Identification of quantitative trait loci associated with powdery mildew resistance in mungbean using ISSR and ISSR-RGA markers

Poolsawat, O.; Kativat, Chitpan; Arsakit, K.; Tantasawat, Piyada Alisha

doi:10.1007/s11032-017-0753-2

Cited by 24 publications

(30 citation statements)

References 31 publications

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“…The size of the fragments obtained with the RGA primers being considered in regard to two classifications (adequate and reasonable) ranged from 200 to 2.000 bp. These results corroborate Poolsawat, Kativat, Arsakit and Tantasawat (2017), who reported bands between 100 and 2.072 bp when associated with ISSR-RGA primers for Vigna radiata L. Wilczek. The 'A' pairs produced 68 markers in the 20 accessions studied, averaging 5.7 markers per primer pair.…”

Section: Resultssupporting

confidence: 91%

Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

et al. 2021

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The characterization and selection of molecular markers are important for genetic pre-breeding programs since they make it possible to choose the most appropriate markers to be used in future research. Therefore, enabling the generation of subsidies for genetic-molecular studies in algabora (Prosopis juliflora (Sw.) DC). The amplification profile was characterized. It was generated from 17 pairs of RGA primers (Resistance Gene Analogs) in 20 samples of genomic DNA of P. juliflora extracted from specimens collected in the city of Itapetinga, Bahia. The amplifications were performed according to previously published laboratory routines and the amplification profiles analyzed from the photodocumentation of the electrophoresis results in 2% agarose gels. Based on the amplification profiles the primer pairs were classified as: Suitable: amplifications in the whole samples and with easy visualization; Reasonable: amplification in parts of the samples and/or difficult to visualize or Inadequate: absence of visible amplification products. Descriptive analyzes associated with the number of generated markers, percentage of polymorphism, expected heterozygosity (He) and the content of polymorphic information (PIC) were also performed. In a nutshell, 12 out of the 17 pairs of RGA primers generated amplification products with easy visualization and only two of these 12 pairs of primers were monomorphic. The percentage of polymorphism varied from 60% to 100%, He and PIC presented an average of 0.21 (ranging from 0 to 0.38) and 0.17 (ranging from 0 to 0.29), respectively. The results confirm that the RGA primers present adequate characteristics for genetic studies in P. juliflora, making it possible to prioritize 12 pairs of primers, which are subject to genetic improvement studies.

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Section: Resultssupporting

confidence: 91%

Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

et al. 2021

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“…Several mungbean genotypes formed four clusters including cluster II, III, IV and V consisting of five, nine, five, and two genotypes, respectively. In cluster II, 'V4718', 'V4758', and 'V4785', originated in India whose genetics are related to disease resistance in Thailand (Poolsawat et al, 2017;Tantasawat et al, 2020;Tantasawat unpublished data) showed high resistance to CLS and PM (Table 4). However, the others failed in their resistance to both diseases, although some of them, i.e.…”

Section: Agronomic and Photosynthetic Character Analysismentioning

confidence: 99%

“…Clusters (number of genotypes) Individuals I ( 4) II ( 5) III ( 9) IV ( 5 Chankaew et al (2011);Poolsawat et al (2017) and Tantasawat (unpublished data), and found to be highly resistant to both diseases (Table 4) had genetic distance of 14.216. In subcluster IIA, 'SUT1', 'CN36', 'CN84-1', and 'EG-MD-6D' which had high yielding potential with regard to high yield/plant and large seed size and were grouped in the same subcluster IV of the dendrogram based on field performance (Figure 1A) exhibited high genetic relationships with only 10.729 genetic distance.…”

Section: Charactersmentioning

confidence: 99%

Genetic relationship of mungbean and blackgram genotypes based on agronomic and photosynthetic performance and SRAP markers

