Identification of novel rice (Oryza sativa) HPP and HIPP genes tolerant to heavy metal toxicity

Khan, Irfan Ullah; Rono, Justice Kipkoir; Zhang, Bai Qing; Liu, Xue Song; Wang, Meng Qi; Wang, Lei Lei; Wu, Xue; Chen, Xi; Cao, Hong; Yang, Zhi Min

doi:10.1016/j.ecoenv.2019.03.040

Cited by 95 publications

(50 citation statements)

References 43 publications

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“…In our study, it was observed that inoculation of C. cupreum caused a down-regulation of genes involved in the detoxification of metals including, metallothionein-like protein 1 that acts by sequestering metals through thiol groups of their cysteine residues, whose distribution influences the capacity of union and sequestration of metals to maintain homeostasis [31]. The metallochaperone heavy metal-associated isoprenylated plant protein 28 ( HIPP28 ), that acts against the excess of metals, binding them through their cysteine residues and transporting them to intracellular compartments, helping with the detoxification of metals and maintaining homeostasis [32], and peroxidase 10 precursor acts decomposing hydrogen peroxide generated in response to oxidative stress in addition to participating in the oxidation of reducing toxins, lignin biosynthesis, suberization and auxin metabolism, were also down-regulated [33].…”

Section: Discussionmentioning

confidence: 99%

The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

et al. 2019

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The endophytic strain Chaetomium cupreum isolated from metal-contaminated soil was inoculated in Eucalyptus globulus roots to identify genes involved in metal stress response and plant growth promotion. We analyzed the transcriptome of E. globulus roots inoculated with C. cupreum. De novo sequencing, assembly, and analysis were performed to identify molecular mechanisms involved in metal stress tolerance and plant growth promotion. A total of 393,371,743 paired-end reads were assembled into 135,155 putative transcripts. It was found that 663 genes significantly changed their expression in the presence of treatment, of which 369 were up-regulated and 294 were down-regulated. We found differentially expressed genes (DEGs) encoding metal transporters, transcription factors, stress and defense response proteins, as well as DEGs involved in auxin biosynthesis and metabolism. Our results showed that the inoculation of C. cupreum enhanced tolerance to metals and growth promotion on E. globulus. This study provides new information to understand molecular mechanisms involved in plant–microbe interactions under metals stress.

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Section: Discussionmentioning

confidence: 99%

The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

et al. 2019

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“…The co-expression of OsLCT1, OsHMA2 and OsZIP3 transporters increases the uptake, translocation and potential oxidative stress of Cd and Zn in Oryza sativa [187]. Khan et al [89] identified two novel rice genes HPP (heavy metal-associated plant protein) and HIPP (heavy metal-associated isoprenylated plant protein) tolerant to Cu, Zn, Cd and Mn. In transgenic tobacco, Gouiaa and Khoudi [62] demonstrated that expression of vacuolar proton pump (V-PPase) with a Na/ proton antiporter (NHX1 transporter) enhances Cu tolerance and accumulation in transgenic tobacco.…”

Section: Biotechnological Processesmentioning

confidence: 99%

Phytoremediation: a sustainable environmental technology for heavy metals decontamination

Nedjimi

2021

SN Appl. Sci.

258

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Toxic metal contamination of soil is a major environmental hazard. Chemical methods for heavy metal's (HMs) decontamination such as heat treatment, electroremediation, soil replacement, precipitation and chemical leaching are generally very costly and not be applicable to agricultural lands. However, many strategies are being used to restore polluted environments. Among these, phytoremediation is a promising method based on the use of hyper-accumulator plant species that can tolerate high amounts of toxic HMs present in the environment/soil. Such a strategy uses green plants to remove, degrade, or detoxify toxic metals. Five types of phytoremediation technologies have often been employed for soil decontamination: phytostabilization, phytodegradation, rhizofiltration, phytoextraction and phytovolatilization. Traditional phytoremediation method presents some limitations regarding their applications at large scale, so the application of genetic engineering approaches such as transgenic transformation, nanoparticles addition and phytoremediation assisted with phytohormones, plant growth-promoting bacteria and AMF inoculation has been applied to ameliorate the efficacy of plants as candidates for HMs decontamination. In this review, aspects of HMs toxicity and their depollution procedures with focus on phytoremediation are discussed. Last, some recent innovative technologies for improving phytoremediation are highlighted.

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“…All HMPs were divided into six clades according to the characteristics of the HMA domain (Figure 6). In a previous study, HPP and HIPP were divided into five groups and the proteins in the ATCCS group were also included (Khan et al, 2019). The latter were named in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Rice (Oryza sativa L.) and Arabidopsis thaliana L. are research models for monocotyledonous and dicotyledonous plants, respectively. In earlier research, different heavy metal gene families in various species were studied or classified separately (Li et al, 2015;Fang et al, 2016;Zhiguo et al, 2018;Khan et al, 2019). Here, we identified all heavy metal-associated proteins in rice and Arabidopsis, including HPP, HIPP, ATX, CCH, CCS, and P1B-ATPase HMPs, by repeated HMM searches in silico.…”

Section: Introductionmentioning

confidence: 99%

Heavy Metal Stress-Associated Proteins in Rice and Arabidopsis: Genome-Wide Identification, Phylogenetics, Duplication, and Expression Profiles Analysis

et al. 2020

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Heavy metal exposure is a serious environmental stress in plants. However, plants have evolved several strategies to improve their heavy metal tolerance. Heavy metal-associated proteins (HMPs) participate in heavy metal detoxification. Here, we identified 46 and 55 HMPs in rice and Arabidopsis, respectively, and named them OsHMP 1-46 and AtHMP 1-55 according to their chromosomal locations. The HMPs from both plants were divided into six clades based on the characteristics of their heavy metal-associated domains (HMA). The HMP gene structures and motifs varied greatly among the different classifications. The HMPs had high collinearity and were segmentally duplicated. A cis-element analysis revealed that the HMPs may be regulated by different transcription factors. An expression profile analysis disclosed that only eight OsHMPs were constitutive in rice tissues. Of these, the expression of OsHMP37 was far higher than that of the other seven genes while OsHMP28 was expressed exclusively in the roots. For Arabidopsis, nine AtHMPs presented with very high transcript levels in all organs. Most of the selected OsHMPs were differentially expressed in various tissues under different heavy metal stresses. Only OsHMP09, OsHMP18, and OsHMP22 showed higher expression levels in all tissues under different heavy metal stresses. In contrast, most of the selected AtHMPs had nearly constant expression levels in different tissues under various heavy metal stresses. The AtHMP20, AtHMP23, AtHMP25, AtHMP31, AtHMP35, AtHMP46 expression levels under different heavy metal stresses were higher in the leaves and roots. The foregoing discoveries elucidated HMP evolution in monocotyledonous and dicotyledonous plants and may helpful functionally characterize HMPs in the future.

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Identification of novel rice (Oryza sativa) HPP and HIPP genes tolerant to heavy metal toxicity

Cited by 95 publications

References 43 publications

The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

Phytoremediation: a sustainable environmental technology for heavy metals decontamination

Heavy Metal Stress-Associated Proteins in Rice and Arabidopsis: Genome-Wide Identification, Phylogenetics, Duplication, and Expression Profiles Analysis

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