Identification of rice hybrids and their parental lines based on seed, seedling characters, chemical tests and gel electrophoresis of total soluble seed proteins

Nethra, N.; Prasad, S. Rajendra; Vishwanath, K.; Dhanraj, K.N.; Gowda, Rame

doi:10.15258/sst.2007.35.1.16

Cited by 22 publications

(18 citation statements)

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“…Seedling storage proteins are powerful tool for the detection of the genetic diversity and also considered as reliable technology because these are highly independent on environmental fluctuations (Javid et al, 2004;Iqbal et al, 2005;Netra & Prasad, 2007;Sadia et al, 2009). Seedling storage protein profiling can be employed for various purposes, such as varietal and cultivar identification (El-Khawas & Shehata, 2005), biosystematics analysis, determination of phylogenetic relationship among different species and generation of related information to complement evaluation (Sammour, 1991;Isemura et al, 2001;Ghafoor et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Growth and Molecular Expression of Okra Seeds Interacted with Fourteen Mango Cultivars in Mixed Cropping System

Marzouk¹,

El-Darier²,

Mabrouk³

et al. 2017

JAS

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The present study was accomplished to study the effect of fourteen cultivars (Keitt, Ewais, White Succari, Tommy Atkins, Fajri Klan, Zebda, Alphonso, Sedeek, Naomi, Mesk, Baladi Dabsha, Baladi Arnaba, Cobania and Totapuri) of Mangifera indica L. leaves aqueous extracts (MILAE) on germination and some growth parameters as well as seedling protein profile of Hibiscus esculentus L. (okra seeds) in mixed cropping system. The study was extented to characterize and discriminate among the aforementioned cultivars. The allelopathic potential of fourteen cultivars of M. indica leaves aqueous extracts (MILAE) on germination efficiency and growth parameters of H. esculantus L. seeds (as a recipient bioassay material) was completely studied. Hypocotyl length (HL) was more sensitive than radicle length (RL) for all studied cultivars. At the all concentrations level, Mesk cultivar exerted the highest allelopathic effect, while Totapuri cultivar showed the lowest one on the germination percentage (GP), inhibition percentage (IP), the time to get 50% germination (T 50 ), mean germination time (MGT), germination energy (GE), seed germination index (SGI), emergence percentage (EP), mean emergence time (MET), seedling emergence index (EI), seedling vigour index (SVI), hypocotyl (HL) and radicle (RL) lengths, seedling fresh (SFW) and dry (SDW) weights.Seedling protein electrophoresis data revealed that Tommy Atkins cultivar attained the minimum values for both the number of bands and the percentage of polymorphism, 6 bands and 27%, respectively. On the other hand, the maximum values were achieved from both Naomi and Totapuri cultivars, 11 bands and 51%. Reversibly, the genomic template stability (GTS %) oscillated from 32% in Cobania cultivar to 64% in specimen Tommy Atkins cultivar. The resulted dendrograms by using allelopathic and molecular data as well as seedling protein electophoresis ascertains three aggregations. The first assembly includes Sedeek, Naomi and Mesk cultivars. The second gathers Keitt and Ewais cultivars. While, the third clusters Fajri Klan, Zebda and Alphonso cultivars.

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Section: Introductionmentioning

confidence: 99%

Growth and Molecular Expression of Okra Seeds Interacted with Fourteen Mango Cultivars in Mixed Cropping System

Marzouk¹,

El-Darier²,

Mabrouk³

et al. 2017

JAS

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“…This has promoted the search for new sources of variation that might be useful for chickpea breeding program [9]. The estimation of genetic diversity based on morphological characterization and biochemical analysis using SDS-PAGE [10] and molecular evaluation using RAPD markers [11] and SSR markers [12] have been reported and being carried out for detection of diversity in chickpea cultivars. All the techniques for estimation of genetic diversity have their own implication and validity, none is superior, but have specific implications for crop improvement or germplasm management.…”

Section: Introductionmentioning

confidence: 99%

Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding

Ahmad¹,

Mumtaz²,

Nisar³

et al. 2012

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The exploration of genetically variable accessions is the key source of germplasm conservation and potential breeding material for the future. The more diverse group of cultivars provides an ample opportunity to breeders for releasing new and superior varieties, considering their quality traits for direct commercial utilization. In this study, we assessed the genetic diversity of Cicer arietinum 70 accessions from Pakistan and USA using morphological traits, seed protein and molecular markers. Based on four morphological traits, the average coefficient of variation was calculated as 56.8% with significant correlation among yield traits. The analysis revealed that the accessions 1898, 2819, 3022, 3037, 3040, 3043, 3054, 3059 and 3063 were best in performance with a total of 12% environmental error. The cluster analysis based on protein data revealed 50% genetic diversity among accessions. The clustering pattern did not show any grouping that could be attributed to either the geographic distribution or the field performance. For molecular characterization of germplasm 5 PCR based RAPD primers, OPA4, OPA9, OPG13, UBC181 and UBC733b used were found to be polymorphic with 37% genetic diversity among local and exotic accessions. Whereas, 3 SSR primers viz., CaSTMS2, CaSTMS15 and CaSTMS21 scored the genetic variability up to 55% by cluster analysis through UPGMA percent disagreement. The primers, TA72 and TA130 were linked with yield related traits, indicated highest dissimilarity index value (0.69) and notable variation in the identified promising lines. The Morphometric, Biochemical and Molecular markers reported here, are helpfulto assess the extent of genetic diversity among Chickpea accessions and can be used to identify the unreported cultivars with desirable quantitative traits for improving Chickpea yield in Pakistan. Based on the study, the accessions 3043 and 3054 have been recommended to the breeders for their future use in multiplication and screening against various diseases.

