Identification of Odorant Binding Proteins and Chemosensory Proteins in Antennal Transcriptomes of the Jumping Bristletail<i>Lepismachilis y-signata</i>and the Firebrat<i>Thermobia domestica:</i>Evidence for an Independent OBP–OR Origin

Missbach, Christine; Vogel, Heiko; Hansson, Bill S.; Große-Wilde, Ewald

doi:10.1093/chemse/bjv050

Cited by 32 publications

(32 citation statements)

References 62 publications

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“…The greater numbers found in Collembola, with respect to the other two classes simply reflects the greater attention given to this group to date. Similarly, transcriptome studies of Lepysmachilis (order Archaeognatha) and Thermobia (order Zygentoma) identified 40 and 32 OBP genes, and three and six CSP genes, respectively (Missbach et al, 2015). Such relatively large numbers of soluble olfactory proteins indicate that extensive duplication and differentiation must have taken place in basal Hexapoda, especially for OBPs, while CSPs expansion may be limited to Collembola.…”

Section: Figmentioning

confidence: 96%

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Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

et al. 2017

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Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are regarded as carriers of pheromones and odorants in insect chemoreception. These proteins are typically located in antennae, mouth organs and other chemosensory structures; however, members of both classes of proteins have been detected recently in other parts of the body and various functions have been proposed. The best studied of these non-sensory tasks is performed in pheromone glands, where OBPs and CSPs solubilise hydrophobic semiochemicals and assist their controlled release into the environment. In some cases the same proteins are expressed in antennae and pheromone glands, thus performing a dual role in receiving and broadcasting the same chemical message. Several reports have described OBPs and CSPs in reproductive organs. Some of these proteins are male specific and are transferred to females during mating. They likely carry semiochemicals with different proposed roles, from inhibiting other males from approaching mated females, to marking fertilized eggs, but further experimental evidence is still needed. Before being discovered in insects, the presence of binding proteins in pheromone glands and reproductive organs was widely reported in mammals, where vertebrate OBPs, structurally different from OBPs of insects and belonging to the lipocalin superfamily, are abundant in rodent urine, pig saliva and vaginal discharge of the hamster, as well as in the seminal fluid of rabbits. In at least four cases CSPs have been reported to promote development and regeneration: in embryo maturation in the honeybee, limb regeneration in the cockroach, ecdysis in larvae of fire ants and in promoting phase shift in locusts. Both OBPs and CSPs are also important in nutrition as solubilisers of lipids and other essential components of the diet. Particularly interesting is the affinity for carotenoids of CSPs abundantly secreted in the proboscis of moths and butterflies and the occurrence of the same (or very similar CSPs) in the eyes of the same insects. A role as a carrier of visual pigments for these proteins in insects parallels that of retinol-binding protein in vertebrates, a lipocalin structurally related to OBPs of vertebrates. Other functions of OBPs and CSPs include anti-inflammatory action in haematophagous insects, resistance to insecticides and eggshell formation. Such multiplicity of roles and the high success of both classes of proteins in being adapted to different situations is likely related to their stable scaffolding determining excellent stability to temperature, proteolysis and denaturing agents. The wide versatility of both OBPs and CSPs in nature has suggested several different uses for these proteins in biotechnological applications, from biosensors for odours to scavengers for pollutants and controlled releasers of chemicals in the environment.

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Section: Figmentioning

confidence: 96%

“…During evolution, both OBPs and CSPs seem to have undergone much duplication and differentiation in Hexapoda similarly to ORs that evolved in insects after the emergence of Archaeognatha and Zygentoma (Missbach et al, 2014).…”

Section: (3) Obps and Csps Across Evolutionmentioning

confidence: 99%

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

et al. 2017

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“…By using similar methods as for OBP identification, many CSPs have been discovered in distinct insects (Guo et al, 2011; Iovinella et al, 2013; Jacquin-Joly et al, 2001; Liu et al, 2010; Missbach et al, 2015; Picimbon et al, 2001; Wanner et al, 2004). Unlike OBPs, CSPs are smaller and more conserved in distinct insects, which only have four conserved cysteines that form two interlocking disulphide bridges (Bohbot et al, 1998; Lartigue et al, 2002; Maleszka & Stange, 1997; Pelosi, Calvello & Ban, 2005; Zhang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

