Identification of New QTL Contributing to Barley Yellow Dwarf Virus-PAV (BYDV-PAV) Resistance in Wheat

Abstract

Barley yellow dwarf (BYD) is a major virus disease which dramatically reduces wheat yield. Introducing BYD resistance genes into commercial varieties has been proven to be effective in reducing damage caused by barley yellow dwarf virus (BYDV). However, only one major resistance gene is readily deployable for breeding; Bdv2 derived from Thinopyrum intermedium is deployed as a chromosomal translocation. In this study, a double haploid (DH) population was developed from a cross between XuBYDV (introduced from China showing very good resistance to BYD) and H-120 (a BYD-sensitive Chinese accession), and was used to identify QTL for BYD resistance. The population was genotyped using an Infinium iSelect bead chip array targeting 90K gene-based SNPs. The disease resistance of DH lines inoculated with BYDV was assessed at the heading stage. The infections were assessed by tissue blot immunoassay (TBIA). Three new QTL were identified on chromosomes 5A, 6A, and 7A for both symptom and TBIA, with all three resistance alleles being inherited from XuBYDV. Some DH lines with the resistance alleles from all three QTL showed high level resistance to BYD. These new QTL will be useful in breeding programs for pyramiding BYD resistance genes.

Identification of novel QTL contributing to barley yellow mosaic resistance in wild barley (Hordeum vulgare spp. spontaneum)

“…DNA extraction and genotyping were performed as described in previous publications [ 57 ]. Two parental varieties and 154 DH lines were genotyped with DArTSeq ( http://www.diversityarrays.com/ ).…”

Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat

“…To date, most breeding efforts have been directed to the identification of viral tolerance, also known as 'field resistance', that refers to the ability of the plant to yield under BYD infection and is associated with a reduction of symptoms of infection independent of the virus titer (Foresman et al 2016). Field resistance has been reported to be polygenic, falling under the quantitative resistance class, where several genes with very small effects control the resistance response (Qualset et al 1973, Cisar et al 1982Ayala et al 2002;Choudhury et al 2019a;c).…”

“…In addition to the four known resistance genes, other genomic regions associated with BYD resistance have been identified through genetic mapping. These regions have been described on nearly all wheat chromosomes but have not been genetically characterized (Ayala et al 2002;Jarošová et al 2016;Choudhury et al 2019a;b;c). Moreover, two recent studies have reported that some of these new genomic regions display additive effects (Choudhury et al 2019a;b).…”

“…2019a; b; c). Moreover, two recent studies have reported that some of these new genomic regions display additive effects (Choudhury et al . 2019a; b).…”

The Effect of Caffeine and Trifluralin on Chromosome Doubling in Wheat Anther Culture

“…Improvements in wheat DH production have resulted from co-culture with ovaries [11] and n-butanol treatment [12,13]. Approximately 90% of the DH lines developed in our program are destined for plant breeding companies, with the remaining populations used for research projects throughout Australia [14,15]. Genotype variability in key production parameters, such as embryo production, green plant regeneration and chromosome doubling, is an ongoing challenge, especially for the high-throughput production of breeding populations.…”