Identification of new transformation products during enzymatic treatment of tetracycline and erythromycin antibiotics at laboratory scale by an on-line turbulent flow liquid-chromatography coupled to a high resolution mass spectrometer LTQ-Orbitrap

Abstract

33In the case of Tc, the tentative molecular formulas with errors mass within 2 ppm have been 34 based on the hypothesis of dehydroxylation, (bi)demethylation and oxidation of the rings A and 35C as major reactions. In contrast, the major TP detected for ERY has been identified as the 36 "dehydration ERY-A", with the same molecular formula of its parent compound. In addition, the 37 evaluation of the antibiotic activity of the samples along the enzymatic treatments showed a 38 decrease around 100% in both cases.

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

“…This is presumably due to the strong electron donating aromatic amine group in sulfonamides and the phenol group in tetracyclines, which are not found in quinolones (Ding et al, 2016). However, identified tetracycline transformation intermediates suggest that the phenol group is not the primary target for laccase oxidation, and that oxygen addition, demethylation, water elimination reactions occur during laccase treatment (Llorca et al, 2015; Yang et al, 2017). For sulfonamides, increasing electronegativity of the substituents is accompanied by decreased degradation (Yang C. W. et al, 2016).…”

Simultaneous determination of aflatoxin B1, B2, G1, and G2 in corn powder, edible oil, peanut butter, and soy sauce by liquid chromatography with tandem mass spectrometry utilizing turbulent flow chromatography

“…Compared with traditional off‐line SPE, the on‐line TFC column is very effective for direct and fast analysis of complicated samples . The on‐line TFC columns have been used in the determination of tetracycline and erythromycin antibiotics at laboratory scale , bisphenol A and other phenols in urine , polyphenols in jelly products , the metabolomic analysis of human plasma , and anabolic androgenic steroids in urine On‐line SPE has been used in the analysis of pesticides . Although TFC is a mature and commercially available technique and has been utilized for online sample clean‐up in some cases, it is necessary to establish a TFC–LC–MS/MS method for the rapid screening of AFs in real samples.…”

Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1

“…The MS analysis was performed with a heated electrospray ionization (HESI) source in positive mode with a spray voltage of 3.5 kV, S-lens RF level of 50%, and a capillary temperature of 300°C. The MS acquisition was performed in full scan mode 50-1000 Da with a mass resolution of 70000[41,[43][44][45]. The molecular structure for tetracycline and their transformation products were tentatively proposed by the detection of predicted mass, changing in full scan ion intensity, and ring double bound equivalent number (RDB) from the reacted sample…”