in response to yellow head virus infection in the black tiger shrimp, Penaeus monodon, by suppression subtractive hybridization

Prapavorarat, Adisak; Pongsomboon, Siriporn; Tassanakajon, Anchalee

doi:10.1016/j.dci.2010.01.002

Cited by 53 publications

(32 citation statements)

References 59 publications

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“…In fact, 10 out of 15 species studied showed antibacterial activity. These types of proteins usually are not identified in mollusks [21], but it could have an important role in defense, as in the case of crustaceans [23]. Even in mammals that show a developed adaptive immune system, the antimicrobial proteins play a prominent role in defense [1,2,9].…”

Section: Resultsmentioning

confidence: 98%

Screening of Antimicrobials from Caribbean Sea Animals and Isolation of Bactericidal Proteins from the Littoral Mollusk Cenchritis muricatus

et al. 2012

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Section: Resultsmentioning

confidence: 98%

Screening of Antimicrobials from Caribbean Sea Animals and Isolation of Bactericidal Proteins from the Littoral Mollusk Cenchritis muricatus

et al. 2012

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“…In these studies WSSV genomes could not be profiled in the SHH libraries but differentially expressed genes were. However, in the other studies on penaeids experimentally infected with WSSV (García et al, 2009;Reyes et al, 2007) and YHV (Junkunlo et al, 2010;Prapavorarat et al, 2010) both host genes and viral genes were identified in the SSH libraries. These discrepancies may indicate the variability of the suppression subtractive hybridization method in profiling viral genomes in given samples.…”

Section: Discussionmentioning

confidence: 99%

Investigation of an Idiopathic Lesion in Redclaw Crayfish Cherax Quadricarinatus Using Suppression Subtractive Hybridization

Rusaini¹,

Ariel²,

Burgess³

et al. 2013

JVM

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Lesions indicative of putative parvovirus infection were detected in a population of freshwater redclaw crayfish (Cherax quadricarinatus) displaying hypertrophied nuclei, while these changes were undetected in another population. These changes were characterised by hypertrophic nuclei with marginated chromatin but without Cowdry type A (CA) intranuclear inclusion bodies. Suppression subtractive hybridization (SSH) was performed to generate a cDNA forward library between the two populations of crayfish. A total of 323 sequences were analysed from SSH libraries. These sequences were grouped into 76 consensus sequences and clustered into 8 categories based on sequence homology from the NCBI GenBank database. Even though histopathological observations consistently revealed lesions presumptive of putative parvovirus in C. quadricarinatus as previously reported, the molecular method failed to confirm a viral aetiology. Despite the absence of viral gene detection and therefore a conclusion of an idiopathic aetiology, the health status of these two populations of redclaw crayfish was different, resulting in differentially expressed immune-related genes in the two populations, with some genes being up-regulated in the hypertrophied nuclei population. Furthermore, the absence of parvovirus from the SSH libraries indicates that perhaps the viral messenger RNA was in too low a concentration to be detected or does not have any poly(A) tail which the SSH methodology needs to function correctly.

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“…Preechapho et al, isolated 109 known sequences and identified several reproduction-related transcripts in ovaries of the giant tiger shrimps (P. monodon) using SSH (Preechaphol, Klinbunga, Khamnamtongand, & Menasveta, 2010). Adisak et al, obtained 185 transcripts and uncovered 8 novel YHV-responsive genes from haemocyte libraries of P. monodon induced by yellow head virus after 24h and 48/72h (Prapavorarat, Pongsomboon, & Tassanakajon, 2010). Four suppression subtractive hybridization (SSH) cDNA libraries were constructed to identify differentially expressed salinity stress responsive genes of black tiger shrimp, Penaeus monodon exposed to low (3 ppt) salinity and high (50 ppt) salinity conditions (Shekhar et al, 2013;Shekhar et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Liu¹,

Sun²,

Dong³

et al. 2016

IJB

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Suppression subtractive hybridization (SSH) was applied to screen responsively up-regulation genes in hepatopancreas and gill of Litopenaeus vannamei induced by acute hypo-osmotic stress. In the hepatopancreas, 196 clones were randomly selected and sequenced. 131 non-redundant transcripts, corresponding to 41 genes, were found with elevated expressions. They were functionally clustered into eight biological processes which were protein synthesis and processing, carbohydrate metabolism and energy production, transport, cell growth, apoptosis, cytoskeletal component, cell defense and homeostasis, signal transduction, accounting for 26.2%, 14.3%, 16.7%, 4.7%, 4.7%, 2.3%, 11.9% and 7.1%, respectively. When confirmed by real time qRT-PCR, the gene expression levels of MnSOD, glutathione and glutamine synthetase showed significant increases (2.64-folds, 3.44-folds and 2.16-folds, respectively) in hepatopancreas (P<0.05). Totally 81 expressed sequence tags (ESTs) were obtained by random sequencing from the gill SSH cDNA library, and 52 unigenes, including 15 contigs and 37 singlets, after CP3 online matching were gained by clustering and assembling. The sequence alignment results revealed that 30 of them had significant homology to previously identified Genbank database sequences, while 22 unigenes did not match any sequence in the datebase and presumably represented unidentified cDNAs. Functionally, the unigenes were classified into six clusters, including ion channels and transport proteins (1); protein synthesis, translation and transcription factor (2); stress resistance and antioxidant factors (3); energy metabolism (4); signal receptor and transduction (5); cell fibrin and cytoskeletal proteins (6) with proportion of 23.3%, 20.0%, 20.0%, 16.7%, 10.0% and 10.0% respectively. Real time qRT-PCR confirmation revealed significant elevation of Arginine kinase, Carbonic anhydrase and NaK-ATPase-α-subunit expressions (3.73-folds, 2.55-folds and 5.83-folds, respectively) in the gill after acute low-osmotic stress (P < 0.05). These results provided insight into critical physiology processes and pathways constituting the hypo-osmotic stress adaption program in hepatopancreas and gill of L. vannamei.

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Identification of genes expressed in response to yellow head virus infection in the black tiger shrimp, Penaeus monodon, by suppression subtractive hybridization

Cited by 53 publications

References 59 publications

Screening of Antimicrobials from Caribbean Sea Animals and Isolation of Bactericidal Proteins from the Littoral Mollusk Cenchritis muricatus

Screening of Antimicrobials from Caribbean Sea Animals and Isolation of Bactericidal Proteins from the Littoral Mollusk Cenchritis muricatus

Investigation of an Idiopathic Lesion in Redclaw Crayfish Cherax Quadricarinatus Using Suppression Subtractive Hybridization

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

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