Identification of Group-I Introns at Three Different Positions within the 28S rDNA Gene of the Entomopathogenic Fungus Metarhizium anisopliae var. anisopliae

Mavridou, Annoula; Cannone, Jamie J.; Typas, Milton A.

doi:10.1006/fgbi.2000.1232

Cited by 22 publications

(18 citation statements)

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“…The results of analyses of group I introns suggest ancestry involving both horizontal and vertical acquisition for these elements in the GFC [53-57]. The large diversity of introns in the mt genomes of F. circinatum , F. verticillioides and F. fujikuroi and the other Fusarium species outside of this complex [22] indicates that the acquisition of these elements occurred multiple times and independently [53,55,56].…”

Section: Discussionmentioning

confidence: 99%

Evidence for inter-specific recombination among the mitochondrial genomes of Fusarium species in the Gibberella fujikuroi complex

et al. 2013

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BackgroundThe availability of mitochondrial genomes has allowed for the resolution of numerous questions regarding the evolutionary history of fungi and other eukaryotes. In the Gibberella fujikuroi species complex, the exact relationships among the so-called “African”, “Asian” and “American” Clades remain largely unresolved, irrespective of the markers employed. In this study, we considered the feasibility of using mitochondrial genes to infer the phylogenetic relationships among Fusarium species in this complex. The mitochondrial genomes of representatives of the three Clades (Fusarium circinatum, F. verticillioides and F. fujikuroi) were characterized and we determined whether or not the mitochondrial genomes of these fungi have value in resolving the higher level evolutionary relationships in the complex.ResultsOverall, the mitochondrial genomes of the three species displayed a high degree of synteny, with all the genes (protein coding genes, unique ORFs, ribosomal RNA and tRNA genes) in identical order and orientation, as well as introns that share similar positions within genes. The intergenic regions and introns generally contributed significantly to the size differences and diversity observed among these genomes. Phylogenetic analysis of the concatenated protein-coding dataset separated members of the Gibberella fujikuroi complex from other Fusarium species and suggested that F. fujikuroi (“Asian” Clade) is basal in the complex. However, individual mitochondrial gene trees were largely incongruent with one another and with the concatenated gene tree, because six distinct phylogenetic trees were recovered from the various single gene datasets.ConclusionThe mitochondrial genomes of Fusarium species in the Gibberella fujikuroi complex are remarkably similar to those of the previously characterized Fusarium species and Sordariomycetes. Despite apparently representing a single replicative unit, all of the genes encoded on the mitochondrial genomes of these fungi do not share the same evolutionary history. This incongruence could be due to biased selection on some genes or recombination among mitochondrial genomes. The results thus suggest that the use of individual mitochondrial genes for phylogenetic inference could mask the true relationships between species in this complex.

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Section: Discussionmentioning

confidence: 99%

Evidence for inter-specific recombination among the mitochondrial genomes of Fusarium species in the Gibberella fujikuroi complex

et al. 2013

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“…In entomopathogenic species, there is substantial variation both in the site of insertion and in the primary structure of group I introns [7]. Characteristics of site insertion and intron type can be used to provide both phylogenetic information and unique profiles for individual isolates within these species [8,15,27].…”

Section: Resultsmentioning

confidence: 99%

“…In the fungi, LSU group I introns are present in several symbiotic and parasitic species, and have been identified in fungal pathogens of plants [5,6], insects [7,8] and humans [9,10]. There are usually four invariant recognition sites in the 3?…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi

et al. 2009

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Group I introns have the ability to catalyse their own excision (self-splice) from pre-RNA, and are found in a wide range of eukaryotic organisms. In fungal nuclear genomes, they have been identified in the small subunit (SSU) and large subunit (LSU) of the ribosomal RNA gene. Sequencing of the 3' region of the LSU rRNA gene of the dermatophyte Trichophyton interdigitale revealed a 393 bp group I intron, Tin.2563, containing the four characteristic conserved motifs (P,Q,R and S) essential for self-splicing. The predicted secondary structure revealed nine sets of conserved paired regions (P1-P9), with most similarity to a subgroup IE intron of the entomopathogenic hyphomycete Beauveria bassiana. Tin.2563 was inserted at a site in the LSU rDNA corresponding to position 2563 of the Escherichia coli 23S rRNA. PCR and sequence analysis showed an intron to be present at an identical location in the LSU rDNA of many dermatophytes, although its distribution was erratic. In contrast, an intron was present at the same location in multiple isolates (n = 20) of the clinically important anthrophilic species Trichophyton rubrum and T. interdigitale. Conservation of intron insertion site, subgroup and P helix sequences showed intron genotyping to be unsuitable for strain identification in dermatophytes. Phylogenetic analysis of intron sequences from different dermatophyte species indicated that lateral transfer of the element was likely to be a rare event.

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“…Estrada et al (2007) studied 11 isolates and found that seven isolates showed exclusive bands and that ISSR primer 873 was able to distinguish all the isolates. Studies with respect to introns of group I have sought to identify them and check for polymorphism among the isolates of M. anisopliae by way of phylogenetic analysis (Mavridou et al, 2000;Márquez et al, 2006). This technique was also successfully applied in polymorphism studies of B. bassiana (Wang et al, 2003) and B. brongniartii (Neuvéglise et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…The ISSR technique was used in studying the genetic variability of two varieties of M. anisopliae from different localities (Lima, 2005) and Beauveria bassiana (Balsamo) Vuillemin in China (Wang et al, 2005), Asia (Aquino de Muro et al, 2005) and Japan (Takatsuka, 2007). Group I introns, found in rDNA genes of eukaryotes, usually have irregular distribution, being present in some isolates and absent in others, which can become a source of genetic variability (Mavridou et al, 2000). Markers such as RAPD (random amplified polymorphic DNA), SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphism) have also been deployed in genetic variability studies of isolates of M. anisopliae from different hosts and geographic origins (Velásquez et al, 2007;Inglis et al, 2008;Freed et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers

Tiago¹,

Carneiro-Leão²,

Lima³

et al. 2011

Genet. Mol. Res.

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ABSTRACT.Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirtyseven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará da Serra, Southwest Mato Grosso State, Brazil. Internal transcribed spacer-RFLP did not differentiate the isolates of M. anisopliae var. anisopliae, while ISSR and intron primers identified three distinct groups. Variability among these groups was 96% for (GTG) 5 and 100% for the other primers. We found considerable genetic variability, even among isolates from the same geographical origin and host.

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Identification of Group-I Introns at Three Different Positions within the 28S rDNA Gene of the Entomopathogenic Fungus Metarhizium anisopliae var. anisopliae

Cited by 22 publications

References 54 publications

Evidence for inter-specific recombination among the mitochondrial genomes of Fusarium species in the Gibberella fujikuroi complex

Evidence for inter-specific recombination among the mitochondrial genomes of Fusarium species in the Gibberella fujikuroi complex

Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi

Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers

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