Identification of infection by Chikungunya, Zika, and Dengue in an area of the Peruvian coast. Molecular diagnosis and clinical characteristics

Sánchez-Carbonel, José; Tantaléan-Yépez, Derek; Aguilar-Luis, Miguel Ángel; Silva-Caso, Wilmer; Weilg, Pablo; Vasquez-Achaya, Fernando; Costa, Luciana Scarlazzari; Martins-Luna, Johanna; Sandoval, Isabel; Valle-Mendoza, Juana del

doi:10.1186/s13104-018-3290-0

Cited by 16 publications

(21 citation statements)

References 16 publications

(21 reference statements)

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“…In the case of LAMP assays for ZIKV detection, it is important that analytical specificity be assessed in the presence of other mosquito-borne arboviruses found in the region. In the case of the most recent ZIKV outbreak in Latin America, this would include the arboviruses DENV 1-4, CHIKV, and YFV [87][88][89][90][91].…”

Section: Lamp Specificitymentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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The recent outbreak of Zika virus (ZIKV) in the Americas and its devastating developmental and neurological manifestations has prompted the development of field-based diagnostics that are rapid, reliable, handheld, specific, sensitive, and inexpensive. The gold standard molecular method for lab-based diagnosis of ZIKV, from either patient samples or insect vectors, is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The method, however, is costly and requires lab-based equipment and expertise, which severely limits its use as a point-of-care (POC) tool in resource-poor settings. Moreover, given the lack of antivirals or approved vaccines for ZIKV infection, a POC diagnostic test is urgently needed for the early detection of new outbreaks and to adequately manage patients. Loop-mediated isothermal amplification (LAMP) is a compelling alternative to RT-qPCR for ZIKV and other arboviruses. This low-cost molecular system can be freeze-dried for distribution and exhibits high specificity, sensitivity, and efficiency. A growing body of evidence suggests that LAMP assays can provide greater accessibility to much-needed diagnostics for ZIKV infections, especially in developing countries where the ZIKV is now endemic. This review summarizes the different LAMP methods that have been developed for the virus and summarizes their features, advantages, and limitations.Viruses 2020, 12, 19 2 of 20 raising concerns about the neurological tropism of the virus [12]. In May 2015, the first case of ZIKV infection was reported in Brazil [13] and the virus rapidly spread throughout the country and much of Latin America, causing the largest recorded epidemic of the virus to date [14]. The Brazilian epidemic raised great international concern because of severe birth defects, including microcephaly, in neonates born to mothers infected by ZIKV during pregnancy [3,15,16].ZIKV is transmitted mainly through the bite of infected mosquitoes from the genus Aedes, although other vectors may also be involved in the transmission [17][18][19][20][21]. Additionally, other routes of ZIKV transmission have been identified, including blood transfusions, transplacental, perinatal, and sexual intercourse [22][23][24]. ZIKV infection usually causes a self-limiting and a mild illness, where the majority of cases are asymptomatic and, when present, symptoms include fever, headache, rash, conjunctivitis, and arthralgia [25]. In regions where there is a circulation of other arboviruses, such as DENV and chikungunya (CHIKV), the clinical diagnosis of ZIKV infection becomes extremely difficult because of common symptoms. Therefore, laboratory-based molecular diagnosis is of fundamental importance to correctly identify the etiologic agent [3,26].Given the lack of approved vaccines and antivirals against ZIKV, a rapid and reliable point-of-care (POC) diagnostic test for detection of ZIKV is urgently required for control and prevention measures and to increase the diagnostic capacity of ZIKV-affected, mainly in low-resource areas [27,28]. Z...

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Section: Lamp Specificitymentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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“…Clinical diagnosis does not reliably differentiate CHIKV infections from other etiologies on the differential, such as other alphaviruses (MAYV, ONNV), flaviviruses (most commonly DENV and ZIKV), and nonviral pathogens, which include, but are not limited to, Plasmodium species, Leptospira, rickettsia, and Salmonella. In settings of CHIKV cotransmission with DENV and ZIKV, a presumptive diagnosis of chikungunya was correct in only 10 to 40% of cases (7,8,31). Clinical prediction algorithms have been evaluated to differentiate CHIKV infections from infections with other etiologies, but these have not been independently validated in separate cohorts (59,63,66,72,76).…”

