Identification of members of the Pasteurellaceae isolated from birds and characterization of two new taxa isolated from psittacine birds

Bisgaard, Magne; Hinz, K.‐H.; Petersen, Kamille Dumong; Christensen, Jens Peter

doi:10.1080/03079459994632

Cited by 13 publications

(11 citation statements)

References 22 publications

(34 reference statements)

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“…2003). Having been isolated from most orders of birds examined taxa associated with birds seem to dominate (Bisgaard 1993; Bisgaard et al. 1999).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis by 16S rDNA gene sequence comparison of avian taxa of Bisgaard and characterization and description of two new taxa of Pasteurellaceae

Christensen¹,

Foster²,

Jp³

et al. 2003

J Appl Microbiol

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Aims: Characterization and classification of members of Pasteurellaceae isolated from birds by extended phenotypic characterization and 16S rDNA gene sequence comparison. Methods and Results: A total of 95 avian isolates were subjected to extended phenotypic characterization. Thirteen bacterial strains selected from main phenotypic clusters and isolated from parrot, parakeet, budgerigar, partridge, pheasant, chicken, duck, hawk and gull were subsequently characterized by 16S rDNA gene sequencing. Eight of the sequenced strains were classified with six taxa of Bisgaard of which two (34 and 40) have not been published before, and the properties of four others (14, 22, 26 and 32) changed upon the characterization of these new isolates.Of the remaining strains, one was identified as a phenotypic variant in maltose and dextrin of Pasteurella gallinarum another as a trehalose positive variant of taxon 3 of Bisgaard. The remaining three strains sequenced were not closely related to existing taxa of Pasteurellaceae. However, they were found to belong to the Avian cluster with 92-97% 16S rDNA gene sequence similarity. Conclusion:The study allowed the classification of bacteria isolated from birds by the integrated use of extended phenotypic characterization and 16S rDNA gene sequence analysis. Only the application of 16S rDNA gene sequencing allows a correct identification of variant strains. Significance and Impact of the Study: The description of new taxa within the bacterial family Pasteurellaceae will subsequently allow additional isolates of these taxa to be identified and improve the diagnosis and epidemiological understanding of bacteria causing disease in birds.

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“…2003). Having been isolated from most orders of birds examined taxa associated with birds seem to dominate (Bisgaard 1993; Bisgaard et al. 1999).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis by 16S rDNA gene sequence comparison of avian taxa of Bisgaard and characterization and description of two new taxa of Pasteurellaceae

Christensen¹,

Foster²,

Jp³

et al. 2003

J Appl Microbiol

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“…Sequencing of the 16S rRNA gene of strain TIS12640 showed identity to the reference strain of taxon 44, while the highest similarity to other Pasteurellaceae was 96AE9% with another unnamed Bisgaard taxon 37 (strain 111, accession no. AF487721) (Bisgaard et al 1999). A low similarity of only 89% 16S rRNA sequence similarity with other members of Pasteurellaceae (highest to Pasteurella langaaensis) including the isolates characterized in the current investigation underlines that taxon 44 represents a new genus and species, probably belonging to Pasteurellaceae.…”

Section: Sequence Comparison and Phylogenetic Analysismentioning

confidence: 69%

“…Sequencing of the 16S rRNA gene of strain TIS12640 showed identity to the reference strain of taxon 44, while the highest similarity to other Pasteurellaceae was 96·9% with another unnamed Bisgaard taxon 37 (strain 111, accession no. ) (Bisgaard et al. 1999).…”

Section: Resultsmentioning

confidence: 99%

“…Bisgaard et al. (1999) reviewed the prevalence and significance of Pasteurellaceae in Psittaciformes and stressed that a more accurate basis for diagnosis of these organisms is a prerequisite for improving our knowledge and understanding of the complexity of factors that govern the ecological preferences and virulence factors of these organisms.…”

Section: Introductionmentioning

confidence: 99%

“…Little knowledge, however, exists as to the prevalence of the newly reported taxa among confined psittacine hosts . Bisgaard et al (1999) reviewed the prevalence and significance of Pasteurellaceae in Psittaciformes and stressed that a more accurate basis for diagnosis of these organisms is a prerequisite for improving our knowledge and understanding of the complexity of factors that govern the ecological preferences and virulence factors of these organisms. The gene rpoB encodes the b-subunit of DNA-dependent RNA-polymerase, and rpoB gene sequence-based classification was used with Pasteurellaceae by Angen et al (2003) and Korczak et al (2004).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Pasteurellaceae-like bacteria isolated from clinically affected psittacine birds

