Identification of Novel Cytotoxic Peptide KENPVLSLVNGMF from Marine Sponge Xestospongia testudinaria, with Characterization of Stability in Human Serum

Quah, Yixian; Ismail, Nor Ismaliza Mohd; Ooi, Jillian Lean Sim; Amri, Affendi Yang; Manan, Fazilah Abd; Wong, Fai-Chu; Chai, Tsun-Thai

doi:10.1007/s10989-017-9604-6

Cited by 20 publications

(26 citation statements)

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“…The samples were brought back to the laboratory on ice, stored in a freezer at −20 °C, and lyophilized before use. Proteins from S. glaucum were isolated as described by Quah et al (2017). The protein content of the isolate was determined with Bradford's assay (Bradford, 1976), using a standard curve constructed with bovine serum albumin.…”

Section: Preparation Of S Glaucum Protein Isolate and Hydrolysatesmentioning

confidence: 99%

“…Hydrolysis was performed as previously described (Quah et al, 2017). Briefly, the lyophilized protein isolate was dissolved in 50 mmol/L sodium phosphate buffer at a mass (g):volume (mL) ratio of 1:200.…”

Section: Preparation Of S Glaucum Protein Isolate and Hydrolysatesmentioning

confidence: 99%

“…The MTT assay was performed as reported previously (Quah et al, 2017). Briefly, the HeLa human cervical cancer cell line (ATCC CCL-2) was grown at 37 °C in a humidified incubator in 5% CO 2 , in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin.…”

Section: Mtt Assaymentioning

confidence: 99%

“…Separation techniques, such as membrane ultrafiltration (UF), gel filtration (GF) chromatography, fast protein liquid chromatography, and reversed-phase high-performance liquid chromatography (RP-HPLC), are often employed to purify the bioactive peptides before they can be identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) or Edman degradation (Chai et al, 2017). Anticancer peptides have been identified from various marine organisms, including sponges (Quah et al, 2017), tunicates (Jumeri and Kim, 2011), fish (Picot et al, 2006;Hsu et al, 2011;Hung et al, 2014), and oysters (Wang et al, 2010). However, only a few studies have evaluated the cytotoxicity of those peptides on normal or non-cancerous cells to characterize selectivity of the peptides (Chen et al, 2013;Pan et al, 2016;Daliri et al, 2017).…”

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confidence: 99%

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Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Quah

Ismail

Ooi

et al. 2019

J. Zhejiang Univ. Sci. B

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Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC 50) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC 50 , AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs.

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Section: Preparation Of S Glaucum Protein Isolate and Hydrolysatesmentioning

confidence: 99%

Section: Preparation Of S Glaucum Protein Isolate and Hydrolysatesmentioning

confidence: 99%

Section: Mtt Assaymentioning

confidence: 99%

mentioning

confidence: 99%

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Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Quah

Ismail

Ooi

et al. 2019

J. Zhejiang Univ. Sci. B

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“…Ethyl acetate extract of simbiotic fungi from marine sponge Acanthrongylophora ingens also has antibacterial and anti cancer activity (Handayani & Aminah, 2017). Cytotoxic activity on marine sponges of Xestospongia testudinaria by testing on human cervical cancer cells with EC50 0.67 mm (Quah, 2017). Actinomycetes associated with marine sponge Xestospongia sp show activity as antimicrobials (Kim, 2006;Montolvo, 2005;Zhang, 2006) and Aspergillus aculeatus lizuka symbiotic from marine sponge Xestospongia testudinaria have activity as α-glucoside inhibitors ( Ingavat, 2009).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Cytotoxic Activities Screening of Symbiotic Fungi Extract Isolated from Marine Sponge Xestospongia testudinaria DD-01

Bakhtra¹,

Suryani²,

Yuni³

et al. 2019

JCHPS

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Research of antimicrobial activity and cytotoxic activity from isolates symbiotic fungi from marine sponge Xestospongia testudinaria has be done. Xestopongia testudinaria collected from Mande, South Pesisir, West Sumatera. Isolation symbiotic fungi using sabouraud dextrose agar (SDA) as growth medium. Seven isolates symbiotic fungi strains were obtained from this sponge Xestospongia testudinaria. Symbiotic fungi isolate was cultivated using rice medium for 40 day. The cultivation yield was extracted using an ethyl acetate, from the extraction result obtained the weight ethyl acetate extract which varied that 320-756 mg.The ethyl acetate extracts were analyzed for antimicrobial and cytotoxic activities by using diffusion agar method and BSLT. The research revealed 71.42% of the total extract had antimicrobial activity against pathogenic bacteria and fungal such as, Staphylococcus aureus, Pseudmonas aeruginosa and Candida albicans and 71% were cytotoxic activity with Artemia salina L. This selected fungal isolates were first macroscopically and microscopically characterized and later molecularly identified as Penicillium citrinum. This research concluded that the sponge Xestospongia testudinaria symbiotic fungi can be developed as a new source of antibiotic and anticancer compounds.

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Food-Derived Anticancer Peptides: A Review

Sharma

Kaur

Kehinde

et al. 2020

Int J Pept Res Ther

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Cancer has gained appreciable attention in the category of noncommunicable health disorders as the group of proliferative diseases and metabolic syndromes of fatal consequence, globally. The conventional chemotherapeutic, radiological and associated pharmacotherapeutic advances for its cure have timelessly displayed severe aftereffects in patients. Furthermore, the underlying costs and technical requirements of these have added to their limitation recitals. Food-derived bioactive peptides have been scientifically proven as suitable alternatives for cancer management. Studies have shown that some attributes they possess such as specificity, smaller sizes, better ease of syntheses and modification, and improved penetration into cell membranes make them better alternatives to some protein isolates. Their comparative precedencies with regards to natural and efficacy with minimal side effects have offered them the recognition as proficient options in cancer therapy. This review integrates contemporary technical information about bioactive peptides from plants and animal foods and food by-products and their experimentally determined potentials as remedies for cancer. Furthermore, technical details about their isolation methodologies are offered. This article is an augmentation to technical literatures on the cancer subject and an academic exposure of the food-originated approach for its management.

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Identification of Novel Cytotoxic Peptide KENPVLSLVNGMF from Marine Sponge Xestospongia testudinaria, with Characterization of Stability in Human Serum

Cited by 20 publications

References 50 publications

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Antimicrobial and Cytotoxic Activities Screening of Symbiotic Fungi Extract Isolated from Marine Sponge Xestospongia testudinaria DD-01

Food-Derived Anticancer Peptides: A Review

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