Identification of
            <i>Mycobacterium avium</i>
            subsp.
            <i>paratuberculosis</i>
            in Biopsy Specimens from Patients with Crohn's Disease Identified by In Situ Hybridization

Sechi, Leonardo Antonio; Manuela, Mura; Tanda, Francesco; Lissia, Amelia; Solinas, Antonio; Fadda, Giovanni; Zanetti, Stefania Anna Lucia

doi:10.1128/jcm.39.12.4514-4517.2001

Cited by 127 publications

(61 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell wall-defective mycobacteria were isolated also from skin lesions and cerebrospinal fluid of patients with sarcoidosis and identified to be M. a. paratuberculosis or other M. avium-intracellulare complex members (El-Zaatari et al, 1996). A relationship between cell wall deficient forms of M. a. paratuberculosis and Crohn's disease has been found by some authors, although this aetiological agent has not yet been conclusively proven (Hermon-Taylor and Bull, 2002;Hulten et al, 2001 a, b, c;Sechi et al ,2001;Schwartz et al, 2000).…”

Section: Clinical Significance and Role Of Mycobacterial L-formsmentioning

confidence: 99%

Cell Wall Deficiency in Mycobacteria: Latency and Persistence

Маркова¹

2012

Understanding Tuberculosis - Deciphering the Secret Life of the Bacilli

View full text Add to dashboard Cite

Section: Clinical Significance and Role Of Mycobacterial L-formsmentioning

confidence: 99%

Cell Wall Deficiency in Mycobacteria: Latency and Persistence

Маркова¹

2012

Understanding Tuberculosis - Deciphering the Secret Life of the Bacilli

View full text Add to dashboard Cite

“…Nearly one third of known Mycobacterium species have been observed to be associated with disease in humans [16]. The species of NTM associated with human disease are : M. avium, M. intracellulare [17,18], M. kansasii [19], M. paratuberculosis [20], M. scrofulaceum [21], M. simiae [22,23], M. interjectum [24], M. xenopi [25], M. szulgai [26], M. fortuitum [27], M. chelonae [28], M. marinum [29], M. genavense [30], M. ulcerans [31], M. smegmatis [32], M. thermoresistible [33], M. neoaurum [34], M. vaccae [35]. Presently little information is available on the evolution of RNR in different species of Mycobacterium which governs the central metabolic pathways by directing the DNA replication.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Mycobacterial Ribonucleotide Reductase (RNR) and its Implication as a Novel Drug Target

Mohanty¹,

Naaz²

2015

Bioenergetics

View full text Add to dashboard Cite

The genus Mycobacterium consists of both pathogenic and non-pathogenic species. The emergence of MDR and XDR tuberculosis and opportunistic infections by non tuberculous mycobacterium in immunosuppressive persons are major concern now a days. The aim of the study was to characterize ribonucleotide reductase (RNR) of Mycobacterium species to produce information about the evolution of the gene which could further be used in targeting RNR as a novel drug target. Here we were analyzed RNR of 23 mycobacterial species. The sequence length of RNR region is about 975 bp. Out of 975 characters 625 (64.10%) are conserved sites (monomorphic) and 350 (35.89%) are variable sites (polymorphic). The total nucleotide diversity (π) is=0.120114 (12.011%). The RNR phylogeny approach in Mycobacterium species provides evidence of several evolutionary lineages evolving from the ancestral polymorphism and fixed in the descendant population. Species of mycobacteria causing tuberculosis or respiratory infection in humans have specific patterns of allele distribution in different motifs, which differentiate them from other opportunistic mycobacterial species. Molecular analysis and structural motif analysis of RNR suggests the occurrence of host-mediated genetic differentiation in mycobacterial species, which requires further wet lab investigations.

show abstract

“…Por otra parte, se ha reportado la existencia de cepas de MAP con una pared celular reducida o inexistente, conocidas como formas L, que no se consideran ácido alcohol resistentes y por lo tanto, son indetectables mediante la tinción de Ziehl Neelsen, utilizada tradicionalmente para la observación de las bacterias del género Mycobacterium. Además, esta modifi cación de la pared celular de MAP disminuye su crecimiento en los medios de cultivo específi cos, pasando inadvertidas cuando se ha intentado identifi car en pacientes humanos 1,27,38,39 .…”

Section: La Asociación Map-ecunclassified

Mycobacterium avium subsp paratuberculosis y enfermedad de Crohn: evidencias de una zoonosis

et al. 2011

Rev. méd. Chile

View full text Add to dashboard Cite

L a paratuberculosis bovina, también conocida como enfermedad de Johne, es una patología causada por la bacteria Mycobacterium avium subsp. paratuberculosis (MAP), que se caracteriza por una lesión intestinal crónica de carácter proliferativa 1 . Debido a las similitudes fi siopatológicas entre la infección de los bovinos y la enfermedad de Crohn (EC) en el humano, una asociación causal del MAP con ambas patologías ha sido sugerida desde hace algunos años, lo que ha generado controversia en la comunidad científi ca relacionada a la transmisión inter-especies 1,2 . En este trabajo se revisan exhaustivamente los hallazgos más representativos y recientes que dan cuenta de esta controversia, y se describen brevemente los antecedentes en Chile que explican la necesidad de realizar estudios en este ámbito. El agenteMAP es un patógeno intracelular facultativo, Gram positivo, ácido alcohol resistente, aerobio y cuya temperatura óptima de crecimiento es 37ºC. Con un tiempo de generación mayor a 20 h, es una bacteria de crecimiento lento que requiere varias semanas de incubación antes que sus colonias sean detectables en los medios de cultivo 1,3,4 . Como las bacterias del género Mycobacterium, MAP posee un genoma rico en nucleótidos G+C y su pared celular tiene un alto contenido de ácidos micólicos. Como característica especie-específi ca destacan su dependencia del sideróforo micobactina para el crecimiento in vitro y la presencia del segmento de inserción IS900 en su genoma, que se ha utilizado para la detección específi ca de este patógeno 1,5 .MAP es capaz de infectar una amplia variedad

show abstract

Identification of Mycobacterium avium subsp. paratuberculosis in Biopsy Specimens from Patients with Crohn's Disease Identified by In Situ Hybridization

Cited by 127 publications

References 18 publications

Cell Wall Deficiency in Mycobacteria: Latency and Persistence

Cell Wall Deficiency in Mycobacteria: Latency and Persistence

Molecular Characterization of Mycobacterial Ribonucleotide Reductase (RNR) and its Implication as a Novel Drug Target

Mycobacterium avium subsp paratuberculosis y enfermedad de Crohn: evidencias de una zoonosis

Contact Info

Product

Resources

About