Identification of Borrelia burgdorferi Ribosomal Protein L25 by the Phage Surface Display Method and Evaluation of the Protein's Value for Serodiagnosis

Abstract

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

BBK07 Immunodominant Peptides as Serodiagnostic Markers of Lyme Disease

“…The synthetic peptide C6, isolated from a conserved region of the variable membrane protein VlsE, is a target for host IgG and has been shown to be a sensitive and specific serodiagnostic marker (4,22). While purified antigens show great promise, no recombinant or synthetic antigen has demonstrated sufficient sensitivity to replace the current two-tier approach (7,14,16,20,24,26,27,35). Some of the highest sensitivities reported thus far have been obtained by the use of several antigens in combination to enhance diagnostic accuracy (4,8,19,31,32).…”

BBK07, a Dominant In Vivo Antigen ofBorrelia burgdorferi, Is a Potential Marker for Serodiagnosis of Lyme Disease

“…To reduce cross-reactivity, several recombinant B. burgdorferi antigens and various fragments thereof have been evaluated as serodiagnostic markers for LD, including OspC (35), BmpA (45), VlsE (27), BBK32 (22), L25 (33), P37 (31), and DbpA (20). OspC is exposed on the B. burgdorferi surface, is produced during early infection, and is highly immunogenic (1,13,16,35).…”