Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

Lloyd, Charmaine; Tan, Kai Heng; Lim, Kar Leong; Valu, Vimala Gana; Fun, Sarah Mei Ying; Chye, Teng Rong; Mak, Hui Min; Sim, Wei Xiong; Musa, Sarah Liyana; Ng, Joscelyn Jun Quan; Nordin, Nazurah Syazana Bte; Aidzil, Nurhazlyn Bte Md; Eng, Zephyr Yu Wen; Manickavasagam, Punithavathy; New, Jen Yan

doi:10.1038/s41598-021-84112-0

Cited by 13 publications

(4 citation statements)

References 45 publications

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“…20 different microalgal strains were isolated from freshwater resources in Pakistan by serial dilution and streak plating and cultured in BBM for maintenance [22]. In a similar study, 94 culturable pure isolates of microalgae were obtained through plating and serial dilution techniques from freshwater sources of Singapore city and maintained in BBM for further characterization [23].…”

Section: Sample Collection Isolation and Culturementioning

confidence: 99%

Poly-unsaturated Fatty Acid, Biodiesel Property and Anticancer Activity Analysis of Monoraphidium griffithii

Vinitha

Meignanalakshmi²,

Tirumurugaan³

et al. 2022

CJAST

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Aim: The aim of the study was to isolate and characterize freshwater microalgae from Puzhal lake, Chennai, Tamil Nadu, and to analyze its biochemical composition, poly-unsaturated fatty acid content, anticancer activity, cytotoxicity, and biodiesel properties. Methodology: Pure isolate obtained from freshwater was characterised biochemically and identified by ITS-2 RNA sequence as Monoraphidium griffithii. The microalgal culture was maintained under white light illumination for a photoperiod of 16:8 light/dark hours respectively. The saturated and poly-unsaturated fatty acid profiling was performed in a Gas Chromatography-Flame ionization detector and the biodiesel properties were analyzed through BiodieselAnalyzer© Ver. 2.2 software. The anti-cancer and cytotoxicity studies were performed using the human lung cancer cell line (A549) and Vero cell line at 20, 40, 60, 80, and 100 µg biomass/ml concentration. Results: The biochemical composition was determined to be 32.97%±1.648 protein, 15.36%±2.857 carbohydrate, 15.89%±1.407 lipid, 26.27±1.48 µg/mg chlorophyll a+b and 8.03±1.92 µg/mg carotenoid. The fatty acid profiling revealed the presence of 19 different fatty acids of which Palmitic acid was 18.08%, cis-10 heptadecanoic acid was 17.37%, alpha-linolenic acid was 2.05%, and docosahexaenoic acid was 1.27%. The biodiesel properties were within the American standard for testing materials (ASTM) limits. 46.5% cell viability was attained at a microalgal concentration of 80 µg/ml against the human lung cancer cell line (A549) and the cell viability was 77.87% even at the high concentration of 100 µg/ml against the Vero cell line. Conclusion: The isolated microalgae Monoraphidium griffithii can be used for the production of biodiesel. The presence of alpha-linolenic and docosahexaenoic acid and anticancer activity makes them a source for human and animal nutrition.

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Section: Sample Collection Isolation and Culturementioning

confidence: 99%

Poly-unsaturated Fatty Acid, Biodiesel Property and Anticancer Activity Analysis of Monoraphidium griffithii

Vinitha

Meignanalakshmi²,

Tirumurugaan³

et al. 2022

CJAST

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“…In this medium, we were able to isolate thirty different species of green algae. We recognize that some genera are difficult to cultivate in this medium, thus we claim that our study is representative of Gujar Khan's overall green algal biodiversity (Lloyd et al, 2021). The study revealed that sampling areas with varied ecological variables play important role in influencing the distribution and diversity of subaerial algal communities.…”

Section: Cosmarium Crenatummentioning

confidence: 87%

A Prospective Study on Morphological Identification and Characterization of Freshwater Green Algae Based on the Microscopic Technique in District Rawalpindi

