Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine

Abstract

Oligonucleotide primers, designed to conserved regions of nucleotide binding site (NBS) motifs within previously cloned pathogen resistance genes, were used to amplify resistance gene analogs (RGAs) from grapevine. Twenty eight unique grapevine RGA sequences were identified and subdivided into 22 groups on the basis of nucleic acid sequence-identity of approximately 70% or greater. Representatives from each group were used in a bulked segregant analysis strategy to screen for restriction fragment length polymorphisms linked to the powdery mildew resistance locus, Run1, introgressed into Vitis vinifera L. from the wild grape species Muscadinia rotundifolia. Three RGA markers were found to be tightly linked to the Run1 locus. Of these markers, two (GLP1-12 and MHD145) cosegregated with the resistance phenotype in 167 progeny tested, whereas the third marker (MHD98) was mapped to a position 2.4 cM from the Run1 locus. The results demonstrate the usefulness of RGA sequences, when used in combination with bulked segregant analysis, to rapidly generate markers tightly linked to resistance loci in crop species.

Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)

“…This confirms that chilli being dicotyledonous, has both TIR and non-TIR NBS-LRR class genes. In our study the TIR: non-TIR type NBS-LRR RGAs ratio of 1:1 have been found, whereas high ratios were reported in other crops (Donald et al 2002). But in eggplant only non-TIR RGAs were isolated by Zhuang et al (2012).…”

“…Through transposon tagging using Mu transposon, Hm1 gene, a plant R-gene have been cloned for the first time in maize (Johal and Briggs 1992). Later several R-genes such as N, M, RPF, Gpa2 and others have been cloned in different crops like eggplant (Reddy et al 2015), grapevine (Donald et al 2002), citrus (Deng et al 2000) and other species (Sharma et al 2009). The comparison of the sequences of these R-genes has shown that majority possess conserved amino acid motifs consisting of a NBS domain and a hydrophobic domain (HD) with a consensus amino acid sequence, Gly-Leu-Pro-Leu (GLPL), downstream to NBS (Reddy et al 2015).…”

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

“…The first of these loci, Run1, was identified and mapped on chromosome 12 of M. rotundifolia (Pauquet et al 2001;Donald et al 2002;Merdinoglu et al 2003;Barker et al 2005). This locus was found to be closely linked to the Rpv1 locus (Merdinoglu et al 2003).…”

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

“…Erysiphe necator (isolate APC1; kindly provided by Eileen Scott, University of Adelaide) was maintained on detached leaves of Cabernet Sauvignon using an 8-to 10-d rotation as described previously (Donald et al, 2002). Leaves of potted vines of grapevine cv Cabernet Sauvignon and cv Chardonnay were inoculated with E. necator using a fine paintbrush and sampled 8 d after inoculation.…”