Identification of ochratoxin A in Chinese spices using HPLC fluorescent detectors with immunoaffinity column cleanup

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

“…When immobilized antibodies are used to isolate a target prior to its measurement or analysis by a second method, this approach is often referred to as immunoextraction [7,8]. This may be carried out by using columns that are prepared in-house, or by using one of the growing number of columns that are available commercially for this type of work [48][49][50][51][52]. In many cases, the column that is used for immunoextraction can be placed on-line with the second analysis method, as has been used with various forms of HPLC, MS and…”

“…Immunoextraction columns have been used off-line with ultra-high performance LC (UHPLC) and MS/MS for the determination of aflatoxin M 1 in ice cream [49], and immunoaffinity columns were utilized off-line with LC-MS/MS for the analysis of multiple mycotoxins in food [50,51]. In addition, immunoextraction has been coupled both on-line and off-line with HPLC and fluorescence detection for the measurement of aflatoxins and mycotoxins in food [48,52,54], and used off-line with HPLC for the measurement of phenylethanolamine in animal feed and meat or liver samples [55]. Immobilized antibody fragments have been used to analyze the enantiomers of diarylalkyltriazole [56], and an immunoaffinity column has been used to separate ginsenoside epimers [57].…”

Multiclass mycotoxins in lotus seeds analysed by an isotope-labelled internal standard-based UPLC-MS/MS

“…Commonly reported methods have got wide application for mycotoxins determination in different matrices. However, most of these methods are mainly focused on a single mycotoxin or multiple mycotoxins belonging to the same group or class . Usually, the herbs and/or foods matrices may be contaminated by multiple mycotoxins of different groups of classes .…”

“…However, most of these methods are mainly focused on a single mycotoxin or multiple mycotoxins belonging to the same group or class. [25,26] Usually, the herbs and/or foods matrices may be contaminated by multiple mycotoxins of different groups of classes. [27,28] So, reliable methods for simultaneous determination of multiclass mycotoxins are of great interest.…”

A reliable screening of mycotoxins and pesticide residues in paprika using ultra-high performance liquid chromatography coupled to high resolution Orbitrap mass spectrometry

“…Elevated concentrations of OTA and AFB 1 have been found in more than 40% of paprika samples produced in the EU countries (Zhao, Yuan, Zhang, & Yue, 2014). Within the field of spice contamination analysis, multi-mycotoxin determination based on the triple quadrupole tandem mass spectrometry (QqQ-MS/MS) technique (Boonzaaijer, Van Osenbruggen, Kleinnijenhuis, & Van Dongen, 2008;Desmarchelier et al, 2014) is of increasing importance.…”