Identification of SNAT Family Genes Suggests GhSNAT3D Functional Reponse to Melatonin Synthesis Under Salinity Stress in Cotton

Zhang, Yuexin; Rui, Cun; Fan, Yapeng; Xu, Nan; Hong, Zhang; Wang, Jing; Sun, Liangqing; Mei, Dai; Ni, Kesong; Chen, Xiugui; Lu, Xuke; Wang, Delong; Wang, Shuai; Guo, Lixue; Zhao, Lingxia; Feng, Xixian; Chen, Chao; Ye, Wuwei

doi:10.3389/fmolb.2022.843814

Cited by 16 publications

(14 citation statements)

References 63 publications

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“…The determination of Ca 2+ content was performed according to the method we reported previously [ 38 ]. The detection of NO and proline contents and SOD activity were performed by using the nitric oxide (NO) assay kit, proline assay kit and superoxide dismutase (SOD) activity assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive genomic characterization of cotton cationic amino acid transporter genes reveals that GhCAT10D regulates salt tolerance

Chen

Yin

et al. 2022

BMC Plant Biol

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Background The cationic amino acid transporters (CAT) play indispensable roles in maintaining metabolic functions, such as synthesis of proteins and nitric oxide (NO), biosynthesis of polyamine, and flow of amino acids, by mediating the bidirectional transport of cationic amino acids in plant cells. Results In this study, we performed a genome-wide and comprehensive study of 79 CAT genes in four species of cotton. Localization of genes revealed that CAT genes reside on the plasma membrane. Seventy-nine CAT genes were grouped into 7 subfamilies by phylogenetic analysis. Structure analysis of genes showed that CAT genes from the same subgroup have similar genetic structure and exon number. RNA-seq and real-time PCR indicated that the expression of most GhCAT genes were induced by salt, drought, cold and heat stresses. Cis -elements analysis of GhCAT promoters showed that the GhCAT genes promoters mainly contained plant hormones responsive elements and abiotic stress elements, which indicated that GhCAT genes may play key roles in response to abiotic stress. Moreover, we also conducted gene interaction network of the GhCAT proteins. Silencing GhCAT10D expression decreased the resistance of cotton to salt stress because of a decrease in the accumulation of NO and proline. Conclusion Our results indicated that CAT genes might be related with salt tolerance in cotton and lay a foundation for further study on the regulation mechanism of CAT genes in cationic amino acids transporting and distribution responsing to abiotic stress. Supplementary Information The online version contains supplementary material available at 10.1186/s12870-022-03829-w.

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Section: Methodsmentioning

confidence: 99%

Comprehensive genomic characterization of cotton cationic amino acid transporter genes reveals that GhCAT10D regulates salt tolerance

Chen

Yin

et al. 2022

BMC Plant Biol

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“…In addition, collinearity analysis showed that a large number of tandem repeats occurred between homologous chromosomes. These results suggest that modes of gene family expansion, including duplication, and multiple gene copies prevent gene mutation-induced gene function loss, which indicates the importance of their function ( Xu et al., 2012 ; Zhang et al., 2022 ). Among these genes, GhSBT26 and GhSBT27A are tandemly repeated genes.…”

Section: Discussionmentioning

confidence: 84%

Genome-wide analysis of the SBT gene family involved in drought tolerance in cotton

Mei

Zhou²,

Zhang³

et al. 2023

Front. Plant Sci.

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The subtilisin-like proteases (SBTs) are a large family of serine peptidases that are unique to plants. Previous studies have shown that SBTs are associated with developmental processes and environmental responses. However, comprehensive identification and systematic analysis of the SBT family have not been conducted in cotton. We used bioinformatics methods to analyze the structural characteristics, phylogenetic relationships, gene structures, expression modes, evolutionary relationships, selection pressures and stress responses of SBT gene family members in upland cotton. In this study, we identified 120 and 112 SBTs in the tetraploid cotton species G. hirsutum and G. barbadense, while 67 and 69 SBTs were identified in the diploid species G. arboreum and G. raimondii, respectively; these SBTs were divided into five distinct subfamilies. We identified the SBT gene GhSBT27A, and explore its function through virus-induced gene silencing and transmission electron microscopy. These results suggested that the GhSBT27A gene was involved in the response to drought stress. These results lay a foundation for further study on the drought stress mechanism of cotton.

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“…The GhGAD protein interaction network was analyzed through the STRING database ( https://string-db.org/ ) ( Zhang et al, 2022 ). On the basis of A. thaliana orthologs to predict the interaction of GhGAD family genes with other genes in cotton.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton

Huang

Cui

et al. 2022

Front. Genet.

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Glutamate decarboxylase (GAD) mainly regulated the biosynthesis of γ-aminobutyric acid (GABA) and played an important role in plant growth and stress resistance. To explore the potential function of GAD in cotton growth, the genome-wide identification, structure, and expression analysis of GAD genes were performed in this study. There were 10, 9, 5, and 5 GAD genes identified in G. hirsutum, G. barbadense, G. arboreum, and G. raimondii, respectively. GAD was divided into four clades according to the protein motif composition, gene structure, and phylogenetic relationship. The segmental duplication was the main way of the GAD gene family evolution. Most GhGADs respond to abiotic stress. Clade Ⅲ GAD was induced by Cd2+ stress, especially GhGAD6, and silencing GhGAD6 would lead to more serious Cd2+ poisoning in cotton. The oxidative damage caused by Cd2+ stress was relieved by increasing the GABA content. It was speculated that the decreased expression of GhGAD6 reduced the content of GABA in vivo and caused the accumulation of ROS. This study will further expand our understanding of the relationship between the evolution and function of the GhGAD gene family and provide new genetic resources for cotton breeding under environmental stress and phytoremediation.

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Identification of SNAT Family Genes Suggests GhSNAT3D Functional Reponse to Melatonin Synthesis Under Salinity Stress in Cotton

Cited by 16 publications

References 63 publications

Comprehensive genomic characterization of cotton cationic amino acid transporter genes reveals that GhCAT10D regulates salt tolerance

Comprehensive genomic characterization of cotton cationic amino acid transporter genes reveals that GhCAT10D regulates salt tolerance

Genome-wide analysis of the SBT gene family involved in drought tolerance in cotton

Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton

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