Identification of hydrophobin genes and their physiological functions related to growth and development in Pleurotus ostreatus

Xu, Danyun; Wang, Yuanyuan; Keerio, Aafaque Ahmed; Ma, Aimin

doi:10.1016/j.micres.2021.126723

Cited by 25 publications

(20 citation statements)

References 65 publications

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“…Hydrophobins (HFBs) are small proteins found only in filamentous fungi (Dikarya) [ 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. HFBs are characterized by their small size and amphipathic nature at hydrophilic/hydrophobic interfaces, and are currently attracting great interest from the biotechnology industry [ 38 , 39 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Novel Hydrophobin, Sa-HFB1, with Antifungal Activity from an Alkaliphilic Fungus, Sodiomyces alkalinus

Куварина¹,

Рогожин

Sykonnikov³

et al. 2022

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The adaptations that alkaliphilic microorganisms have developed due to their extreme habitats promote the production of active natural compounds with the potential to control microorganisms, causing infections associated with healthcare. The primary purpose of this study was to isolate and identify a hydrophobin, Sa-HFB1, from an alkaliphilic fungus, Sodiomyces alkalinus. A potential antifungal effect against pathogenic and opportunistic fungi strains was determined. The MICs of Sa-HFB1 against opportunistic and clinical fungi ranged from 1 to 8 µg/mL and confirmed its higher activity against both non- and clinical isolates. The highest level of antifungal activity (MIC 1 µg/mL) was demonstrated for the clinical isolate Cryptococcus neoformans 297 m. The hydrophobin Sa-HFB1 may be partly responsible for the reported antifungal activity of S. alkalinus, and may serve as a potential source of lead compounds, meaning that it can be developed as an antifungal drug candidate.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Novel Hydrophobin, Sa-HFB1, with Antifungal Activity from an Alkaliphilic Fungus, Sodiomyces alkalinus

Куварина¹,

Рогожин

Sykonnikov³

et al. 2022

JoF

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“…However, direct evidence was only found in S. commune [ 56 ]. Recently, the role in the formation of primordia was verified in F. filiformis and P. ostreatus by RNAi [ 16 , 17 ]. In this study, it was confirmed that Cmhyd1 was necessary for primordium formation in C. militaris by gene deletion, complementation, overexpression and exogenous addition of CmHYD1.…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that a hydrophobin gene, Hyd9 , played an important role in the formation of aerial mycelia and primordia in Flammulina filiformis (Agaricales) by RNAi and overexpression [ 16 ]. Hydrophobin FBH1 in Pleurotus ostreatus (Agaricales) affected the mycelial growth rate and primordium formation by RNAi transformants [ 17 ]. There have only been some studies on the gene transcript level of hydrophobin genes.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobin CmHYD1 Is Involved in Conidiation, Infection and Primordium Formation, and Regulated by GATA Transcription Factor CmAreA in Edible Fungus, Cordyceps militaris

Xiao

Wang

Liu

et al. 2021

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Hydrophobins are a family of small proteins exclusively secreted by fungi, and play a variety of roles in the life cycle. Cmhyd1, one of the hydrophobin class II members in Cordyceps militaris, has been shown to have a high transcript level during fruiting body development. Here, deletion of Cmhyd1 results in reduction in aerial mycelia, conidiation, hydrophobicity and infection ability, and complete inhibition of pigmentation and primordium differentiation. Cmhyd1 plays roles in conidiation and cuticle-bypassing infection by regulating the transcripts of frequency clock protein, Cmfrq, and velvet protein, Cmvosa, as well as primordium formation via the mitogen-activated protein kinase signaling pathway. Cmhyd1 also participates in stress response, including tolerance of mycelia to osmotic and oxidative stresses, and conidia to high or low temperatures. CmAreA, a transcription factor of nitrogen regulatory, is recruited to the promoter of Cmhyd1 and activates the transcription of Cmhyd1 with coactivator CmOTam using electrophoretic mobility shift assays and transient luciferase expression in tobacco. Furthermore, CmHYD1 is proved to regulate the transcription of Cmarea at different developmental stages via a positive feedback loop. These results reveal the diverse roles and regulation of Cmhyd1 in C. militaris, and provide insights into the developmental regulatory mechanism of mushrooms.

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“…Of the 40 hydrophobin genes that were differently expressed temporally and spatially in P. ostreatus , some were abundant in mono- and dikaryotic mycelia, others were expressed only in mono- or dikaryotic mycelia, and others again were expressed preferably in fruiting bodies. The substrate, natural light and temperature affected hydrophobin gene expression and the subsequent growth rate and primordia formation [ 18 ]. The influence of light on fruiting body formation has been supported by the identification of two blue light receptor genes in P. ostreatus [ 19 ].…”

Section: From Mycelium To Fruiting Body: Differential Gene Expressionmentioning

confidence: 99%

“…The few sources directly comparing protein concentration in fruiting body and mycelium found similar values [ 22 , 27 ]. These protein levels compete favourably with those of leguminous plants, such as pea, chickpea, soy or lupine [ 18 ]. In the fermentation processes, the mycelia are suspended in a liquid nutrient broth.…”

Section: From Genomics To Proteomics: Proteins Enzymes and Amino Acidsmentioning

confidence: 99%

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

et al. 2022

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Edible mushrooms are widely appreciated for their appealing flavours, low caloric values and high content of presumably health-protecting metabolites. Their long history of safe use together with the looming worldwide food crisis have revived the idea of generating meat analogues and protein isolates by the controlled fermentation of mycelia of these edible fungi as a dietary option. The occurrence of proteins, polysaccharides, smaller metabolites, metal ions and toxins in mycelia and fruiting bodies is compared among the three most popular species, Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), Lentinus edodes (shiitake) and some closely related species. Large effects of substrate chemistry, strain, developmental stage and ecological interactions result in a wide variation of the concentrations of some metabolites in both mycelial cells and fruiting bodies. This is obviously a result of the high adaptation abilities required to survive in natural habitats. Fungal bioprocesses are decoupled from agricultural production and can be operated anytime, anywhere, and on any scale according to demand. It is concluded that fungal biomass, if produced under food-grade conditions and on an industrial scale, could provide a safe and nutritious meat substitute and protein isolates with a high biological value for future vegan foods.

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Identification of hydrophobin genes and their physiological functions related to growth and development in Pleurotus ostreatus

Cited by 25 publications

References 65 publications

Isolation and Characterization of a Novel Hydrophobin, Sa-HFB1, with Antifungal Activity from an Alkaliphilic Fungus, Sodiomyces alkalinus

Isolation and Characterization of a Novel Hydrophobin, Sa-HFB1, with Antifungal Activity from an Alkaliphilic Fungus, Sodiomyces alkalinus

Hydrophobin CmHYD1 Is Involved in Conidiation, Infection and Primordium Formation, and Regulated by GATA Transcription Factor CmAreA in Edible Fungus, Cordyceps militaris

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

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