Identification of potential biomarkers for clear cell renal cell carcinoma based on microRNA-mRNA pathway relationships

Hao, Junfeng; Ren, Kaiming; Bai, Jiuxu; Wang, Shou-Nan; Lei, Lei; Cao, Ning; Li, Xu

doi:10.4103/0973-1482.145856

Cited by 21 publications

(12 citation statements)

References 43 publications

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“…Though numerous studies have been focused on miRNAs, the functions of most miRNAs remain poorly understood. MiRNA-320d (miR-320d) is a member of miRNA-320 family, and acts as a tumor suppressor but has a very low expression level in several human malignant tumors [14][15][16]. However, very few researches have focused on this miRNA effects on gastric cardia adenocarcinoma (GCA).…”

Section: Discussionmentioning

confidence: 99%

“…Although recent studies have reported the functions of miR-320d in several cancer types, such as colorectal cancer [15], renal cell carcinoma [16], glioma [23], et al, less studies are focusing on the potential targeted genes of miR-320d. As the classical function of microRNA is silencing targeted genes by completely or incompletely pairing to the 3′-UTR of the specific genes, we screened the miR-320d targeted genes through the TargetScan website and other microRNA databases, and eventually found that FoxM1 contains two mRNA 3′-UTR binding sites for miR-320d.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, more than 1000 miRNAs are reported in encoding in the human genome. Among these revealed miRNAs, microRNA-320d (miR-320d), a member of miR-320 family, has been reported to be involved in different malignant tumors, including large B cell lymphoma [14], colon cancer [15], renal cell carcinoma [16]. However, studies focusing on the role and mechanism of miR-320d in the progression and development of GCA are still lacking.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

et al. 2020

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Recent evidences demonstrate that dysregulated expression of microRNA-320d (miR-320d) has been associated with several cancer development and progression. However the effect of miR-320d on gastric cardiac adenocarcinoma (GCA) and the association of miR-320d with its potential gene target FoxM1 remain unclear. Here, we evaluated expression profile of miR-320d and FoxM1 in 60 human GCA tissues and GCA cell lines (OE-19 and SK-GT2). Immunohistochemistry, qualitative PCR and western-blotting were performed in GCA tissues to detect the expression level of miR-320d and FoxM1. CCK-8, transwell, wound-healing assays, and in vivo experiments were conducted using GCA cells that treated with miR-320d mimics or inhibitors to evaluate the biological functions of miR-320d. Luciferase reporter assay was conducted to confirm possible binding sites of FoxM1 for miR-320d. Compared with paired noncancerous tissues, it showed that miR-320d expression was significantly decreased in GCA specimens (P < 0.0001), while FoxM1 was significantly upregulated in GCA tissues (P < 0.0001). Modulating miR-320d function by transfection of miR-320 mimics or inhibitor led to inhibition or promotion of GCA cell proliferation and invasion, thus regulating tumor progression in GCA-tumor bearing mice. The mechanism analysis of miR-320d/FoxM1 showed that FoxM1 has two miR-320d binding sites in its 3′-untranslated region (3′-UTR), that contributes to regulation of the cell biological behaviors. Taken together, our data suggested that miR-320d acts as a tumor suppressor in GCA by directly targeting FoxM1 and thus potentially serves as a biomarker for anti-GCA therapy in GCA patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

et al. 2020

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“…And the present review highlighted the role of glutathione and related cytoprotective effects in the susceptibility to carcinogenesis and in the sensitivity of tumors to the cytotoxic effects of anticancer agents [ 40 ]. Recently, Hao et al discovered that three significant pathways related to ccRCC, namely, arginine and proline metabolism, aldosterone-regulated sodium reabsorption, and oxidative phosphorylation, were observed [ 41 ]. Arginine/proline metabolism is a significant pathway in ccRCC that had been discovered by Perroud et al previously [ 42 ], and the results were in accordance with our analysis.…”