Gregersen

Neubauer

Korczak

et al. 2010

Journal of Applied Microbiology

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Aims: The aim of the present investigation was to identify and characterize Pasteurella‐like isolates obtained from clinically affected psittacine birds. Methods and Results: A total of 37 isolates from psittacine birds tentatively classified with the family Pasteurellaceae were characterized phenotypically. The genetic relationship was investigated by sequencing of partial rpoB and 16S rRNA genes for selected isolates. The results obtained were compared with the data from 16 reference strains. Nine isolates were identified as Gallibacterium spp., 16 as Volucribacter spp. or Volucribacter‐like, while 11 isolates were classified as taxon 44 of Bisgaard. A single isolate was identified as Pasteurella multocida. Conclusions: Characterization of Pasteurellaceae by traditional methods is often inconclusive because of inconsistent reactions and phenotypic diversity. For the same reason, genotyping is essential to allow proper classification as demonstrated in the present study. Significance and Impact of the Study: Limited information exists on the isolation and significance of Pasteurellaceae associated with clinically affected psittacine birds showing signs of digestive and/or respiratory disorders. The present investigations demonstrated that these organisms are widely distributed among clinically affected birds, but isolation of these taxa cannot be unambiguously correlated with the symptoms observed.

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Pasteurella

Mutters¹,

Bisgaard²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Pas.teu.rel' la . M.L. dim. fem. n. Pasteurella named after Louis Pasteur. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Pasteurella Coccobacilli or rods , generally, 0.3–1.0 × 1.0–2.0 µm. Depending on the growth stage, cells occur singly, in pairs, or less frequently in short chains. Sometimes threads or filaments are formed resulting in marked pleomorphism. Pleomorphism occurs mainly in old cultures. Gram negative , although bipolar staining often can be observed . In tissues, P. multocida often shows bipolar staining with Giemsa or Wright's stain. Not acid fast. Endospores are not formed. Nonmotile. Aerobic to microaerophilic or facultatively anaerobic . Chemoorganotrophic with both oxidative and fermentative types of metabolism. Electron transport system is cytochrome‐based with oxygen, nitrate, or fumarate as the terminal electron acceptor. Nitrate reductase is produced. Oxidase positive, alkaline phosphatase positive , and almost always catalase positive. Most species are V‐factor and X‐factor independent, but V‐factor‐requiring strains do occur . Even after specific growth factors have been provided, complex media may be required to obtain better growth . Optimum growth temperature 35–37°C. Arginine dihydrolase and lysine decarboxylase negative. Gelatin is not liquefied within 48 h. No growth occurs in Simmons citrate medium. Acid is produced from D ‐glucose, D ‐galactose, D ‐fructose, D ‐mannose, and sucrose. Acid is not produced from L ‐sorbose, L ‐rhamnose, m ‐inositol, and adonitol. Parasitic in vertebrates, particularly mammals and birds. Genome molecular weights range from 1.4 × 10 9 to 1.9 × 10 9 . The mol % G + C of the DNA is : 37.7–45.9. Type species : Pasteurella multocida (Lehmann and Neumann 1899) Rosenbusch and Merchant 1939, 85 ( Bacterium multocidum multocidum (sic) Lehmann and Neumann 1899, 196; Pasteurella gallicida (Burrill 1883) Buchanan 1925, 414.)

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Identification of members of the Pasteurellaceae isolated from birds and characterization of two new taxa isolated from psittacine birds

Cited by 13 publications

References 22 publications

Phylogenetic analysis by 16S rDNA gene sequence comparison of avian taxa of Bisgaard and characterization and description of two new taxa of Pasteurellaceae

Phylogenetic analysis by 16S rDNA gene sequence comparison of avian taxa of Bisgaard and characterization and description of two new taxa of Pasteurellaceae

Characterization of Pasteurellaceae-like bacteria isolated from clinically affected psittacine birds

Pasteurella

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