Identification of sarcosaprophagous Diptera species through DNA barcoding in wildlife forensics

Small Bait Traps as Accurate Predictors of Dipteran Early Colonizers in Forensic Studies

“…This last constraint was present in this study, as less than 50% of the specimens, previously identified morphologically, were blasted in BOLD Systems database at species level. Few molecular studies on forensically important insects using COI barcode region have been done in Portugal, except for the most abundant forensic Diptera (Cainé et al 2006(Cainé et al , 2009Ferreira et al 2011;Oliveira et al 2011;Rolo et al 2013). All sequences were added to the BOLD database and are an important contribution to future species determination through molecular methodologies.…”

“…The mitochondrial cytochrome c oxidase subunit I (COI) gene is well established for this purpose, with reference sequences available for a large number of species (Amendt et al 2011). Concerning Portugal, only a few molecular studies involving the abundant Diptera species with forensic interest have been performed (Cainé et al 2006(Cainé et al , 2009Ferreira et al 2011;Oliveira et al 2011;Rolo et al 2013). Thus, there is still a lack of COI barcode sequences available in public databases for some groups, namely several families of Diptera.…”

Molecular differentiation of Central European blowfly species (Diptera, Calliphoridae) using mitochondrial and nuclear genetic markers

“…However, morphological differences are subtle, especially in the immature stages and between closely related species [11,12]. These characteristics may be absent in damaged specimens [13,14], though DNA-based identification provides rapid and unambiguous identification in all cases.…”

Utility of GenBank and the Barcode of Life Data Systems (BOLD) for the identification of forensically important Diptera from Belgium and France

“…Moreover, the use of a reference library assembled in a different geographic area can also lead to incorrect species assignments because of geographic population structuring or eventual local hybrids (Stevens et al 2002). Therefore, surveying local entomofaunas is a prerequisite for forensic specimen identifications (Vanin et al 2008, Caine et al 2009, Rolo et al 2013). Likewise, assessing intraspecific variation and geographic substructuring is very important in forensic entomology (Wells and Williams 2007, Harvey et al 2008, Desmyter and Gosselin 2009, Sonet et al 2012).…”