Identification of group B rotavirus as an etiological agent in the gastroenteritis outbreak in Maharashtra, India

Joshi, M. V.; Ganorkar, Nital N.; Ranshing, Sujata S.; Basu, Atanu; Chavan, Nutan; Gopalkrishna, Varanasi

doi:10.1002/jmv.24901

Cited by 11 publications

(18 citation statements)

References 21 publications

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“…A unique amino acid substitution at the VP7‐76 position (Lysine→Arginine) was noted similar to Chinese lineage strains (ADRV/1982, WH‐1/2002). This observation is in concordance with earlier outbreak reported from Pargaon, Western India in 2015 …”

Section: Discussionsupporting

confidence: 93%

“…The restriction of RVB outbreaks mainly to adolescent and adult age group individuals was observed in the present study similar to earlier reports from India and China . Lahon et al suggested that the low infection rates observed in children may be due to either low exposure of children or fecal shedding of the virus below the detection limit of the assay employed in the study.…”

Section: Discussionsupporting

confidence: 91%

“…Till date, occurrence of RVB in five different gastroenteritis outbreaks has been documented from India, namely Daman (October 2000, Union territory of India), Surat (February 2004, Gujarat state), Mumbai (March 2006 and October 2006, Maharashtra state), and Sangli (May 2009, Maharashtra state) . The role of RVB as an etiological agent was reported for Pargaon outbreak (November 2015, Maharashtra state) and in Devli Karad outbreak of the present study (November 2017, Maharashtra state). It should be noted that a total of four outbreaks out of seven were reported between October and December and two outbreaks with confirmed RVB etiology were in the month of November indicating winter seasonal pattern.…”

Section: Discussionmentioning

confidence: 99%

“…The circulation of RVC, in individuals of all age groups has been documented in sporadic and large outbreak cases from different parts of the world . In contrast, RVB infections in sporadic and outbreak cases are mainly restricted to Asian countries namely China, India, Bangladesh, Myanmar, and Nepal and largely during gastroenteritis outbreaks in adults . Among non‐rota viral agents, Norovirus , Astrovirus, and Adenovirus infections have been reported to be predominant and known to be responsible for different gastroenteritis outbreaks globally .…”

Section: Introductionmentioning

confidence: 99%

“…4 In contrast, RVB infections in sporadic and outbreak cases are mainly restricted to Asian countries namely China, India, Bangladesh, Myanmar, and Nepal and largely during gastroenteritis outbreaks in adults. [5][6][7][8][9][10][11][12][13] Among non-rota viral agents, Norovirus, Astrovirus, and Adenovirus infections have been reported to be predominant and known to be responsible for different gastroenteritis outbreaks globally. [14][15][16] The transmission of these viruses could be water-borne, food-borne, person-to-person, and a variety of less clearly identifiable modes.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of a large waterborne acute gastroenteritis outbreak caused by group B rotavirus in Maharashtra state, India

Joshi

Lole

Barve³

et al. 2019

Journal of Medical Virology

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An acute gastroenteritis outbreak at Devli Karad village, Maharashtra, India with an attack rate of 22.6% affected mainly adolescent and adult population. The viral investigations conducted on fecal specimens of patients hospitalized indicated the presence of rotavirus B (RVB) using RNA polyacrylamide gel electrophoresis and reverse transcription polymerase chain reaction. The samples collected from the source of drinking water also showed the presence of the only RVB. Absence of other viral agents and identification of RVB of genotype G2 as the etiological agent of the acute gastroenteritis outbreak highlights, the necessity of monitoring RVB, the viral agent known for its large outbreak potential.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Investigation of a large waterborne acute gastroenteritis outbreak caused by group B rotavirus in Maharashtra state, India

Joshi

Lole

Barve³

et al. 2019

Journal of Medical Virology

Self Cite

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Recent advances in rotavirus reverse genetics and its utilization in basic research and vaccine development

Uprety

Wang

2021

Arch Virol

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Rotaviruses are segmented double-stranded RNA viruses with a high frequency of gene reassortment, and they are a leading cause of global diarrheal deaths in children less than 5 years old. Two-thirds of rotavirus-associated deaths occur in low-income countries. Currently, the available vaccines in developing countries have lower efficacy in children than those in developed countries. Due to added safety concerns and the high cost of current vaccines, there is a need to develop cost-effective next-generation vaccines with improved safety and efficacy. The reverse genetics system (RGS) is a powerful tool for investigating viral protein functions and developing novel vaccines. Recently, an entirely plasmid-based RGS has been developed for several rotaviruses, and this technological advancement has significantly facilitated novel rotavirus research. Here, we review the recently developed RGS platform and discuss its application in studying infection biology, gene reassortment, and development of vaccines against rotavirus disease. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-021-05142-7.

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Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity

Veletanlic¹,

Sartalamacchia²,

Diller³

et al. 2023

Preprint

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Fusion-associated small transmembrane (FAST) proteins are viral nonstructural proteins that mediate cell-cell fusion to form multinucleated syncytia. We previously reported that human species B rotavirus NSP1-1 is a FAST protein that induces syncytia in primate epithelial cells but not rodent fibroblasts. We hypothesized that the NSP1-1 proteins of other rotavirus species could also mediate cell-cell fusion and that fusion activity might be limited to cell types derived from homologous hosts. To test this hypothesis, we predicted the structure and domain organization of NSP1-1 proteins of species B rotavirus from a human, goat, and pig, species G rotavirus from a pigeon and turkey, and species I rotavirus from a dog and cat. We cloned these sequences into plasmids and transiently expressed the NSP1-1 proteins in avian, canine, hamster, human, porcine, and simian cells. Regardless of host origin of the virus, each NSP1-1 protein induced syncytia in primate cells, while few induced syncytia in other cell types. To identify the domains that determined cell-specific fusion activity for human species B rotavirus NSP1-1, we engineered chimeric proteins containing domain exchanges with the p10 FAST protein from Nelson Bay orthoreovirus. Using the chimeric proteins, we found that the N-terminal and transmembrane domains determined the cell type specificity of fusion activity. Although the species and cell type criteria for fusion activity remain unclear, these findings suggest that rotavirus species B, G, and I NSP1-1 are functional FAST proteins whose N termini play a role in specifying the cells in which they mediate syncytia formation.

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Identification of group B rotavirus as an etiological agent in the gastroenteritis outbreak in Maharashtra, India

Cited by 11 publications

References 21 publications

Investigation of a large waterborne acute gastroenteritis outbreak caused by group B rotavirus in Maharashtra state, India

Investigation of a large waterborne acute gastroenteritis outbreak caused by group B rotavirus in Maharashtra state, India

Recent advances in rotavirus reverse genetics and its utilization in basic research and vaccine development

Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity

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