Identification of Potential Probiotics in the Midgut of Mulberry Silkworm, Bombyx mori Through Metagenomic Approach

Thirupathaiah, Yeruva; Sivaprasad, V.; Sumathy, R.; Lingaiah, Kusuma

doi:10.1007/s12602-019-09580-3

Cited by 21 publications

(25 citation statements)

References 22 publications

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“…Recently, Yeruva et al [ 20 ] used 16S sequencing to investigate the presence of potential probiotics in B. mori and found that one of the dominating genera was Lactobacillus . We hypothesized that feeding Lactobacillus bacteria could reduce the number of dead silkworm larvae challenged by N. bombycis and found significant positive effects.…”

Section: Introductionmentioning

confidence: 99%

Supplementation of Lactobacillus casei reduces the mortality of Bombyx mori larvae challenged by Nosema bombycis

Suraporn

Terenius

2021

BMC Res Notes

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Objective Pebrine, caused by the microsporidium Nosema bombycis, is one of the severe diseases in Thai polyvoltine strains of the silkworm Bombyx mori. Studies showing the presence of Lactobacillus species in the silkworm gut, where the Nosema parasites enter, suggests that these bacteria may have a protective effect. The aim of this study was to investigate the effect of supplementation of Lactobacillus casei on the survival ratio of silkworm larvae challenged with N. bombycis. Results A group of silkworm larvae of the commercial Thai polyvoltine hybrid strain DokBua was supplemented with L. casei on the second day of the 2nd, 3rd, 4th, and 5th instar. When a control group of silkworm larvae were challenged with N. bombycis on the second day of the 4th instar, the survival rate was 68%, but it was 91% for larvae supplemented with L. casei. For those larvae that survived the treatments until pupation, we determined the growth characters larval weight, cocooning ratio, and pupation ratio, and the economic characters cocoon weight and cocoon shell weight. When infected with N. Bombycis, growth characters were significantly higher in larvae also receiving L. casei.

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Section: Introductionmentioning

confidence: 99%

Supplementation of Lactobacillus casei reduces the mortality of Bombyx mori larvae challenged by Nosema bombycis

Suraporn

Terenius

2021

BMC Res Notes

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“…Enterococcus and Lactobacillus were two main residents found in the midgut of mulberry silkworm, Bombyx mori. In addition, their relative abundance was varied depending on different kinds of silkworms which contributed to their physiological activities [29]. In the previous studies, E. mundtii QU25 was reported for its potential use for LA production from xylose.…”

Section: Molecular Identification Of Labmentioning

confidence: 99%

Improvement of Enantiomeric l-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of Enterococcus mundtii WX1 and Lactobacillus rhamnosus SCJ9

et al. 2021

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Among 39 pentose-utilizing lactic acid bacteria (LAB) selected from acid-forming bacteria from the midgut of Eri silkworm, the isolate WX1 was selected with the highest capability to produce optically pure l-lactic acid (l-LA) from glucose, xylose and arabinose with furfural-tolerant properties. The isolate WX1 was identified as Enterococcus mundtii based on 16S rDNA sequence analysis. The conversion yields of l-LA from glucose and xylose by E. mundtii WX1 were 0.97 and 0.68 g/g substrate, respectively. Furthermore, l-LA production by E. mundtii WX1 in various glucose-xylose mixtures indicated glucose repression effect on xylose consumption. The coculture of E. mundtii WX1 and Lactobacillus rhamnosus SCJ9, a homofermentative LAB capable of producing l-LA from glucose clearly showed an improvement of l-LA production from 30 g/L total glucose-xylose (6:4). The results from Plackett–Burman design (PBD) indicated that Tween 80, MnSO4 and yeast extract (YE) were three medium components that significantly influenced (p < 0.05) l-LA production using the coculture strategy in the presence of 2 g/L furfural. Optimal concentrations of these variables revealed by central composite design (CCD) and response surface methodology (RSM) were 20.61 g/L YE, 1.44 g/L Tween 80 and 1.27 g/L MnSO4. Based on the optimized medium with 30 g/L total glucose-xylose (6:4), the maximum experimental l-LA value of 23.59 g/L reflecting 0.76 g/g substrate were achieved from 48 h fermentation at 37 °C. l-LA produced by coculture cultivated under standard MRS medium and new optimized conditions were 1.28 and 1.53 times higher than that obtained from single culture by E. mundtii WX1, respectively. This study provides the foundations for practical applications of coculture in bioconversion of lignocellulose particularly glucose-xylose-rich corn stover to l-LA.

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“…Previous studies have proven that B. subtilis can be used as feed additives in animals (Guo et al, 2017;Ma et al, 2018;Zaineldin et al, 2018) and can have beneficial effects on humans (Lefevre et al, 2017). In addition, Bacillus is a potential probiotic that can improve the economic traits of silkworm (Yeruva et al, 2020). Moreover, orally administered B. subtilis improves silkworm growth and development by producing a wide variety of digestive enzymes (Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

Zheng

Zhu

et al. 2022

Environmental Microbiology

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The symbiotic association between bacterial symbionts and insect hosts is a complicated process that is not completely understood. Herein, we used a silkworm model to study the association between symbiotic Bacillus and lepidopteran insect by investigating the changes in intestinal microbiota and hemolymph circulating metabolites of silkworm after symbiotic Bacillus subtilis treatment. Results showed that B. subtilis can generate a variety of primary and secondary metabolites, such as B vitamins and antimicrobial compounds, to provide micronutrients and enhance the pathogen resistance of their insect host. Shifts in the relative abundance of Enterococcus, Brevibacterium, Buttiauxella, Pseudomonas, Brevundimonas and Limnobacter had significant correlations with the concentrations of differential metabolites (e.g. phospholipids and certain amino acids) in insect hemolymph. The antimicrobial compounds secreted by B. subtilis were the primary driving force for the reconstruction of intestinal microbiota. Meanwhile, the altered levels of circulating metabolites in multiple metabolic pathways were potential adaptive mechanism of insect hosts in response to the shifts of intestinal microbiota. Our findings provided concrete evidence that bacterial intestinal symbiont can alter the physiological state of insects and highlighted the importance of the compositional alterations of intestinal microbiota as a source of variation in circulating metabolites of insect hosts.

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Identification of Potential Probiotics in the Midgut of Mulberry Silkworm, Bombyx mori Through Metagenomic Approach

Cited by 21 publications

References 22 publications

Supplementation of Lactobacillus casei reduces the mortality of Bombyx mori larvae challenged by Nosema bombycis

Supplementation of Lactobacillus casei reduces the mortality of Bombyx mori larvae challenged by Nosema bombycis

Improvement of Enantiomeric l-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of Enterococcus mundtii WX1 and Lactobacillus rhamnosus SCJ9

Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

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