Identification of small extracellular vesicle-linked miRNA specifically derived from intrafollicular cells in women with polycystic ovary syndrome

Cui, Chenchen; Wang, Jie; Han, Xiao; Wang, Qian; Zhang, Shan; Liang, Shoujing; Li, Huan; Li, Meng; Zhang, Cuilian; Chen, Huanhuan

doi:10.1016/j.rbmo.2021.02.002

Cited by 9 publications

(8 citation statements)

References 36 publications

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“…Another study found that miR-3585-5p and miR-30-5p were significantly upregulated and miR-146-5p was downregulated in the ovaries of rats with PCOS and insulin resistance (36). There are many changes in the expression of miRNA in the follicular fluid of patients with PCOS, and miR-127-3p has been found to be associated with insulin resistance (37). One study found that in PCOS ovarian granulosa cells, inhibition of long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and overexpression of miR-17-5p resulted in the downregulation of phosphatase and tensin homolog (PTEN) and promoted cell proliferation, thereby inhibiting the apoptosis of ovarian granulosa cells (38).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of microRNA in the serum of patients with polycystic ovary syndrome with insulin resistance

Huang¹,

Ji²,

Ye³

et al. 2022

Ann Transl Med

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Background: Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disease in women of childbearing age, and insulin resistance is an important etiological mechanism in PCOS. This study revealed the microRNA (miRNA) expression profile of PCOS with insulin resistance and explored the potential biological functions of differentially expressed miRNA.Methods: A total of 76 patients with PCOS and 30 normal healthy women were recruited in the gynecological clinic of the Second Hospital of Tianjin Medical University. We divided the patients with PCOS into a group with insulin resistance (n=46) and a group without insulin resistance (n=30). Peripheral venous serum samples from each group were used for deep sequencing to identify differentially expressed miRNAs. Hierarchical clustering heat maps were used to show differences in miRNA expression. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and target gene network databases were used to explore the potential target genes of differentially expressed miRNAs and to analyze their specific biological functions.Results: A case-control analysis found that the levels of body mass index (BMI), prolactin (PRL), total testosterone (T), fasting blood glucose (FBG), and fasting insulin (INS) in patients with PCOS were higher than those in healthy controls. High BMI, high blood sugar, and hyperinsulinemia were more significant in the PCOS with insulin resistance group than without insulin resistance group. Among the patients with PCOS, miR-122-5p was found to have more significant differences in the PCOS with insulin resistance group. GO and KEGG pathway analysis showed that the identified miRNAs were involved in the regulation of different biological processes, such as signal transduction, negative regulation of GTPase activity, chloride channel complex. The predicted target genes were related to the citrate cycle (TCA cycle) and the biosynthesis of mucin-type O-glycans.Conclusions: Our research demonstrated the use of miRNAs as new biomarkers for the diagnosis, treatment and presented a new strategy to lessen the symptoms of PCOS with insulin resistance.

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Section: Discussionmentioning

confidence: 99%

Differential expression of microRNA in the serum of patients with polycystic ovary syndrome with insulin resistance

Huang¹,

Ji²,

Ye³

et al. 2022

Ann Transl Med

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“…The results from the previous study in plasma were consistent with the results from this study that demonstrated that the expression of exosomal miR-106a-3p in PCOS follicular fluid was downregulated [ 16 ]. Previous research has shown that hsa-miR-34a-5p may participate in follicular development and oocyte maturation in PCOS [ 25 , 26 ]. Elevated serum-free testosterone levels in PCOS patients were significantly associated with miR-20a-5p, and circulating miRNAs may be useful as diagnostic biomarkers in PCOS women with metabolic syndrome [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Combined Transcriptomic and Metabolomic Analysis of Women with Polycystic Ovary Syndrome

Lin

Ding

et al. 2022

Disease Markers

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Background. Polycystic ovary syndrome (PCOS) is a complex class of endocrine disorders with insulin resistance, compensatory hyperinsulinemia, and obesity. However, the pathogenesis and therapies of PCOS have not been fully elucidated. Exosomal miRNAs have the potential to serve as biomarkers and therapies for a wide range of medical conditions. Method. We collected follicular fluid from 5 PCOS patients and 5 healthy people. High-throughput sequencing technology to identify differentially expressed miRNAs and untargeted metabolome identify differential metabolites in follicular fluid exosomal. RT-qPCR and AUC analysis were performed. Result. miRNA high-throughput sequencing identified 124 differential miRNAs. RT-qPCR analysis confirmed the sequencing results. These differential miRNA target genes are mainly involved in metabolic pathways. Metabolomics studies identified 31 differential metabolites. miRNA and lncRNA coexpression networks in metabolic pathways rigorously screened 28 differentially expressed miRNAs. This network would identify miRNA signatures associated with metabolic processes in PCOS. Meanwhile, the area under curve of receiver operating characteristic revealed that hsa-miR-196a-3p, hsa-miR-143-5p, hsa-miR-106a-3p, hsa-miR-34a-5p, and hsa-miR-20a-5p were potential biomarkers for the diagnosis of PCOS. Conclusion. Collectively, these results demonstrate the potential pathogenesis of PCOS, and follicular fluid exosomal miRNAs may be efficient targets for the diagnosis and treatment of PCOS in long-term clinical studies.

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“…The smallest subpopulation of which are called exosomes, and they are known to act as a mediator to transport and release miRNAs between target cells [12]. It has been reported that miRNAs are more stable in exosomes with greater gene regulatory activity [13]. miRNA's functions become feasible through participation in processes involved in biological growth, development and disease state.…”

Section: Mirnas As a Potential Novel Clinical Biomarker For Pcosmentioning

confidence: 99%

“…It allows for an efficient cross talk between the blood, granulosa and TCs [9]. FF comprises various hormones, multiple proteins, metabolites, antiapoptotic factors and regulatory nucleotides including miRNAs [13]. Therefore, FF composition reflects the secretory activity of oocyte, GC and TCs.…”

Section: Mirna In Follicular Fluid and Pcosmentioning

confidence: 99%

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The Novelty of miRNAs as a Clinical Biomarker for the Management of PCOS

Alhamdan¹,

Hernandez-Medrano²

2022

Polycystic Ovary Syndrome - Functional Investigation and Clinical Application

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Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects around 5–10% of women of reproductive age. The aetiology of PCOS is not fully understood with various genetics, iatrogenic (e.g. chemotherapy) and environmental factors have been proposed. microRNAs (miRNAs) are small non-coding single-stranded RNAs which are known to act as a regulator to gene expression at the post-transcriptional levels. Altered expression of miRNAs has been linked to several disorders including infertility. Recent reports demonstrated the expression of differential levels of miRNAs in the serum, ovarian follicular cells and follicular fluid of PCOS patients when compared with healthy women. Therefore, miRNAs may play important role in the pathogenesis of PCOS. The aim of this chapter is to summarise the current understanding pertaining to miRNAs and PCOS and to expedite its possible role in the diagnosis and management of this disorder.

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Identification of small extracellular vesicle-linked miRNA specifically derived from intrafollicular cells in women with polycystic ovary syndrome

Cited by 9 publications

References 36 publications

Differential expression of microRNA in the serum of patients with polycystic ovary syndrome with insulin resistance

Differential expression of microRNA in the serum of patients with polycystic ovary syndrome with insulin resistance

Combined Transcriptomic and Metabolomic Analysis of Women with Polycystic Ovary Syndrome

The Novelty of miRNAs as a Clinical Biomarker for the Management of PCOS

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