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“…Further, phenol colour reaction of seeds seems to be due to the difference in the genetic background, presumably concerning the enzyme system (Takahashi and Hamza, 1983). The genotypes were grouped based on phenol colour reaction test in several crop palnts by Meshram and Ratiangdale (1988), Wang and Shen (1992), Vnanagmudi et al, (1988), Panwar and Ram (1988) in Wheat, Jaiswal and Agarwal (1995) and Nethra et al, (2007) in rice. In the present study, additional three more colour categories could be observed viz., light grey, grey and dark grey which was not reported by earlier studies on any crops.…”

Section: Fig1reactionofseedtostandardphenoltestmentioning

confidence: 99%

“…The difference in colour change to either NaOH/KOH was due to difference in seed constituents. One extra category dark yellow was found and was utilized for grouping of genotypes than earlier categories which werereported by many scientists (Vanangamudi et al, 1998, Nethra et al, 2007in rice Mc Donald Jr.,1985, Punia et al, 2002in wheat, Patil et al, 2006in safflower, Papp et al, 1997. However, in present study wine red category was not found in NaOH test but found in KOH test.…”

Section: Fig1reactionofseedtostandardphenoltestmentioning

confidence: 99%

“…Reaction of seeds or seedlings to different chemicals is based on seed composition; this property has been used by several researchers to characterize many crop cultivars (Papp et al, 1997, Vanangamudi et al, 1998, McDonald Jr., 1985, Punia et al, 2002, Patil et al, 2006Nethra et al, 2007). There are several tests for varietal identification and the important tests are the soybean seed coat peroxidase test, oat seed fluorescence test, KOH-bleach test for sorghum, HCl tests for oats, the phenol test for wheat, Poa pratensis and Lolium perenne, the NaOH test to distinguish red and white wheat and rice varieties, the ammonia fluorescence test to distinguish Festuca rubra and F. longifolia, CuSO 4 -ammonia test to distinguish Melilotus officinalis and M. alba, and the fluorescence test for Lolium perenne and L. multiflorum (ISTA, 1993;Mc Donald-Jr., 1985).…”

Section: Introductionmentioning

confidence: 99%

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Chemical Tests for Identification and Characterization of Tomato Cultivars

Vishwanath¹,

Pallavi²,

Nethra³

et al. 2014

Plant Breeding and Seed Science

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Various chemical tests are being used to reveal chemical differences among the seeds or seedlings of different cultivars. Study to characterize and identify 24 tomato cultivars based on chemical test chemicals viz., Standard phenol test, Modified phenol test, NaOH test, KOH test and Seedling growth response to added chemicals reveled that, most of the cultivars studied were distinct from other cultivars. No single chemical test could distinguish all the varieties. However, distinguishable chemical characteristics were used to develop the keys for identification of each and every cultivar and all the cultivars were distinguished based on these identification keys.

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Identification of rice hybrids and their parental lines based on seed, seedling characters, chemical tests and gel electrophoresis of total soluble seed proteins

Cited by 22 publications

References 1 publication

Growth and Molecular Expression of Okra Seeds Interacted with Fourteen Mango Cultivars in Mixed Cropping System

Growth and Molecular Expression of Okra Seeds Interacted with Fourteen Mango Cultivars in Mixed Cropping System

Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding

Chemical Tests for Identification and Characterization of Tomato Cultivars

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Identification of rice hybrids and their parental lines based on seed, seedling characters, chemical tests and gel electrophoresis of total soluble seed proteins

Cited by 22 publications

References 1 publication

Growth and Molecular Expression of Okra Seeds Interacted with Fourteen Mango Cultivars in Mixed Cropping System

Growth and Molecular Expression of Okra Seeds Interacted with Fourteen Mango Cultivars in Mixed Cropping System

Diversity analysis of chickpea (&lt;i&gt;Cicer arietinum&lt;/i&gt; L.) germplasm and its implications for conservation and crop breeding

Chemical Tests for Identification and Characterization of Tomato Cultivars

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Product

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Diversity analysis of chickpea (<i>Cicer arietinum</i> L.) germplasm and its implications for conservation and crop breeding