Multiclass mycotoxins in lotus seeds analysed by an isotope-labelled internal standard-based UPLC-MS/MS

Huang, Pinxuan; Kong, Weijun; Wang, Sha; Lu, Jinghua; Yang, Meihua

doi:10.1111/jphp.12974

“…A similar situation was confirmed for matrix-match calibration [45,49,70], and internal standard (IS) addition [48,66,77] where a clean-up step was not necessary to eliminate matrix effects and run accurate determinations. Yet, the use of specific IS and a validated method for a single matrix, could reduce the scope of the determination, and increase its final cost.…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 66%

“…Fluorescence detectors are restricted for the individual quantification of FB1 [38,46,47], the sum of FB1, FB2, FB3 [41] or the separate determination of up to three group B fumonisins [42,55]. On the other hand, one of the main advantages of mass spectrometry detectors is the possibility of performing multiplex analysis, not only for different mycotoxins [48,49,50,53,54,57,58,59,60,61,64,65,66,67,71,72,73,74,77,79,80,187], but also when combined with varied metabolites. Growth regulators, antibiotics, pesticides [51,70,75], and other fungal metabolites [63,68], were simultaneously identified in analysis capable of assessing up to 74 and 90 compounds [62,78].…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida¹,

Gong²,

Goycoolea³

2020

Preprint

1

0

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Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

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“…Zeng et al employed UHPLC-triple quadrupole-MS/MS to determine 20 major constituents including salvianolic acids, tanshinones, flavonoids and triterpenes in different parts of Salvia miltiorrhiza [ 52 ]. Furthermore, other than active components determination, LC–MS has also been applied in analysis of toxins such as pesticide residue [ 53 , 54 ] and mycotoxins [ 55 – 57 ]. In ChP, LC–MS has been used for the test of adonifoline, a toxic alkaloid in Senecionis Scandentis Herba.…”

Section: Advanced Analytical Techniques For Quality Standard Of Tcmmentioning

confidence: 99%

“…[52]. Furthermore, other than active components determination, LC-MS has also been applied in analysis of toxins such as pesticide residue [53,54] and mycotoxins [55][56][57]. In ChP, LC-MS has been used for the test of adonifoline, a toxic alkaloid in Senecionis Scandentis Herba.…”

Section: Uhplc and Lc-msmentioning

confidence: 99%

Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances

Leong

¹

,

Xue

²

,

Wang

³

et al. 2020

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Traditional Chinese medicine (TCM) are becoming more and more popular all over the world. However, quality issues of TCM may lead to medical incidents in practice and therefore quality control is essential to TCM. In this review, the state of TCM in European Pharmacopoeia are compared with that in Chinese Pharmacopoeia, and herbal drugs that are not considered as TCM and not elaborated by TCM working party at European Directorate for the Quality of Medicines & Health Care (EDQM) but present in both European Pharmacopoeia and Chinese Pharmacopoeias are also discussed. Different aspects in quality control of TCM including origins, identification, tests and assays, as well as sample preparation, marker selection and TCM processing are covered to address the importance of establishing comprehensive quality standard of TCM. Furthermore, advanced analytical techniques for quality control and standard establishment of TCM are also reviewed. Electronic supplementary material The online version of this article (10.1186/s13020-020-00357-3) contains supplementary material, which is available to authorized users.

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“…Fluorescence detectors are restricted for the individual quantification of FB1 [38,46,47], the sum of FB1, FB2, FB3 [41] or the separate determination of up to three group B fumonisins [42,55]. On the other hand, one of the main advantages of mass spectrometry detectors is the possibility of performing multiplex analysis, not only for different mycotoxins [48,49,50,53,54,57,58,59,60,61,64,65,66,67,71,72,73,74,77,79,80,187], but also when combined with varied metabolites. Growth regulators, antibiotics, pesticides [51,70,75], and other fungal metabolites [63,68], were simultaneously identified in analysis capable of assessing up to 74 and 90 compounds [62,78].…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida

¹

,

Gong²,

Goycoolea³

2020

Preprint

1

0

View full text Add to dashboard Cite

Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

show abstract

Multiclass mycotoxins in lotus seeds analysed by an isotope-labelled internal standard-based UPLC-MS/MS

Cited by 18 publications

References 32 publications

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

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