Identification of NbME MITE families: Potential molecular markers in the microsporidia Nosema bombycis

Xu, Jianlong; Wang, Min; Zhang, Xiaoyan; Tang, Fahui; Pan, Guoqing; Zhou, Zeyang

doi:10.1016/j.jip.2009.10.011

Cited by 23 publications

(32 citation statements)

References 25 publications

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“…DWV is the principal Varroa transmitted virus and usually the immediate cause of Varroa mite – associated colony mortality [12]. As expected, our study showed a strong positive correlation between DWV titres and Varroa infestation rates, as well as an explanatory effect of mite infestation rates on DWV titres in the statistical analysis.…”

Section: Discussionsupporting

confidence: 87%

“…However, Varroa -induced colony collapse is more accurately a result of secondary virus infections vectored by the mite that precipitates a progressive epidemic and ultimately colony mortality [8], [9], [10]. Although initially obscure and practically unknown, deformed wing virus (DWV) has become one of the most prevalent viruses world-wide due to its close association with Varroa mite infestation [11], [12]. Varroa -mediated virus transmission during pupal development is directly responsible for the characteristic wing deformities resulting in flightless adults that die shortly after emerging [12], [13].…”

Section: Introductionmentioning

confidence: 99%

“…Although initially obscure and practically unknown, deformed wing virus (DWV) has become one of the most prevalent viruses world-wide due to its close association with Varroa mite infestation [11], [12]. Varroa -mediated virus transmission during pupal development is directly responsible for the characteristic wing deformities resulting in flightless adults that die shortly after emerging [12], [13].…”

Section: Introductionmentioning

confidence: 99%

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Increased Tolerance and Resistance to Virus Infections: A Possible Factor in the Survival of Varroa destructor-Resistant Honey Bees (Apis mellifera)

2014

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The honey bee ectoparasitic mite, Varroa destructor, has a world-wide distribution and inflicts more damage than all other known apicultural diseases. However, Varroa-induced colony mortality is more accurately a result of secondary virus infections vectored by the mite. This means that honey bee resistance to Varroa may include resistance or tolerance to virus infections. The aim of this study was to see if this is the case for a unique population of mite-resistant (MR) European honey bees on the island of Gotland, Sweden. This population has survived uncontrolled mite infestation for over a decade, developing specific mite-related resistance traits to do so. Using RT-qPCR techniques, we monitored late season virus infections, Varroa mite infestation and honey bee colony population dynamics in the Gotland MR population and compared this to mite-susceptible (MS) colonies in a close by apiary. From summer to autumn the deformed wing virus (DWV) titres increased similarly between the MR and MS populations, while the black queen cell virus (BQCV) and sacbrood virus (SBV) titres decreased substantially in the MR population compared to the MS population by several orders of magnitude. The MR colonies all survived the following winter with high mite infestation, high DWV infection, small colony size and low proportions of autumn brood, while the MS colonies all perished. Possible explanations for these changes in virus titres and their relevance to Varroa resistance and colony winter survival are discussed.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Increased Tolerance and Resistance to Virus Infections: A Possible Factor in the Survival of Varroa destructor-Resistant Honey Bees (Apis mellifera)

2014

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“…Due to a strongly AT-biased genome (74 % A+T) and diversity of repetitive elements in microsporidia, assembly is a complicated process (Cornman et al 2009). The assembled frame genome of N. bombycis has been shown to be dispersive and incomplete using high-throughput sequencing (Xu et al 2010). Further, fine mapping can be done with the aid of the BCA library (Yang et al 2009).…”

Section: Cdna Library Construction and Est Analysismentioning

confidence: 99%

Morphological and molecular characterization of Nosema pernyi, a microsporidian parasite in Antheraea pernyi

et al. 2015

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Nosema pernyi is a lethal pathogen that causes microsporidiosis in the Chinese oak silkworm, Antheraea pernyi. In this study, we presented its morphological and some molecular characteristics. The mature spores were measured to be 4.36 × 1.49 μm. The spore wall consisted of an electron-dense exospore (EX) and electron-lucent endospore (EN) layer. The polar filament (PF) was isofilar with 10-12 coils that were frequently arranged in a single row. Investigation results indicated that N. pernyi can infect the gut wall, silk glands, and other tissues. A full-length SMART cDNA library of N. pernyi was constructed, and then 824 expressed sequence tags (ESTs) were sequenced. Ninety unigenes, out of 197 assembled unigenes, showed significant homology to known genes of Nosema ceranae, Nosema bombycis, Encephalitozoon cuniculi, and other microsporidian species. Based on the nucleotide sequence of the α- and β-tubulin genes and amino acid sequence of actin gene, phylogenetic trees analysis showed that N. pernyi was closely related to Nosema philosamiae and Nosema antheraeae. It was correctly assigned to the Nosema group.

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“…Descriptions in other organisms, such as bacteria (Chen et al, 2008), cyanobacteria , fungi (Xu et al, 2010), silkworms (Han et al, 2010), fish (de Boer et al, 2007) and amphibians (Hikosaka et al, 2011) are also found in the literature, but few occurrences have been reported in the Drosophila genus (Tudor et al, 1992 ;Miller et al, 2000 ;Ortiz et al, 2010). Although numerous MITEs have been identified, the association with autonomous elements is often absent.…”

Section: Introductionmentioning

confidence: 98%

msechBari, a new MITE-like element inDrosophila sechelliarelated to theBaritransposon

Dias

Carareto

2011

Genet. Res.

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SummaryA few occurrences of miniature inverted-repeat transposable elements (MITEs) have been reported in species of the genus Drosophila. Here, we describe msechBari, a MITE-like element in Drosophila sechellia. The element is short, approximately 90 bp in length, AT-rich and occurs in association with, or close to, genes, characteristics that are typical for MITEs. The identification was performed in silico using the sequenced genome of D. sechellia and confirmed in a laboratory strain. This short element is related to the Bari_DM transposon of Drosophila melanogaster, having terminal inverted repeats (TIRs) of a similar length and a high identity with the full-length Bari_DM element. The estimated recent origin of the element and the homogeneity observed between copies found in the genome suggests that msechBari could be active in D. sechellia.

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Identification of NbME MITE families: Potential molecular markers in the microsporidia Nosema bombycis

Cited by 23 publications

References 25 publications

Increased Tolerance and Resistance to Virus Infections: A Possible Factor in the Survival of Varroa destructor-Resistant Honey Bees (Apis mellifera)

Increased Tolerance and Resistance to Virus Infections: A Possible Factor in the Survival of Varroa destructor-Resistant Honey Bees (Apis mellifera)

Morphological and molecular characterization of Nosema pernyi, a microsporidian parasite in Antheraea pernyi

msechBari, a new MITE-like element inDrosophila sechelliarelated to theBaritransposon

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