Identification of Methylobacterium strains using sequence analysis of 16S rRNA genes

Romanovskaya, V. A.; Rokitko, P. V.; Shi-lin, SU; Chernaya, N. A.; Malashenko, Yu. R.

doi:10.1007/s11021-005-0016-9

Cited by 2 publications

(5 citation statements)

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“…KLDS1.0318 genomic DNA was isolated using the DNeasy Tissue kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The universal primer (27F/1492R) was used for PCR and the amplification conditions were as follows: initial denaturation step at 94°C for 3 min, 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, elongation at 72°C for 1 min and final extension at 72°C for 5 min (Romanovskaya et al, 2004). The PCR product was then sent for sequencing to the Comate Bioscience Co., Ltd (Jilin, China).…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus plantarum KLDS1.0318 Ameliorates Impaired Intestinal Immunity and Metabolic Disorders in Cyclophosphamide-Treated Mice

Meng

Wang

et al. 2019

Front. Microbiol.

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Cyclophosphamide (CTX), a clinically important antineoplastic drug, also leads to some side effects such as nausea, vomiting and diarrhea in the consumer. In this study, Lactobacillus plantarum ( L. plantarum ) KLDS1.0318 preserved in our laboratory was orally administered to CTX-treated mice to explore its potential effects to attenuate the toxic effects of CTX-induced by modulating intestinal immune response, promoting intestinal integrity and improving metabolic profile. BALB/c mice were randomly divided into six groups including normal control group (NC; non-CTX with sterile saline), model control group (MC; CTX-treated with sterile saline), CTX-treated with L. plantarum KLDS1.0318 (10 mL/kg) groups with three different doses (KLDS1.0318-L, 5 × 10 7 CFU/mL; KLDS1.0318-M, 5 × 10 8 CFU/mL; KLDS1.0318-H, 5 × 10 9 CFU/mL), and CTX-treated with levamisole hydrochloride (40 mg/kg) as a positive control (PC) group. After receiving the bacterium for 20 days, samples of small intestine and colonic contents were collected for different analyses. The results revealed that the levels of cytokines secreted by Th1 cells (IL-2, IFN-γ, and TNF-α) and Th2 cells (IL-4, IL-6, and IL-10) in probiotic treatment groups were significantly higher than those in the MC group. Histopathological results showed that L. plantarum KLDS1.0318 favorably recovered CTX-induced abnormal intestinal morphology by improving the villus height and crypt depth as well as quantity of goblet cells and mucins production. Compared to CTX alone-treated group, the production of short-chain fatty acids (SCFAs) were significantly increased and the levels of pH and ammonia were decreased significantly with high dose L. plantarum KLDS1.0318 supplementation. Compared with mice in CTX alone-treated group, mice in three groups of KLDS1.0318 had increased Bifidobacterium and Lactobacillus and decreased Escherichia and Enterococcus in their cecal content. The present findings suggested that L. plantarum KLDS1.0318 could be of significant advantage to mitigate the harmful effects of CTX and improve the intestinal health in mice.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus plantarum KLDS1.0318 Ameliorates Impaired Intestinal Immunity and Metabolic Disorders in Cyclophosphamide-Treated Mice

Meng

Wang

et al. 2019

Front. Microbiol.

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“…Ltd) и агаризованное сусло. Различные экологофизиологические группы микроорганизмов выделяли на селективных средах [6], метилотрофные бактерии -на минеральной среде с метанолом (0.5%) [18].…”

Section: материалы и методыunclassified

“…Идентификацию бактерий проводили на основании изучения их морфологофизиологических свойств [6], а также используя сиквенс-анализ генов 16S рРНК. Методами, описанными в [18], из биомассы антарктических бактерий выделяли ДНК. С использованием полимеразной цепной реакции (ПЦР) получали амплифицированные гены 16S рРНК и их секвенировали.…”

Section: материалы и методыunclassified

“…Ltd) and wort agar medium. Ecological and physiological groups of microorganisms isolated on selective media [6], methylotrophic bacteria -on methanolcontaining (0.5 %) mineral agar medium [18].…”

Section: Isolation Of Antarctic Microorganismsmentioning

confidence: 99%

“…Bacteria identification was carried out by studying morphological and physiological properties using standard methods [6], and also 16S rRNA gene sequence. With the methods described in [18], we extracted DNA from a biomass of Antarctic bacteria. Using polymerase chain reaction (PCR) amplified 16S rRNA genes were sequenced.…”

Section: Isolation Of Antarctic Microorganismsmentioning

confidence: 99%

See 2 more Smart Citations

Trends and prospects for developing of new industrial biotechnologies on the base of Antarctic extremophilic microorganisns

Tashyrev

Romanovskaya

Берегова

et al. 2010

UAJ

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БІОЛОГІЧНІ ДОСЛІДЖЕННЯ BIOLOGICAL RESEARCH Резюме. Цель работы-изучение антарктических экстремофильных микроорганизмы, а также оценка перспектив их использования для создания новых природоохранных биотехнологий и медицинских препаратов, селекции продуцентов биологически активных веществ. Системное изучение структуры и функций микробных ценозов в Антарктике показало, что их адаптация проявляется в виде гомеостаза, т.е. сохранении жизнеспособности и способности к росту в широком диапазоне концентраций экстремальных факторов. Создана коллекция микроорганизмов, устойчивых к этим факторам. Коллекция включает микроорганизмы, синтезирующие

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Identification of Methylobacterium strains using sequence analysis of 16S rRNA genes

Cited by 2 publications

References 1 publication

Lactobacillus plantarum KLDS1.0318 Ameliorates Impaired Intestinal Immunity and Metabolic Disorders in Cyclophosphamide-Treated Mice

Lactobacillus plantarum KLDS1.0318 Ameliorates Impaired Intestinal Immunity and Metabolic Disorders in Cyclophosphamide-Treated Mice

Trends and prospects for developing of new industrial biotechnologies on the base of Antarctic extremophilic microorganisns

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