Identification of RFLP markers for resistance to wheat spindle streak mosaic bymovirus (WSSMV) disease

Abstract

Wheat spindle streak mosaic bymovirus (WSSMV) causes an economically important disease of winter wheat in Europe and North America. Artificial inoculation with this virus to identify resistant wheat genotypes is difficult. This study was conducted to identify restriction fragment length polymorphism (RFLP) markers associated with resistance to this disease. A population, consisting of 104 F5 recombinant inbred lines from a cross between hexaploid Triticum aestivum cultivars 'Geneva' (resistant) and 'Augusta' (susceptible), was evaluated for WSSMV symptoms under field conditions for four years. Two linked markers on the long arm of chromosome 2D, Xbcd1095 and Xcdo373, were determined to be associated with WSSMV resistance by bulked segregant analysis of the 10 most resistant and 10 most susceptible lines. Marker Xcdo373 accounted for 79% and Xbcd1095 for 73% of the phenotypic variation. Our results suggest that resistance to WSSMV in this population is qualitative in nature and is controlled by few genes. These markers should be useful in the development of wheat cultivars resistant to WSSMV and perhaps also to wheat yellow mosaic bymovirus (WYMV).

Mapping a gene conferring resistance to Wheat yellow mosaic virus in European winter wheat cultivar ‘Ibis’ (Triticum aestivum L.)

“…Genes conferring resistance to WYMV and WSSMV were previously mapped to wheat chromosome 2D in an F 2 population involving the Chinese wheat cultivar 'Yangfu 9311' (Liu et al 2005), and in F 5 recombinant inbred lines derived from the USA cultivar 'Geneva' (Khan et al 2000), respectively. Khan et al (2000) reported that the WSSMV resistance gene in 'Geneva' was flanked by restriction fragment length polymorphism markers cdo373 and bcd1095, which were 12.4 cM apart on chromosome 2D.…”

“…Khan et al (2000) reported that the WSSMV resistance gene in 'Geneva' was flanked by restriction fragment length polymorphism markers cdo373 and bcd1095, which were 12.4 cM apart on chromosome 2D. Liu et al (2005) reported that gene YmYF present in 'Yangfu 9311' was located on chromosome 2D at a distance of 8.1 cM from Xwmc41 and 11.6 cM from gwm349.…”

“…WYMV and WSSMV resistance genes were recently mapped to wheat chromosome 2D in the U.S. cultivar 'Geneva' and the Chinese cultivar 'Yangfu 9311', respectively (Khan et al 2000, Liu et al 2005. Nevertheless, the paucity of data about molecular markers linked to WYM resistance precludes their use in MAS.…”

Wheat biotechnology: A minireview

“…In wheat, RFLP's have been used to map seed storage protein loci (Dubcovsky et al 1997), loci associated with flour colour (Parker et al 1998), cultivar identification (Vaccino et al 1993), vernalization (Vrn1) and frost resistance gene on chromosome 5A (Galiba et al 1995), intrachromosomal mapping of genes for dwarfing (Rht12) and vernalization (Vrn1) (Korzun et al 1997), resistance to preharvest sprouting (Anderson et al 1993), quantitative trait loci (QTL's) controlling tissue culture response (Tcr) (Ben Amer et al 1997), nematode resistance (Eastwood et al 1994;Williams et al 1996;Lagudah et al 1997), milling yield (Parker et al 1999), resistance to chlorosis induction by Pyrenophora tritici-repentis (Faris et al 1997). RFLP markers are also useful in selection programs for resistance against pests and pathogens, which is otherwise labor and time consuming, and to detect homozygous individuals and have been used for resistance to barley yellow dwarf virus (Crasta et al 2000), resistance to wheat spindle streak mosaic virus (Khan et al 2000), resistance against powdery mildew (Hartl et al 1993;Ma et al 1994;Hartl et al 1995), resistance against leaf rust (Dedryver et al 1996;Autrique et al 1995;Feuillet et al 1995;Schachermayr et al 1995;Schachermayr et al 1994), resistance against cereal cyst nematode (Eastwood et al 1994;Lagudah et al 1997). The use of RFLP analysis in wheat has, however, been of limited use in the intervarietal analysis due to low level of polymorphism and the high cost for screening in breeding situations.…”

Development and characterization of a Triticum aestivum-Haynaldia villosa translocation line T4VS⋅4DL conferring resistance to wheat spindle streak mosaic virus

“…The only effective way to control WSSM is the development of resistant varieties and delayed planting. WSSMV resistance has been reported for several wheat varieties (Cox et al, 1994;Haufler & Fulbright, 1986;Khan et al, 2000;Cheng et al, 2000). Haynaldia villosa (syn.…”