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A research was conducted to study the multiplication rates of banana shoot tips derived from different suckers under in vitro conditions during successive sub-culture of FHIA-23, KM5 and Basari and assessment of rapid amplified polymorphic DNA (RAPD) techniques of banana cultivars through molecular marker. Shoot tips of banana varieties were cultured on MS medium supplemented with different concentrations of Benzaline alkaline phosphate (BAP). MS ½ with Indole buric acid (IBA) 1 mgL-1 + 5.0 g, 10 g, 20 g, 30 g and 40 g sugar L-1 for root length, number of root and root weight were used for root induction. The results revealed that the best shoot initiation was observed in Basari using MS medium containing BAP 4 mg L-1. Maximum shoot length was observed in KM5 under the concentration of BAP 3.0 mg L-1. Maximum numbers of shoots and shoot weight were observed in KM5 followed by FHIA-23 and Basari variety. Maximum root length was observed in KM5 followed by FHIA-23 under the concentration of sugar 30 g L-1 + IBA 1.0 mg L-1. Maximum numbers of roots were observed in KM5, followed by FHIA-23 and Basari variety under concentration of 30 g L-1 + IBA 1.0 mg L-1. It was concluded that the KM5 variety was superior, followed by FHIA-23 and Basari. The concentration of 3.0 to 4.0 mg L-1 of BAP were found better for shooting and 1.0 mg L-1 of IBA + 30 and 40 g L-1 sugars for root induction of banana micropropagation.