Section: Discussionmentioning

confidence: 99%

Temporal Identification of Dysregulated Genes and Pathways in Clear Cell Renal Cell Carcinoma Based on Systematic Tracking of Disrupted Modules

Wang

Sun

Liu

et al. 2015

Computational and Mathematical Methods in Medicine

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Objective. The objective of this work is to identify dysregulated genes and pathways of ccRCC temporally according to systematic tracking of the dysregulated modules of reweighted Protein-Protein Interaction (PPI) networks. Methods. Firstly, normal and ccRCC PPI network were inferred and reweighted based on Pearson correlation coefficient (PCC). Then, we identified altered modules using maximum weight bipartite matching and ranked them in nonincreasing order. Finally, gene compositions of altered modules were analyzed, and pathways enrichment analyses of genes in altered modules were carried out based on Expression Analysis Systematic Explored (EASE) test. Results. We obtained 136, 576, 693, and 531 disrupted modules of ccRCC stages I, II, III, and IV, respectively. Gene composition analyses of altered modules revealed that there were 56 common genes (such as MAPK1, CCNA2, and GSTM3) existing in the four stages. Besides pathway enrichment analysis identified 5 common pathways (glutathione metabolism, cell cycle, alanine, aspartate, and glutamate metabolism, arginine and proline metabolism, and metabolism of xenobiotics by cytochrome P450) across stages I, II, III, and IV. Conclusions. We successfully identified dysregulated genes and pathways of ccRCC in different stages, and these might be potential biological markers and processes for treatment and etiology mechanism in ccRCC.

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“…miR-425-5p is located on human chromosome 3 and is aberrantly expressed in various types of cancer, including gastric ( 9 ), cervical ( 10 ) and hepatocellular carcinoma ( 11 ). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis indicated an overexpression of miR-425-5p in RCC tissues, thus suggesting its potential use as a biomarker for the diagnosis of RCC ( 12 , 13 ). However, the molecular mechanisms underlying the effects miR-425-5p in RCC remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Oncogenic miR‑425‑5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Quan

Pan

et al. 2018

Oncol Lett

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An increasing number of studies have demonstrated the function of microRNAs (miRNAs) in the initiation and development of various types of cancer. Among them, miR-425-5p is proven to serve an important function in several types of cancer, including gastric, cervical cancer, and hepatocellular carcinoma. However, the function of miR-425-5p in renal cell carcinoma (RCC) remains unclear. In the present study, it was demonstrated that the expression level of miR-425-5p was upregulated in RCC tissues and cell lines compared with normal tissues and cell lines (P<0.05). Additionally, Cell Counting kit-8 and MTT assays were employed to assess cell viability and proliferation, whereas wound healing and Transwell assays were employed to examine migration and invasion. The results demonstrated that upregulation of miR-425-5p promoted cell viability and the invasion and migration of ACHN and 786O cells (P<0.05). Flow cytometric analysis confirmed that upregulation of miR-425-5p inhibited apoptosis of ACHN and 786O cells (P<0.05). Downregulation of miR-425-5p inhibited the viability and invasion and migration of ACHN and 786O cells (P<0.05). In the present study, upregulation of miR-425-5p inhibited apoptosis of ACHN and 786O cells whereas no differences in early apoptotic rate were observed between the inhibitor and inhibitor NC groups for 786O and ACHN cells. These results indicate that miR-425-5p may act as an oncogene in RCC.

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Identification of potential biomarkers for clear cell renal cell carcinoma based on microRNA-mRNA pathway relationships

Abstract: This integrative network approach revealed important miRNAs in the ccRCC that can identify specific disease biomarkers, which can be used as targets for cancer treatment.

Cited by 21 publications

References 43 publications

MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

Temporal Identification of Dysregulated Genes and Pathways in Clear Cell Renal Cell Carcinoma Based on Systematic Tracking of Disrupted Modules

Oncogenic miR‑425‑5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

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