Minhas¹,

Mumtaz²,

Kaleem³

et al. 2023

PJAR

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G reen algae are one of the most diverse groups of algae, with at least 7000 species (Nelson and Garcia-Pichel, 2021). Green algae are characterized by several distinct features including filaments, colonies, branched, motile, non-motile, and bladelike thallus (Leliaert et al., 2012). Chloroplast contained chlorophyll a, b and accessory pigments including carotenes and xanthophylls which are surrounded by double membrane with thylakoid arranged in lamellae. Pyrenoid are present and embedded in the chloroplast and surrounded by starch, the primary reserve carbohydrate (Forjan et al., 2015). Green algae have variations in their morphology ranging from microscopic flagellated unicells to complex macroscopic thalli with varying degrees of morphological differentiation. They are a paraphyletic group from a taxonomic standpoint since they have a common ancestor with plants and have Abstract | The current study was carried out to isolate, identify and characterized fresh water green algae species from ecologically diverse habitats of Tehsil Gujar Khan, District Rawalpindi. A microscopic image data was used to identify algal species. A total of 30 species were recorded that belonged to 4 orders, 11 families, and 14 genera. Clamydomonas reinardtii, Acutodesmus obliquus and Cosmarium isthmocondrum are new record from Pakistan. Among identified taxa, Scenedesmus was the dominant genus with 8 species and their contribution was 26.6%. The 2 nd most dominant genus was Cosmarium with 6 species and their contribution was 20%. The other genera Chlorococcum, Ankistrodesmus, Coelastrum, and Closterium were (6.6%). Some genera represented only one (3.3%) species that were Chlamydomonas, Eudorina, Tetraspora, Chlorella, Westella, Pediastrum, Acutodesmus, and Stigeoclonium. Hence, the current study reveals that Gujar Khan, District Rawalpindi is a rich source of green algae and an ideal place for their cultivation.

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“…The cultures from the third sampling showed an abundance of Chlorella with a dispersed presence of diatoms. Although microalgae are unique to ecological sites of isolation (Lloyd et al, 2021), previous surveys of microalgal genera showed the high domination potentials of Chlorella (Lloyd et al, 2021;Palmer, 1974as cited by Abdel-Raouf, 2012Severes, 2018). However, the varying results may be attributable to the local geographical, climatic, and ecological conditions (Ramachandra et al, 2011).…”

Section: Comparison Of Microalgal Assemblage On Different Sampling Datesmentioning

confidence: 98%

Bioremediation by Chlorella vulgaris: Potentials for Treatment of Municipal, Agricultural, and Industrial Wastewater Sources

Alberca¹,

Arbilo²,

Panaglima³

et al. 2023

Preprint

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Developing countries such as the Philippines suffer from a lack of policy development and implementation on wastewater treatment and discharge. Chlorella vulgaris is a microscopic green algae that has been employed in other countries for WWT due to its ability to simultaneously reduce pollutants and produce valuable biomass. However, challenges in technology adaptation such as differential efficiency depending on the location and wastewater types were encountered. Three liquid wastes of different origins—municipal (public market), agricultural (piggery effluent), and industrial (meat processing plant) wastewaters, were used to gauge the remediation potentials of C. vulgaris in a simple photobioreactor setup. Treated samples from municipal, agricultural, and industrial wastewaters showed microalgal growth rates of 0.2685, 0.1527, and 0.1809, respectively, along the 6-day treatment period. Post-intervention comparisons of treated vs. untreated samples revealed a lower electrical conductivity, total dissolved solids, chemical oxygen demand, nutrients (nitrate, ammonia, phosphate), and fecal coliform (MPN/100 mL) on treated samples. Moreover, all treated samples demonstrated relatively higher dissolved oxygen concentrations, denoting the photosynthetic activity by the microalgae. Therefore, Chlorella vulgaris could be harnessed for the remediation of different wastewaters in Nagcarlan, Laguna, Philippines to circumvent issues in water reclamation and degradation.

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Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

Cited by 13 publications

References 45 publications

Poly-unsaturated Fatty Acid, Biodiesel Property and Anticancer Activity Analysis of Monoraphidium griffithii

Poly-unsaturated Fatty Acid, Biodiesel Property and Anticancer Activity Analysis of Monoraphidium griffithii

A Prospective Study on Morphological Identification and Characterization of Freshwater Green Algae Based on the Microscopic Technique in District Rawalpindi

Bioremediation by Chlorella vulgaris: Potentials for Treatment of Municipal, Agricultural, and Industrial Wastewater Sources

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