Chueakhunthod

Jinagool

Meecharoen

et al. 2020

Not Bot Horti Agrobo

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Genetic identification is at the base of parental selection in varietal development programs. Agronomic and photosynthetic characters and sequence-related amplified polymorphism (SRAP) markers were analyzed for two legume species which included 23 mungbean (Vigna radiata (L.) Wilczek) and four blackgram (Vigna mungo (L.) Hepper) genotypes. The results revealed that the seeds/pod, plant height, pods/plant, pod length, days to flowering, and all photosynthetic characters studied had a significant correlation with the yield/plant. Using UPGMA analysis with phenotypic data, five clusters and two individuals were identified. Twenty-five SRAP primer combinations generated 562 amplified bands, of which 507 were polymorphic (90.2%). The average numbers of scorable and polymorphic bands/primer pair were 22 and 20, respectively. Two major clusters coincided with two species having a 100% bootstrap value. Within the mungbean cluster, there were two subclusters containing 12 and 11 mungbean genotypes. Mantel’s test demonstrated that the polymorphisms given by SRAPs were associated with agronomic and photosynthetic variability (r = 0.734, p < 0.01). These results allow promising mungbean genotypes to be identified through genetic diversity and field performance which can be utilized as potential parents towards future breeding programs. Moreover, the factors which contribute most to yield/plant can be simultaneously used as selection criteria for yield improvement.

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“…Genome-wide association genetics is clearing the way for mapping agronomically important traits, but the low funds put into minor crops, such as mungbean, limits the population size for phenotyping and ultimately confines these efforts mainly to simple oligogenic or monogenic traits, and mapping bi-parental populations. Examples of traits recently mapped in mungbean are drought tolerance [23], seed starch content [24], high iron and zinc [25], salt tolerance [11] and resistance to powdery mildew [26], MYMD [27], and, as mentioned above, bruchid resistance [19] [20]. For most mapped loci, validation of their value for breeding is still lacking.…”

Section: Adaption Strategies and Breeding Progressmentioning

confidence: 99%

Beans with Benefits—The Role of Mungbean (<i>Vigna radiate</i>) in a Changing Environment

Pataczek¹,

Zahir²,

Ahmad³

et al. 2018

AJPS

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Dryland areas are experiencing low agricultural yields due to severe water shortages and salinity, leading to food scarcity. Mungbean (Vigna radiata) is gaining attention as a short-season crop that can tolerate dryland conditions, and fix atmospheric nitrogen, decreasing soil nutrient depletion. It is a source of high-quality protein for human consumption and can serve as a multipurpose crop, if harvest residues are used as fodder or green manure. However, little of this legume's potential has been explored. This review aims to underline the importance of mungbean as an agricultural crop by reviewing relevant literature on the potential contribution of mungbean to food security and a balanced diet as well as the effect of mungbean cultivation on farm income and gender equality. The challenges of climate change in plant production are discussed, and how progress in mungbean breeding and the application of improved cultivation techniques, such as regulated deficit irrigation, conservation agriculture, and inoculation with plant growth promoting rhizobacteria can overcome them.

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Identification of quantitative trait loci associated with powdery mildew resistance in mungbean using ISSR and ISSR-RGA markers

Cited by 24 publications

References 31 publications

Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

Genetic relationship of mungbean and blackgram genotypes based on agronomic and photosynthetic performance and SRAP markers

Beans with Benefits—The Role of Mungbean (<i>Vigna radiate</i>) in a Changing Environment

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Identification of quantitative trait loci associated with powdery mildew resistance in mungbean using ISSR and ISSR-RGA markers

Cited by 24 publications

References 31 publications

Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

Genetic relationship of mungbean and blackgram genotypes based on agronomic and photosynthetic performance and SRAP markers

Beans with Benefits—The Role of Mungbean (&lt;i&gt;Vigna radiate&lt;/i&gt;) in a Changing Environment

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Beans with Benefits—The Role of Mungbean (<i>Vigna radiate</i>) in a Changing Environment