Zhang

Zhu

et al. 2017

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The olfaction system of insects plays an important role in mediating various physiological behaviors, including locating hosts, avoiding predators, and recognizing mates and oviposition sites. Therefore, some key genes in the system present valuable opportunities as targets for developing novel green pesticides. Athetis lepigone, a noctuid moth can feed on more than 30 different host plants making it a serious polyphagous pest worldwide, and it has become one of the major maize pests in northern China since 2011. However, there are no reports on effective and environmentally friendly pesticides for the control of this pest. In this study, we identified 28 genes encoding putative odorant binding proteins (OBPs) and 20 chemosensory protein (CSPs) genes based on our previous A. lepigone transcriptomic data. A tissue expression investigation and phylogenetic analysis were conducted in an effort to postulate the functions of these genes. Our results show that nearly half (46.4%) of the AlOBPs exhibited antennae-biased expression while many of the AlCSPs were highly abundant in non-antennal tissues. These results will aid in exploring the chemosensory mechanisms of A. lepigone and developing environmentally friendly pesticides against this pest in the future.

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“…In the case of GRs and ORs, sequences from the waterflea Daphnia pulex were also included as an outgroup (Peñalva-Arana et al, 2009). For the CSPs and OBPs we included sequences from the Jumping Bristletail Lepismachilis y-signata and the Firebrat Thermobia domestica (Missbach et al, 2015). In the case of IRs (and Glu-Rs) sequences from Drosophila melanogaster have been included (Rytz et al, 2013).…”

Section: Alignments and Phylogenetic Treesmentioning

confidence: 99%

“…CSPs are likely to perform similar roles in chemical communication of insects as OBPs, but unlike these are also expressed in non-chemosensory tissues, and for this reason have been hypothesized to serve additional, as yet undiscovered, functions (Pelosi et al, 2005). Recent evidence suggests that OBPs are an adaptation to the detection of hydrophobic volatiles that became available as olfactory cues in the course of insect terrestrialization (Missbach et al, 2015); however, results in Drosophila suggest a different function for some OBPs (Larter et al, 2016). Structurally, insect OBPs and CSPs generally contain α-helical domains, but folded in two different patterns (Sandler et al, 2000;Lartigue et al, 2002;Tegoni et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Comparing the Expression of Olfaction-Related Genes in Gypsy Moth (Lymantria dispar) Adult Females and Larvae from One Flightless and Two Flight-Capable Populations

et al. 2017

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In insects, flight and sophisticated olfactory systems go hand in hand and are essential to survival and evolutionary success. Females of many Lepidopteran species have secondarily lost their flight ability, which may lead to changes in the olfactory capabilities of both larval and adult stages. The gypsy moth, Lymantria dispar, an important forest pest worldwide, is currently undergoing a diversification process with three recognized subspecies: the Asian gypsy moth (AGM), Lymantria dispar asiatica; the Japanese gypsy moth (JGM), Lymantria dispar japonica; and the European gypsy moth (EGM), Lymantria dispar dispar. Females of EGM populations from North America have lost their flight capacity whereas the JGM and AGM females are flight capable, making this an ideal system to investigate the relationship between flight and olfaction. We used next-generation sequencing to obtain female antennal and larval head capsule transcriptomes in order to (i) investigate the differences in expression of olfaction-related genes among populations; (ii) identify the most similar protein sequences reported for other organisms through a BLAST search, and (iii) establish the phylogenetic relationships of these sequences with respect to other insect species. Using this approach, we identified 115 putative chemosensory genes belonging to five families of olfaction-related genes. A principal component analysis (PCA) revealed that the gene-expression patterns of female antennal transcriptomes from different subspecies were more similar to one another than to the larval head capsules of their respective subspecies supporting strong chemosensory differences between the two developmental stages. An analysis of the shared and exclusively expressed genes for three populations shows no evidence that loss of flight affects the number or type of genes being expressed. These results indicate either (a) that loss of flight does not impact the olfactory gene repertoire or (b) that the secondary loss of flight in American EGM populations may be too recent to have caused major changes in the genes being expressed. However, we found higher expression values for Clavijo McCormick et al.Gypsy Moth Olfactory Gene Expression most olfaction-related genes in EGM females, suggesting that differences in transcription rates could be an adaptation of flightless females to their chemical environment. Differences in olfactory genes and their expression in the larvae appear to be unrelated to the flight ability of adult females and are likely adaptations to different ecological pressures.

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Identification of Odorant Binding Proteins and Chemosensory Proteins in Antennal Transcriptomes of the Jumping BristletailLepismachilis y-signataand the FirebratThermobia domestica:Evidence for an Independent OBP–OR Origin

Cited by 32 publications

References 62 publications

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

Comparing the Expression of Olfaction-Related Genes in Gypsy Moth (Lymantria dispar) Adult Females and Larvae from One Flightless and Two Flight-Capable Populations

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