Section: Acute Clinical Presentation and Differential Diagnosismentioning

confidence: 99%

“…Attack rates have been relatively low in temperate regions (24), but rates as high as 50 to 75% have been reported during outbreaks in the tropics (25)(26)(27). Such high attack rates help explain the occurrence of human coinfections with CHIKV, DENV, and/or ZIKV, which have been reported from the Americas and Asia (7,(28)(29)(30)(31)(32)(33), and individuals with these coinfections may present with more severe manifestations (7,30). A meta-analysis of available publications on CHIKV coinfections revealed that CHIKV-DENV coinfections were most common (44/109 studies), but CHIKV-malaria coinfections were reported in several studies (5/109) (28,29,34).…”

mentioning

confidence: 99%

Beyond Fever and Pain: Diagnostic Methods for Chikungunya Virus

2019

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Chikungunya virus (CHIKV) is an alphavirus that is primarily transmitted by Aedes species mosquitoes. Though reports of an illness consistent with chikungunya date back over 200 years, CHIKV only gained worldwide attention during a massive pandemic that began in East Africa in 2004. Chikungunya, the clinical illness caused by CHIKV, is characterized by a rapid onset of high fever and debilitating joint pain, though in practice, etiologic confirmation of CHIKV requires the availability and use of specific laboratory diagnostics. Similar to infections caused by other arboviruses, CHIKV infections are most commonly detected with a combination of molecular and serological methods, though cell culture and antigen detection are reported. This review provides an overview of available CHIKV diagnostics and highlights aspects of basic virology and epidemiology that pertain to viral detection. Although the number of chikungunya cases has decreased since 2014, CHIKV has become endemic in countries across the tropics and will continue to cause sporadic outbreaks in naive individuals. Consistent access to accurate diagnostics is needed to detect individual cases and initiate timely responses to new outbreaks.

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“…In Peru situation is similar, studies have reported the presence of the four serotypes of DENV in the Peruvian coast, being the serotype DENV 2 the most frequent during the period from May to August (i.e., in 2016), as well as the Zika virus (ZIKV) and the Chikungunya virus (CHIKV) [7]. In the last 3 years, the presence of the Oropouche virus (OROV) has been reported, reemerging in some areas such as Madre de Dios or recently appearing in other areas such as Huánuco [8,9].…”

Section: Introductionmentioning

confidence: 87%

“…In the first study, 496 samples from patients with Acute Febrile DiseaseIllness (AFI) were analyzed and DENV, CHIKV, or ZIKV were identified in 46.8% (232/496) of the samples. The current study was performed including the total number (496 samples) of patients who met the criteria of AFI (axillary temperature ≥ 38 °C for less than 7 days together with one or more the signs and/or symptoms associated with arbovirus infections described in previous works and developed later in the text [7,13]. We excluded samples in inadequate state of conservation, samples without codification, improperly filled data sheets and those with incomplete demographic data such as age, gender, place of origin, etc.…”

Section: Patients and Samplingmentioning

confidence: 99%

Oropouche infection a neglected arbovirus in patients with acute febrile illness from the Peruvian coast

et al. 2020

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Objective: To evaluate the frequency of infection caused by the Oropouche virus (OROV) in 496 patients with acute febrile disease (AFI), whose samples were obtained for the analysis of endemic arboviruses in a previous investigation carried out in 2016. Results: OROV was detected in 26.4% (131/496) of serum samples from patients with AFI. Co-infections with Dengue virus (7.3%), Zika virus (1.8%) and Chikungunya (0.2%) were observed. The most common clinical symptoms reported among the patients with OROV infections were headache 85.5% (112/131), myalgia 80.9% (106/131), arthralgia 72.5% (95/131) and loss of appetite 67.9% (89/131). Headache and myalgia were predominant in all age groups. Both OROV infections and co-infections were more frequent in May, June and July corresponding to the dry season of the region.

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Identification of infection by Chikungunya, Zika, and Dengue in an area of the Peruvian coast. Molecular diagnosis and clinical characteristics

Cited by 16 publications

References 16 publications

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Beyond Fever and Pain: Diagnostic Methods for Chikungunya Virus

Oropouche infection a neglected arbovirus in patients with acute febrile illness from the Peruvian coast

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